Nutrient-dependent regulation of neural stem cell proliferation and neural circuit formation

神经干细胞增殖和神经回路形成的营养依赖性调节

• 批准号: 10810111
• 负责人: Sarah Elizabeth Siegrist
• 金额: $ 1.18万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10810111
• 关键词: Alzheimer' s Disease; Animal Feed; Biological Assay; Candidate Disease Gene; Collection; Colony-Forming Units Assay; Data; Development; Disease; Drosophila melanogaster; Genes; Genome; Goals; Healthcare Systems; Human; Inbreeding; Malignant Neoplasms; Microcephaly; Microscopy; Mitotic; Neurodegenerative Disorders; Neurodevelopmental Disorder; Nutrient; Proliferating; Research; Research Project Grants; Societies; Stem Cell Research; Students; Training; Writing; autism spectrum disorder; career development; cost; fly; genome analysis; genome wide association study; model organism; nerve stem cell; neural circuit; neuroregulation; premature; screening; skills; stem cell proliferation; stem cells; summer research; tumorigenesis; undergraduate student

Project Summary The goal of this summer research project is to identify genes that regulate the reactivation of neural stem cells from developmental quiescence using the model organism Drosophila melanogaster. Quiescence is a fundamentally important stem cell state in which stem cells are mitotically dormant. Mitotic dormancy protects stem cells from uncontrolled proliferation which could lead to premature depletion of the stem cell pool or tumorigenesis. To identify genes that regulate neural stem cell reactivation from quiescence, a collection of inbred wildtype flies will be screened whose genomes have been sequenced. Reactivation from quiescence will then be assayed in each line by feeding animals EdU and numbers of proliferating neural stem cells assayed. A genome wide association study will then be carried out and a list of candidate genes will be generated. Next steps will include screening the candidate gene list. This project is aimed at providing training for one undergraduate student during the summer months. This project will provide the student both technical training (working with model organisms, microscopy, and analysis of genome sequences) and non-technical, career development activities, including data presentation and development of writing skills.

项目概要 今年夏天的研究项目的目标是识别调节神经元重新激活的基因。 使用模式生物果蝇从发育静止期获得干细胞。 静止是一种极其重要的干细胞状态，其中干细胞处于有丝分裂休眠状态。 有丝分裂休眠可保护干细胞免于不受控制的增殖，从而导致早熟 干细胞库的耗尽或肿瘤发生。鉴定调节神经干细胞的基因 从静止状态重新激活，将筛选一组近交野生型果蝇，其基因组 已被测序。然后将通过喂食来测定每条线从静止状态的重新激活 分析动物的 EdU 和增殖神经干细胞的数量。全基因组关联 然后将进行研究并生成候选基因列表。下一步将包括 筛选候选基因列表。该项目旨在为一名本科生提供培训 暑假期间的学生。该项目将为学生提供技术培训 （使用模型生物、显微镜和基因组序列分析）和非技术， 职业发展活动，包括数据呈现和写作技能的发展。

项目成果

Delta-dependent Notch activation closes the early neuroblast temporal program to promote lineage progression and neurogenesis termination in Drosophila.

Delta依赖性Notch激活关闭早期神经母细胞时间程序，促进果​​蝇谱系进展和神经发生终止。

• DOI: 10.1101/2023.03.28.534626
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Sood,Chhavi;Nahid,MdAusrafuggaman;Branham,KendallR;Pahl,MatthewC;Doyle,SusanE;Siegrist,SarahE
• 通讯作者: Siegrist,SarahE

Notch signaling regulates neural stem cell quiescence entry and exit in Drosophila.

Notch 信号传导调节果蝇神经干细胞静止的进入和退出。

• DOI: 10.1242/dev.200275
• 发表时间: 2022
• 期刊: Development (Cambridge, England)
• 作者: Sood,Chhavi;Justis,VirginiaT;Doyle,SusanE;Siegrist,SarahE
• 通讯作者: Siegrist,SarahE

Steroid hormones, dietary nutrients, and temporal progression of neurogenesis.

• DOI: 10.1016/j.cois.2020.10.008
• 发表时间: 2021-03
• 期刊: Current opinion in insect science
• 影响因子: 5.3
• 作者: Sood C;Doyle SE;Siegrist SE
• 通讯作者: Siegrist SE

Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants.

• DOI: 10.3791/63189
• 发表时间: 2022-05-18
• 期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
• 影响因子: 1.2
• 作者: Keliinui, Cami Naomi;Doyle, Susan E.;Siegrist, Sarah E.
• 通讯作者: Siegrist, Sarah E.