Cell growth and proliferation control in neural stem cells

项目摘要： 这个研究项目的长期目标是了解营养来源的线索如何整合 用细胞内在的程序来控制神经发生的时间和速度。我们大脑中的所有神经元 大脑是由神经干细胞的不对称细胞分裂产生的， 更新多能祖细胞。神经干细胞在调节大脑大小方面起着关键作用， 神经元的多样性，因为这个群体始终积极参与细胞周期 发展虽然许多努力都是为了识别基因和细胞， 信号通路调节神经干细胞的自我更新，很少有人知道如何 外源性因素，局部和全身，与内在的神经干细胞因子整合，以控制 神经发生的时间和速率。在这里，使用果蝇的大脑作为一个遗传上易于处理的 模型系统中，我们研究了营养来源的外源信号如何与细胞内源信号整合， 程序来控制神经发生的时间和速度。 在这个建议中，我们将研究（1）驱动不对称的机制是否 细胞分裂本身也在调节神经干细胞的生长和增殖中发挥作用，（2）如何 营养调节神经干细胞增殖，以及（3）特定的转录因子， 它是高度保守的，可以超越神经干细胞对营养的依赖性需求， 增殖

Project Summary: The long term goal of this research project is to understand how nutrient derived cues integrate with cell intrinsic programs to control timing and rate of neurogenesis. All neurons within our brains are generated from the asymmetric cell divisions of neural stem cells, which are self- renewing multi-potent progenitors. Neural stem cells play a key role in regulating brain size and neuronal diversity, as this population remains actively engaged in the cell cycle throughout development. While much effort has been geared towards identifying genes and the cell signaling pathways that regulate neural stem cell self-renewal, less is known about how extrinsic factors, local and systemic, integrate with intrinsic neural stem cell factors to control timing and rate of neurogenesis. Here, using the Drosophila brain as a genetically tractable model system, we investigate how nutrient derived extrinsic signals integrate with cell intrinsic programs to control timing and rate of neurogenesis. In this proposal, we will investigate (1) whether the machinery that drives asymmetric cell division itself also play a role in regulating neural stem cell growth and proliferation, (2) how nutrition regulates neural stem cell proliferation, and (3) how a particular transcription factor, which is highly conserved, can override nutrient-dependent requirements for neural stem cell proliferation.