In Situ Hybridization and Immunohistochemistry Core

原位杂交和免疫组织化学核心

• 批准号: 8377527
• 负责人: ASHLEY T. HAASE
• 金额: $ 25.51万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8377527
• 关键词: Anatomic Sites; Animal Model; Biological Assay; Cell Culture Techniques; Cell model; Cells; Gene Expression; Goals; HIV; HIV Infections; HIV-1; Highly Active Antiretroviral Therapy; Human; Human Cell Line; Immunohistochemistry; In Situ; In Situ Hybridization; Infection; Instruction; Modeling; Mus; Research Personnel; Research Project Grants; Response Latencies; SIV; Technology; Testing; Therapeutic; Therapeutic Agents; Tissues; Viral; Viral Genes; antiretroviral therapy; collaboratory; design; drug candidate; latent infection; mouse model; nonhuman primate; response; simian human immunodeficiency virus; therapy design; tool

Understanding the cellular and tissue reservoirs of HIV-1 infection before and during H/\ART, and the responses in cell culture and animal models to therapies that reactivate viral gene expression in latently infected cells should enable design of therapies that ultimately aim to eradicate HIV-1 infection, In situ hybridization, in situ PCR, immunohistochemistry and combinations thereof will provide investigaors in the collaboratory with the tools to characterize cell and tissue reseroirs in the non-human primate model, and the response to therapies that induce reactivation in the animal model and cell culture model.

在H/ART之前和期间了解HIV-1感染的细胞和组织储存库， 在细胞培养和动物模型中对治疗的反应， 受感染的细胞应该能够设计出最终旨在根除HIV-1感染的治疗方法， 杂交、原位PCR、免疫组织化学及其组合将为研究者提供在本领域中的应用。 合作实验室的工具，以表征细胞和组织库在非人类灵长类动物模型， 在动物模型和细胞培养模型中，细胞对诱导再活化的疗法的应答。