Prokineticin 2 and Neuroinflammatory Mechanisms

Prokineticin 2 和神经炎症机制

• 批准号: 8273762
• 负责人: ARTHI KANTHASAMY
• 金额: $ 31.88万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8273762
• 关键词: 1-Methyl-4-phenylpyridinium; Address; Animal Model; Anti-Inflammatory Agents; Anti-inflammatory; Apoptotic; Astrocytes; Autopsy; Biological Assay; Brain; Brain region; Cell Culture Techniques; Cells; Coculture Techniques; DNA Fragmentation; Data; Dendroaspis; Disease; Encephalitis; Gene Expression; Goals; Homologous Protein; Inflammatory; Measures; Mediating; Microglia; Midbrain structure; Migration Assay; Modeling; Molecular; Molecular Profiling; Mus; Nerve Degeneration; Neurodegenerative Disorders; Neuroglia; Neurons; Parkinson Disease; Pathway interactions; Patients; Pattern; Physiological; Primary Cell Cultures; Process; Proteins; Recombinants; Reporter; Research; Role; Sampling; Severities; Signal Transduction; Signaling Molecule; Small Interfering RNA; Snake Venoms; Staging; Stimulus; Stress; System; Tissues; Transduction Gene; Transgenic Mice; Up-Regulation; Viral; Wound Healing; caspase-3; chemokine; clinically relevant; clinically significant; cytokine; dopaminergic neuron; in vivo; insight; knockout animal; migration; neurochemistry; neuroinflammation; neuron loss; neurotoxic; novel; novel therapeutic intervention; novel therapeutics; overexpression; promoter; receptor; release factor; response; treatment strategy

DESCRIPTION (provided by applicant): Neuroinflammation is now recognized as a key degenerative mechanism in several neurodegenerative diseases including Parkinson's disease (PD). The severity of neuronal damage caused by neuroinflammatory stress is dependent on the degree of dysregulation of inflammatory and anti-inflammatory pathways in brain. Most studies to date are focused on identifying major pro-inflammatory pathways that are activated during neuroinflammatory insult. Understanding the anti-inflammatory mechanisms associated with various stages of brain inflammation will provide new insights into disease processes associated with neurodegenerative diseases. In this proposal, we aim to delineate a novel anti-inflammatory protective response in the nigrostriatal dopaminergic neurons mediated by the recently discovered mammalian protein homolog of mamba snake venom: Prokineticin-2 (PK2). Unexpectedly, we observed a dramatic up-regulation of PK2 protein and its release from dopaminergic neuronal cells during inflammatory TNF¿ insult. Further observation of increased PK2 expression in nigral dopaminergic neurons in an animal model of PD as well as in the nigral samples from postmortem PD patients provides credence for the clinical significance of our findings. Interestingly, recombinant PK2 significantly protected against apoptotic neuronal cell death and TH positive dopaminergic neuronal loss induced by neuroinflammatory insults. Surprisingly, PK2 treatment also promoted migration of both astrocytes and microglial cells. Therefore, in this proposal, we intend to expand our preliminary observations by pursuing the following specific aims: (i) To characterize the PK2 induction, release and function in dopaminergic neurons following inflammatory insults in primary cell culture and animal models, (ii) To determine the effect of PK2 induction on astroglial and microglial migration and function following inflammatory stimuli, (iii) To investigate the molecular mechanisms of PK2 up-regulation in neuronal cells, and (iv) To demonstrate the neuroprotective effect of PK2 in primary cell culture and animal models of PD. Cellular, molecular and neurochemical approaches will be used to delineate these specific aims. Together, understanding the role of PK2 up-regulation and release during inflammatory stress in dopaminergic neurons will not only provide new insights about neurodegenerative mechanisms underlying nigral dopaminergic degeneration but may also yield novel therapeutic strategies for treatment of Parkinson's disease. PUBLIC HEALTH RELEVANCE: Brain inflammation is emerging as a central mechanism underlying the progression of many neurodegenerative diseases including Parkinson's disease. However, the cellular factors released from dopaminergic neurons during inflammatory stress that initiate the glial cell-mediated early compensatory protective response are yet to be identified. In this proposal, we characterize the protective function of a novel signaling molecule prokineticin-2 in cell culture and animal models. Thus, mechanistic understanding of protkineticin-2 in dopaminergic neurons and glial cells following inflammatory and neurotoxic insults could provide new therapeutic approaches for impeding the progression of PD.

描述（由申请人提供）：神经炎症现在被认为是包括帕金森病（PD）在内的几种神经退行性疾病的关键退行性机制。神经炎症应激引起的神经元损伤的严重程度取决于脑内炎症和抗炎通路的失调程度。迄今为止，大多数研究都集中在确定在神经炎症损伤期间激活的主要促炎途径。了解与脑炎症各阶段相关的抗炎机制将为与神经退行性疾病相关的疾病过程提供新的见解。在这项提议中，我们的目标是描述黑质纹状体多巴胺能神经元中一种新的抗炎保护反应，这种反应是由最近发现的哺乳动物曼巴蛇毒同源蛋白：Prokineticin-2 （PK2）介导的。出乎意料的是，我们观察到在炎症性TNF损伤期间，多巴胺能神经元细胞中PK2蛋白及其释放的显著上调。进一步观察PD动物模型和PD患者死后神经样本中黑质多巴胺能神经元中PK2表达的增加，为我们的研究结果提供了临床意义。有趣的是，重组PK2显著保护神经炎症损伤诱导的凋亡神经元细胞死亡和TH阳性多巴胺能神经元损失。令人惊讶的是，PK2处理也促进了星形胶质细胞和小胶质细胞的迁移。因此，在这项建议中，我们打算扩大我们的初步意见，力求达到下列具体目标：(1)在原代细胞培养和动物模型中表征炎症损伤后多巴胺能神经元中PK2的诱导、释放和功能；(2)确定炎症刺激后PK2诱导对星形胶质细胞和小胶质细胞迁移和功能的影响；(3)研究神经元细胞中PK2上调的分子机制；(4)在PD的原代细胞培养和动物模型中证明PK2的神经保护作用。细胞、分子和神经化学的方法将被用来描述这些特定的目标。总之，了解多巴胺能神经元炎症应激过程中PK2上调和释放的作用，不仅将为神经性多巴胺能变性的神经退行性机制提供新的见解，而且可能为治疗帕金森病提供新的治疗策略。