OPTICAL DIFFRACTION TOMOGRAPHY FOR HIGH RESOLUTION 3D LIVE CELL IMAGING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 层析相位显微术（TPM）是一种将定量相位成像与层析成像方法相结合的独特装置，它在入射光束的多个照射角度下获取定量相位图像，并通过对二维相位图像进行数值处理来重建三维折射率图。活细胞的三维成像提供了一种研究单个细胞器的光学特性的方法。该项目的目标是以高空间分辨率和准确度测量活细胞的折射率图。所涉及的挑战是来自内部细胞器的衍射和入射光束的有限角度覆盖。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Tomographic phase microscopy (TPM) is a unique device that combines the quantitative phase imaging with tomographic method for 3-D reconstruction: quantitative phase images are taken at multiple illumination angles of an incident beam and the 3-D refractive index map is reconstructed by numerically processing the 2-D phase images. Three dimensional imaging of a live cell provides a way to investigate optical properties of individual organelles. The aim of the project is to measure refractive index map in live cells with high spatial resolution and accuracy. The challenges involved are diffraction from internal organelles and limited angular coverage of the incident beam.