DISPERSION TOMOGRAPHY FOR DNA QUANTIFICATION OF A LIVE CELL

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的主要动机是在不添加任何额外试剂的情况下定量活细胞内的DNA量。DNA的折射率在紫外线附近还没有被精确测量，但报道的吸收光谱表明它具有与蛋白质不同的色散特性。因此，通过在两个或更多个不同波长下测量活细胞的折射率图，我们期望量化活细胞内DNA的三维分布。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary motivation of this project is to quantify the DNA quantity within a live cell without adding any extra agent. The refractive index of DNA has not been accurately measured near the ultraviolet range, but the reported absorption spectra suggest it has different dispersion characteristics from proteins. Hence, by measuring the refractive index map of a live cell at two or more different wavelengths, we expect to quantify three dimensional distribution of DNA within a live cell.