DISPERSION TOMOGRAPHY FOR DNA QUANTIFICATION OF A LIVE CELL

用于活细胞 DNA 定量的色散断层扫描

• 批准号: 8364152
• 负责人: Sung Mi Yom
• 金额: $ 2.97万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364152
• 关键词: Biomedical Research; Cells; Characteristics; DNA; Funding; Grant; Lasers; Life; Maps; Measures; Motivation; National Center for Research Resources; Principal Investigator; Proteins; Refractive Indices; Reporting; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; absorption; cost; tomography; ultraviolet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary motivation of this project is to quantify the DNA quantity within a live cell without adding any extra agent. The refractive index of DNA has not been accurately measured near the ultraviolet range, but the reported absorption spectra suggest it has different dispersion characteristics from proteins. Hence, by measuring the refractive index map of a live cell at two or more different wavelengths, we expect to quantify three dimensional distribution of DNA within a live cell.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目的主要动机是在不添加任何额外试剂的情况下定量活细胞内的DNA量。DNA的折射率在紫外线附近还没有被精确测量，但报道的吸收光谱表明它具有与蛋白质不同的色散特性。因此，通过在两个或更多不同波长下测量活细胞的折射率图，我们希望量化活细胞内DNA的三维分布。