SIDE-VIEWING RAMAN OPTICAL FIBER PROBE FOR BIOMEDICAL SPECTROSCOPY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们正在研究使用拉曼光谱来指导乳腺立体定向核心针活检筛查乳房X光检查中发现的可疑微钙化。微钙化是乳腺组织中钙矿物盐的沉积，通过乳房X光检查可以定位最显著的临床异常，因此是后续针吸活检的目标。 拉曼光谱对含钙矿物的存在非常敏感，因此可以用来检测和定位乳房中的微钙化。这可以通过与现有针吸活组织检查设备兼容的光纤探头，以非破坏性的方式在体内实时完成。侧视拉曼光纤探头提供了利用来自拉曼源的激发光，并在探头插入针头装置时将光传输到乳腺组织并从乳腺组织收集光的能力。该探头被设计成适合于在立体定向核心针活检中最常用的9号针装置。与已成功用于体内和体外临床研究的前视拉曼探头类似，侧视探头将激发和发射滤光片与涂层结合在一起，以抑制光纤背景并实现有效的拉曼信号提取。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are investigating the usage of Raman spectroscopy to guide stereotactic core needle biopsies of the breast for suspicious microcalcifications discovered at screening mammography. Microcalcifications are deposits of calcium mineral salts within breast tissue that mammographically localize the most clinically significant abnormality and are, therefore, a target for subsequent needle biopsy. Raman spectroscopy is exquisitely sensitive to the presence of calcium-containing minerals, and thus can be used to detect and localize microcalcifications in the breast. This can be done in vivo, in real-time, in a non-destructive manner, via optical fiber probes that are compatible with existing needle biopsy devices. The side-viewing Raman fiber optic probe provides capability of utilizing the excitation light from Raman source and delivering to and collecting the light from the breast tissue while the probe inserted into needle device. The probe is designed to fit into 9 gauge needle device most commonly used during stereotactic core needle biopsies. Similar to the front-viewing Raman probe that has been successfully used in the in vivo and ex vivo clinical studies, the side-viewing probe incorporate excitation and emission filters with coatings to suppress fiber background and enable efficient extraction of the Raman signal.