EX VIVO CLINICAL RAMAN SPECTROSCOPY OF BREAST CANCER

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心赠款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 我们已经证明了使用拉曼光谱法在诊断新鲜切除组织上的正常、两种类型的良性病变和恶性病变方面的优异结果。因此，该研究支持将该技术转移到临床环境中，以进一步测试其在乳腺癌诊断中的有效性。因此，我们进行了临床研究。我们在克利夫兰大学医院病理室收集数据，通常在部分和整个乳房切除术中切除半小时内。本研究的主要目标是验证我们的体外诊断算法在一个大的数据集，密切模仿体内环境，并阐明新鲜冷冻和新鲜乳腺组织的拉曼光谱之间的差异。计划进行另一项临床研究。本研究的主要目标是扩展我们的诊断算法，以包括以前在我们的研究中没有看到的病理，如导管原位癌和微钙化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have demonstrated excellent results in diagnosing normal, two types of benign lesions and malignant lesions on freshly excised tissue using Raman spectroscopy. The study thus supported moving the technique to a clinical setting for further testing of its efficacy in breast cancer diagnosis. As a result, we pursued clinical study. We collected data in the University Hospitals of Cleveland pathology suite, typically within half an hour of excision during partial and whole mastectomy. The main goals of this study was to validate our in vitro diagnostic algorithm on a large data set which closely mimics an in vivo environment and to elucidate differences between the Raman spectra of fresh-frozen and fresh breast tissues. Another clinical study is scheduled. The main goal of this study is expanding our diagnostic algorithm to include pathologies not seen previously in our studies, such as ductal carcinoma in situ and microcalcifications.