Scientific Core: Bacillus anthracis Anthrax Toxin Core

炭疽毒素的作用和使用毒素成分作为炭疽疫苗的可能性是 这是本提案中许多拟议项目的核心。这一核心将为调查人员提供高度 纯化的炭疽毒素蛋白，可在大小规模的研究中持续使用。毒素核心 会提纯大量的PA、LF和EF，这是无内毒素的。一批批毒素将由核进行测试，以确定 将提供相对活性和标准化的毒素批次，以便调查人员可以进行多项研究 在这个项目的五年中，对这种试剂的一致性充满信心。此核心还将存储和 为PA、LF和EF编目，并雇用一名技术人员来监督净化、表征和 毒素的分布。

The role of anthrax toxin and the potential for using components of the toxin as an anthrax vaccine, are central to many of the proposed projects in this proposal. This core will provide investigators with highly purified anthrax toxin proteins that can be used consistently in large and small-scale studies. The toxin core will purify large amounts of PA, LF, and EF, that is LPS-free. Batches of toxin will be tested by the core for relative activity and normalized batches of toxin will be provided, so investigators can pursue multiple studies over the five years of this project confident in the consistency of this reagent. This core will also store and catalog PA, LF, and EF and employee a technician that will oversee the purification, characterization, and distribution of the toxin.