Oklahoma C. difficile U19 Challenge Core

俄克拉荷马州艰难梭菌 U19 挑战核心

• 批准号: 10625174
• 负责人: Jimmy D. Ballard
• 金额: $ 7.32万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-25 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10625174
• 关键词: Animal Model; Antibiotic Therapy; Antibiotics; Clostridium difficile; Data; Dedications; Disease; Dose; Ensure; Environment; Generations; Goals; Hamsters; Health; Individual; Infection; Joints; Laboratories; Measures; Methods; Modality; Modeling; Monitor; Mus; Oklahoma; Oral; Outcome; Predisposition; Procedures; Public Health; Publications; Reagent; Recurrence; Recurrent disease; Reproducibility; Reproduction spores; Research; Research Personnel; Research Project Grants; Services; Standardization; Vaccinated; Vaccination; Vaccines; Variant; Withdrawal; efficacy testing; experience; experimental study; gut microbiome; interest; microbiome; mouse model; pathogen; programs; recurrent infection; vaccine candidate; vaccine efficacy; vaccine evaluation; vaccine response

Project Summary (Challenge Core) The central goal of the Oklahoma C. difficile U19 program is to advance a second-generation vaccine for C. difficile by revealing mechanisms that limit protective vaccine-induced responses. In order to achieve these goals, challenge experiments are required whereby relevant animal models are used to test vaccine efficacy. Given that the U19 program has 3 distinct but complementary Research Projects and that synergistic interaction between those projects is desirable, standardization of the challenge experiments is a prudent and essential measure. Towards, this goal, we will build upon our experience with murine and hamster challenge models to develop a coordinated ‘Challenge Core’ which will provide a standardized service to each of the Research Projects. In Specific Aim 1, a single infection murine challenge model will be further developed as a core service. In Specific Aim 2, a repeat infection model will be further optimized and provided as a core service. In Specific Aim 3, a hamster challenge model will be established and provided as a core service for promising vaccination modalities. Importantly, all approaches are utilized currently by the Ballard and/or Lang laboratories, but will be further developed to ensure standardization and experimental rigor with regard to environment, microbiome, reagents, methods, and readouts. A dedicated core will also relieve the burden on individual projects and facilitate rigorous experimentation. The core service will be made available to other investigators interested in C. difficile research and will lower the barrier to setting up challenge experiments for specific projects.

挑战核心（Challenge Core） 俄克拉荷马州C的中心目标。艰难梭菌U19项目是为了开发第二代艰难梭菌疫苗。 通过揭示限制疫苗诱导的保护性反应的机制来解决这一难题。为了实现这些 为了达到这一目标，需要进行攻击实验，利用相关的动物模型来测试疫苗的效力。 鉴于U19计划有3个不同但互补的研究项目， 在这些项目之间是可取的，挑战实验的标准化是谨慎和必要的， measure.为了实现这一目标，我们将利用小鼠和仓鼠激发模型的经验， 开发一个协调的“挑战核心”，为每个研究项目提供标准化服务。 项目在具体目标1中，将进一步开发单一感染鼠攻毒模型作为核心服务。 在具体目标2中，重复感染模型将进一步优化并作为核心服务提供。在特定 目标3，建立仓鼠攻击模型，并作为有前景的疫苗接种的核心服务提供 方式。重要的是，所有方法目前都被Ballard和/或Lang实验室利用，但将在2015年10月15日之前被重新使用。 进一步发展，以确保环境，微生物组， 试剂、方法和读数。一个专用的核心还将减轻个别项目的负担， 促进严格的实验。核心服务将提供给其他对C. 这将减少艰难的研究，并将降低为特定项目建立挑战性实验的障碍。