CORE B - ANIMAL CORE

核心 B - 动物核心

• 批准号: 7683522
• 负责人: Francesco John DeMayo
• 金额: $ 11.52万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7683522
• 关键词: Animal Experimentation; Animal Model; Animals; Biology; Biomedical Research; Cells; DNA; Embryo; Endometrial; Engineering; Ensure; Equipment; Equipment and supply inventories; Generations; Genetically Engineered Mouse; Human; Investigation; Microinjections; Microscopy; Modeling; Mus; Mutant Strains Mice; Mutation; Reagent; Regulation; Reproduction; Reproductive Biology; Research; Research Personnel; Research Project Grants; Research Technics; Resources; Source; Structure of thyroid parafollicular cell; System; Technical Expertise; Techniques; Tissues; Training; Transgenic Mice; Xenograft procedure; animal resource; biological research; cost; embryonic stem cell; endometriosis; in vivo; male; mouse model; programs; reconstitution; repository; reproductive; tissue culture

The Animal Core will serve as a resource to supply Center investigators with the necessary animals and animal research techniques to effectively execute their research. The Animal Core will be responsible for the purchasing and inventory of the animals used by Center investigators, as well as, providing investigators with the technical ability required in the manipulation of the various animal models. In the past the Animal Core was responsible for the generation of genetically engineered mice. However, the focus of the research in this center application will be on investigation of endometrial biology in mouse models already generated. Therefore, the emphasis of this Core in the renewal will be to serve as a repository for the animals used in the core and for the phenotypic analysis of impact of the genetic alterations on mouse reproductive biology. This Core will incorporate the use of xenotransplants of human endometrial tissue into NOD/SCID/yc nu" (NOG) mice, as well as, the use of mouse models for endometriosis. The Core will be responsible for the execution of these complicated techniques for the analysis of endometrial biology and will only generate new models as deemed necessary. The Core will serve Center Investigators by accomplishing the following tasks: 1. The Animal Core will provide the phenotypic analysis of the impact of specific genetic alterations on mouse reproductive biology. 2. The Animal Core will conduct xenograft procedures involved in the reconstitution of endometrial tissue into mice. 3. The Animal Core will manage the colonies of genetically engineered mice for Center Investigators. 4. The Animal Core will produce mutant mice by microinjecting genetically engineered Embryonic Stem cells into the blastocoel of the embryonic day 3.5 mouse embryos and transgenic mice by DNA microinjection into the male pronucleus of one-cell mouse embryos.

动物核心将作为一个资源，为中心的调查人员提供必要的动物和 动物研究技术，以有效地执行他们的研究。动物核心将负责 购买和盘点中心调查人员使用的动物，以及为调查人员提供 操纵各种动物模型所需的技术能力。在过去，动物核心是 对基因工程小鼠的产生负责。然而，该中心的研究重点是 将应用于研究已经建立的小鼠模型的子宫内膜生物学。因此， 这个核心在更新中的重点将是作为核心中使用的动物的储存库和 遗传改变对小鼠生殖生物学影响的表型分析。这一核心将 人子宫内膜组织异种移植在NOD/SCID/YC中的应用 NU“(NOG)小鼠也是如此 采用小鼠子宫内膜异位症模型。核心将负责执行这些 分析子宫内膜生物学的复杂技术，只会产生被认为是新的模型 这是必要的。中心将通过完成以下任务为中心调查人员提供服务： 1.动物核心将提供特定基因改变对小鼠影响的表型分析 生殖生物学。 2.动物核心将进行异种移植程序，将子宫内膜组织重建为 老鼠。 3.动物核心中心将为中心调查人员管理转基因小鼠的群体。 4.动物核心将通过显微注射基因工程胚胎干细胞产生突变小鼠 将DNA显微注射入胚胎3.5天小鼠胚胎和转基因小鼠的胚泡中 小鼠单细胞胚胎的雄性原核。