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CORE B - ANIMAL CORE

核心 B - 动物核心

基本信息

批准号：
7683522
负责人：
Francesco John DeMayo
金额：
$ 11.52万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

The Animal Core will serve as a resource to supply Center investigators with the necessary animals and animal research techniques to effectively execute their research. The Animal Core will be responsible for the purchasing and inventory of the animals used by Center investigators, as well as, providing investigators with the technical ability required in the manipulation of the various animal models. In the past the Animal Core was responsible for the generation of genetically engineered mice. However, the focus of the research in this center application will be on investigation of endometrial biology in mouse models already generated. Therefore, the emphasis of this Core in the renewal will be to serve as a repository for the animals used in the core and for the phenotypic analysis of impact of the genetic alterations on mouse reproductive biology. This Core will incorporate the use of xenotransplants of human endometrial tissue into NOD/SCID/yc nu" (NOG) mice, as well as, the use of mouse models for endometriosis. The Core will be responsible for the execution of these complicated techniques for the analysis of endometrial biology and will only generate new models as deemed necessary. The Core will serve Center Investigators by accomplishing the following tasks: 1. The Animal Core will provide the phenotypic analysis of the impact of specific genetic alterations on mouse reproductive biology. 2. The Animal Core will conduct xenograft procedures involved in the reconstitution of endometrial tissue into mice. 3. The Animal Core will manage the colonies of genetically engineered mice for Center Investigators. 4. The Animal Core will produce mutant mice by microinjecting genetically engineered Embryonic Stem cells into the blastocoel of the embryonic day 3.5 mouse embryos and transgenic mice by DNA microinjection into the male pronucleus of one-cell mouse embryos.

动物核心将作为向中心研究者提供必要动物的资源，动物研究技术，以有效地执行他们的研究。动物中心将负责中心研究者使用的动物的采购和库存，以及向研究者提供操作各种动物模型所需的技术能力。在过去，动物的核心是负责基因工程小鼠的产生。然而，该中心的研究重点是将应用于已经产生的小鼠模型中子宫内膜生物学的研究。因此在更新过程中，该核心的重点将是作为核心中使用的动物的储存库，遗传改变对小鼠生殖生物学影响的表型分析。这一核心将将人子宫内膜组织异种移植物的使用并入NOD/SCID/yc nu”（NOG）小鼠，以及如子宫内膜异位症小鼠模型的使用。核心将负责执行这些用于子宫内膜生物学分析的复杂技术，并且只会产生新的模型，必要核心人员将通过完成以下任务为中心研究者提供服务： 1.动物核心将提供特定遗传改变对小鼠的影响的表型分析。生殖生物学 2.动物中心将进行涉及子宫内膜组织重建的异种移植手术，小鼠 3.动物中心将为中心研究人员管理基因工程小鼠的菌落。 4.动物核心将通过显微注射基因工程胚胎干细胞产生突变小鼠 5天小鼠胚胎的囊胚腔中，并通过DNA显微注射到单细胞小鼠胚胎的雄性原核。