GENETICALLY ENGINEERED MOUSE

基因工程鼠标

• 批准号: 8180968
• 负责人: Francesco John DeMayo
• 金额: $ 11.05万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-17 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180968
• 关键词: Adopted; Animal Experimentation; Animal Model; Animals; Cancer Center; Cancer Etiology; Cells; Cryopreservation; DNA; Development; Diagnostic; Embryo; Embryo Transfer; Engineering; Ensure; Equipment; Generations; Genetically Engineered Mouse; Injection of therapeutic agent; Investigation; Knockout Mice; Medicine; Microinjections; Molecular and Cellular Biology; Mus; Mutant Strains Mice; Names; Preclinical Testing; Production; Reagent; Recording of previous events; Research; Research Personnel; Resource Sharing; Resources; Schools; Services; Source; Superovulation; System; Technical Expertise; Techniques; Training; Transgenic Mice; animal resource; cancer therapy; college; cost; design; embryonic stem cell; experimental analysis; improved; in vivo; mouse model; preclinical evaluation; repository; reproductive; research study; tumor progression

The objectives of the Genetically Engineered Mouse (GEM) shared resource is to aid Cancer Center investigators with the design and execution of experiments to utilize animal models for the investigation of the progression of cancer and in the preclinical evaluation of therapies for the treatment of cancer. Although the use of animal models provides an invaluable source of reagents for preclinical testing and the investigation of regulatory mechanisms in vivo, in a physiologically regulated system, the use of animals is costly with respect to equipment, animal resources and technical expertise. GEM will serve as a resource for the execution and training in the use of all animal manipulations needed in the Cancer Center. The GEM shared resource will minimize the cost of utilizing animal models in the Cancer Center by consolidating animal expertise and associated equipment. This will ensure an efficient use of animal research at minimal cost. The GEM shared resource will serve Cancer Center investigatory by aiding in the following tasks. 1. Generate transgenic mice by the micro injection of DNA into the one celled mouse embryo. 2. Generate mutant mice by the manipulation of mouse Embryonic Stem Cells. 3. Preserve lines of mice by cryopreservation. 4 Assist investigators with the importation and exporting of lines of mice. The accomplishments of these tasks will allow the GEM Shared Resource to serve as a repository for all genetically engineered mouse models and techniques for Cancer Center investigators and will ensure efficient use of animal resources by Cancer Center investigators.

基因工程小鼠(GEM)共享资源的目标是帮助癌症中心的研究人员设计和执行实验，以利用动物模型研究癌症的进展情况，并在癌症治疗的临床前评估中使用。尽管动物模型的使用为临床前试验和体内调控机制的研究提供了宝贵的试剂来源，但在生理调节系统中，使用动物在设备、动物资源和技术专业知识方面是昂贵的。GEM将作为一个资源，用于执行和培训癌症中心所需的所有动物操作。GEM共享资源将通过整合动物专业知识和相关设备，将癌症中心使用动物模型的成本降至最低。这将确保以最低的成本有效地使用动物研究。GEM共享资源将通过协助完成以下任务为癌症中心研究人员提供服务。1.将DNA显微注射到单细胞小鼠胚胎中，获得转基因小鼠。2.通过操纵小鼠胚胎干细胞获得突变小鼠。3.采用超低温冻存法保存小鼠品系。4协助调查人员进行品系老鼠的进出口工作。这些任务的完成将使GEM共享资源成为癌症中心研究人员所有转基因小鼠模型和技术的储存库，并将确保癌症中心研究人员有效利用动物资源。