Genetically Engineered Mouse Resource

基因工程小鼠资源

• 批准号: 7514629
• 负责人: Francesco John DeMayo
• 金额: $ 8.39万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7514629
• 关键词: Adopted; Animal Experimentation; Animal Model; Animals; Area; Cancer Center; Cells; Cryopreservation; DNA; Derivation procedure; Embryo; Embryo Transfer; Engineering; Ensure; Equipment; Generations; Genetically Engineered Mouse; Injection of therapeutic agent; Investigation; Knockout Mice; Medicine; Microinjections; Molecular and Cellular Biology; Mus; Mutant Strains Mice; Names; Preclinical Testing; Production; Purpose; Reagent; Recording of previous events; Research; Research Personnel; Resource Sharing; Resources; Schools; Services; Source; Superovulation; System; Technical Expertise; Techniques; Therapy Evaluation; Training; Transgenic Mice; animal resource; cancer therapy; college; cost; design; embryonic stem cell; experimental analysis; in vivo; mouse model; pre-clinical; repository; reproductive; research study; tumor progression

The objective of the Genetically Engineered Mouse (GEM) Resource is to aid Cancer Center investigators with the design and execution of experiments to utilize animal models for the investigation of the progression of cancer and for the preclinical evaluation of therapies for the treatment of cancer. Although the use of animal models provides an invaluable source of reagents for preclinical testing and the investigation of regulatory mechanisms in vivo in a physiologically regulated system, the use of animals is costly with respect to equipment, animal resources, and technical expertise. The GEM Core will serve as a resource for carrying out and training in the use of all animal manipulations needed in the Cancer Center. The GEM Core will minimize the cost of utilizing animal models in the Cancer Center by consolidating the equipment, expertise, and animal resources. This will ensure an efficient use of animal research at minimal cost. The GEM Core will serve Cancer Center investigators by aiding in the following tasks. 1. The GEM Core will generate transgenic mice by the micro injection of DMA into one-celled mouse embryos. 2. The GEM Core will generate mutant mice by the manipulation of mouse Embryonic Stem Cells. 3. The Core will preserve lines of mice by cryopreservation. 4 The Core will aid investigators with the importation and exporting of lines of mice. The accomplishments of these tasks will allow the GEM Core to serve as a repository for all genetically engineered mouse models and techniques for Cancer Center investigators and will ensure efficient use of animal resources by Cancer Center investigators. The Shared Resource Leader is Dr. Francesco DeMayo, who has an extensive track record in these areas

基因工程小鼠（GEM）资源的目的是帮助癌症中心的研究人员 通过设计和执行实验，利用动物模型研究进展， 以及用于治疗癌症的疗法的临床前评价。虽然使用 动物模型为临床前试验和研究提供了宝贵的试剂来源， 由于在生理调节系统中的体内调节机制，使用动物在这方面是昂贵的， 设备、动物资源和技术专长。GEM核心将作为以下方面的资源： 在癌症中心进行和培训使用所有需要的动物操作。GEM核心 通过整合设备，将癌症中心使用动物模型的成本降到最低， 专业知识和动物资源。这将确保以最低成本有效利用动物研究。 GEM Core将通过协助以下任务为癌症中心研究人员提供服务。 1. GEM Core将通过将DMA显微注射到单细胞小鼠中来产生转基因小鼠 胚胎 2. GEM Core将通过操作小鼠胚胎干细胞产生突变小鼠。 3.核心将通过冷冻保存保存小鼠品系。 4核心将帮助研究人员进口和出口小鼠品系。 这些任务的完成将使GEM核心成为所有基因的储存库， 为癌症中心研究人员设计的小鼠模型和技术，并将确保有效利用 动物资源癌症中心的研究人员。共享资源负责人是Francesco DeMayo博士， 他在这些领域有着广泛的记录