Gene Vector Virus Core

基因载体病毒核心

• 批准号: 9201333
• 负责人: Javier Alcudia
• 金额: $ 16.95万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9201333
• 关键词: Aging; Brain; CRISPR/Cas technology; Canine Adenoviruses; Cells; Chromatography; Communities; Custom; Development; Disease Progression; Equipment; Experimental Designs; Faculty; Funding; Gene Delivery; Generations; Genes; Genetic; Grant; Institutes; Laboratories; Learning; Life Experience; Light; Location; Logistics; Maintenance; Microscopy; Molecular; Nervous system structure; Neurons; Neurosciences; Neurosciences Research; Plasmids; Preparation; Process; Production; Proteins; Recombinant Proteins; Reporting; Research; Research Activity; Research Personnel; Roller Bottle; Running; Services; Slice; Source; Specificity; Stem cells; Surveys; System; Techniques; Technology; Transportation; Viral; Viral Vector; Virus; experimental study; fast protein liquid chromatography; genetic manipulation; genome editing; innovation; interest; medical schools; meetings; new technology; optogenetics; promoter; repository; scale up; tool; vector; virus core

Project Summary (GVVC) As one of three Stanford Neuroscience Cores, the Gene Vector and Virus Core (GVVC) provides viral vectors to manipulate and express recombinant proteins in the whole brain, in slices, and in cellular systems in a temporally and spatially controlled manner. The ability to employ these molecular techniques is essential to the daily research activities of a significant number of neuroscience labs at Stanford, and with the continued development of new molecular tools to report on and to manipulate neuronal function, and to trace neuronal connections with greater specificity, these techniques will remain critical to advancing our progress in understanding brain function. Over the past 4 years the GVVC has made 1348 viruses and provided 219 pre- made stock viruses for 581 projects. Moreover, GVVC has supported the viral production for the innovative optogenetics technology, in which light-sensitive channels (channelrhodopsins) are used to regulate neuronal activity, invented by the Dr. Karl Deisseroth laboratory. Logistical proximity of GVVC and its support for Deisseroth laboratory has led to dissemination of this technology not only to Stanford faculty but to many other neuroscientists nationwide. Since its inception, GVVC has continued to add to the number of stock viruses in its virus repository (http://med.stanford.edu/gvvc/stockviruses.html) which is accessible for distribution. Under the proposed grant, GVVC will continue to provide its present cutting-edge services. In addition, GVVC will develop new services, such as CAV-2 (canine adenovirus) virus, that will allow retrograde axonal transportation.

项目概要（GVVC） 作为斯坦福大学三个神经科学核心之一，基因载体和病毒核心（GVVC）提供病毒 在全脑、切片和细胞系统中操纵和表达重组蛋白的载体， 时间和空间控制的方式。使用这些分子技术的能力对于 斯坦福大学大量神经科学实验室的日常研究活动， 开发新的分子工具来报告和操纵神经元功能，并追踪神经元 更具体的联系，这些技术将仍然是至关重要的，以推动我们的进展， 了解大脑功能。在过去的4年里，GVVC已经制造了1348种病毒，并提供了219种前病毒。 为581个项目制作了库存病毒。此外，GVVC还支持创新的病毒生产， 光遗传学技术，其中光敏通道（通道视紫红质）用于调节神经元 由卡尔·戴瑟罗斯博士实验室发明。GVVC的对数接近度及其对 Deisseroth实验室不仅将这项技术传播给了斯坦福大学的教职员工， 全国神经科学家自成立以来，GVVC不断增加库存病毒的数量， 其病毒库（http：//med.stanford.edu/gvvc/stockviruses.html），可供分发。下 GVVC将继续提供其现有的尖端服务。此外，GVVC将 开发新的服务，如CAV-2（犬腺病毒）病毒，这将允许逆行轴突 运输