Measurement of protein associations via static light scattering
通过静态光散射测量蛋白质关联
基本信息
- 批准号:7593447
- 负责人:
- 金额:$ 16.84万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:Annual ReportsComputer softwareConditionCooperative Research and Development AgreementDataData AnalysesEquilibriumFutureHourIndividualLaboratoriesMeasurementModelingProductionProteinsPumpSyringesSystemTechniquesTechnologyTestingTimeValidationWorkbaseconceptdata acquisitiondesigndetectorlight scatteringnephelometrynovelresearch study
项目摘要
We have been testing pre-production versions of a system designed by Wyatt Technology Incorporated to facilitate composition-gradient static light scattering measurements. The system, based upon a concept developed previously in our laboratory (see annual reports of the previous two years), consists of a pump module containing three syringe pumps and an inline degasser, and a software package that is used to control the syringes to create composition gradients, to collect data from light scattering and concentration detectors, and to analyze the data in the context of models for equilibrium self-association of a single protein and hetero-association of two proteins. The hardware and software were loaned to us by Wyatt Technology under terms of CRADA DK-05-0104. Preliminary results indicate that with this system one can characterize and quantify equilibrium self-associations of each of two individual protein species and hetero-associations of the two proteins in a single experiment requiring only about 2-3 mg of each protein and less than one hour of data acquisition time.
我们一直在测试由Wyatt Technology Incorporated设计的系统的预生产版本,以促进成分梯度静态光散射测量。 该系统基于我们实验室以前开发的概念,(参见前两年的年度报告),由一个泵模块和一个软件包组成,泵模块包含三个注射泵和一个在线脱气器,软件包用于控制注射器以创建成分梯度,从光散射和浓度检测器收集数据,并在单个蛋白质的平衡自缔合和两个蛋白质的异质缔合的模型的背景下分析数据。 硬件和软件由Wyatt Technology根据CRADA DK-05-0104条款借给我们。 初步结果表明,利用该系统,可以在单个实验中表征和量化两种单独蛋白质种类中的每一种的平衡自缔合和两种蛋白质的异质缔合,所述单个实验仅需要约2-3 mg的每种蛋白质和少于一小时的数据采集时间。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Allen P Minton其他文献
Proton nuclear magnetic resonance studies of hemoglobin M Milwaukee and their implications concerning the mechanism of cooperative oxygenation of hemoglobin.
血红蛋白 M Milwaukee 的质子核磁共振研究及其对血红蛋白协同氧化机制的影响。
- DOI:
10.1021/bi00626a033 - 发表时间:
1977 - 期刊:
- 影响因子:2.9
- 作者:
Leslie W.;Allen P Minton;Ted R. Lindstrom;Anthony V. Pisciotta;Chien Ho - 通讯作者:
Chien Ho
Synexin (annexin VII) hypothesis for Ca2+/GTP-regulated exocytosis.
Ca2 /GTP 调节的胞吐作用的 Synexin (annexin VII) 假说。
- DOI:
10.1016/s1054-3589(08)60701-2 - 发表时间:
1998 - 期刊:
- 影响因子:0
- 作者:
Harvey B. Pollard;H. Caohuy;H. Caohuy;Allen P Minton;M. Srivastava;M. Srivastava - 通讯作者:
M. Srivastava
The bivalent ligand hypothesis. A quantitative model for hormone action.
二价配体假说。
- DOI:
- 发表时间:
1981 - 期刊:
- 影响因子:3.6
- 作者:
Allen P Minton - 通讯作者:
Allen P Minton
Holobiochemistry: the effect of local environment upon the equilibria and rates of biochemical reactions.
- DOI:
10.1016/0020-711x(90)90102-9 - 发表时间:
1990 - 期刊:
- 影响因子:0
- 作者:
Allen P Minton - 通讯作者:
Allen P Minton
Simplified Equilibrium Model for Exploring the Combined Influences of Concentration, Aggregate Shape, Excluded Volume, and Surface Adsorption upon Aggregation Propensity and Distribution of Globular Macromolecules.
用于探索浓度、聚集体形状、排除体积和表面吸附对球状大分子聚集倾向和分布的综合影响的简化平衡模型。
- DOI:
10.1021/acs.jpcb.3c05594 - 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Allen P Minton - 通讯作者:
Allen P Minton
Allen P Minton的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Allen P Minton', 18)}}的其他基金
Noncovalent Intermolecular Interactions In Biochemistry
生物化学中的非共价分子间相互作用
- 批准号:
6809901 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
NONCOVALENT INTERMOLECULAR INTERACTIONS IN BIOCHEMISTRY
生物化学中的非共价分子间相互作用
- 批准号:
6432066 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
Thermodynamic and kinetic studies of macromolec structure and enzymic mechanisms
大分子结构和酶机制的热力学和动力学研究
- 批准号:
8553397 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
Measurement of biomolecular association via static and dynamic light scattering
通过静态和动态光散射测量生物分子缔合
- 批准号:
8148691 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
Thermodynamic and kinetic studies of protein structure and enzymic mechanisms
蛋白质结构和酶机制的热力学和动力学研究
- 批准号:
8148698 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
NONCOVALENT INTERMOLECULAR INTERACTIONS IN BIOCHEMISTRY
生物化学中的非共价分子间相互作用
- 批准号:
6289725 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
Noncovalent Intermolecular Interactions In Biochemistry
生物化学中的非共价分子间相互作用
- 批准号:
6507261 - 财政年份:
- 资助金额:
$ 16.84万 - 项目类别:
相似海外基金
Expanding the Nation’s STEM Talent Pool by Accelerating Graduate Degree Completion in Computer, Software, and Cybersecurity Engineering
通过加快计算机、软件和网络安全工程研究生学位的完成来扩大国家的 STEM 人才库
- 批准号:
2221602 - 财政年份:2022
- 资助金额:
$ 16.84万 - 项目类别:
Standard Grant
Radio telescope computer software for detecting exotic astronomical sources that change with time
用于探测随时间变化的奇异天文源的射电望远镜计算机软件
- 批准号:
1615884 - 财政年份:2016
- 资助金额:
$ 16.84万 - 项目类别:
Standard Grant
Radio telescope computer software for detecting exotic astronomical sources that change with time
用于探测随时间变化的奇异天文源的射电望远镜计算机软件
- 批准号:
1615342 - 财政年份:2016
- 资助金额:
$ 16.84万 - 项目类别:
Standard Grant
Computer Software to Track Roll-Over Stability of Tractor-Agri-Machine Systems
用于跟踪拖拉机农机系统侧翻稳定性的计算机软件
- 批准号:
479955-2015 - 财政年份:2015
- 资助金额:
$ 16.84万 - 项目类别:
University Undergraduate Student Research Awards
Computer Software for Reconstruction and Retrospective Assessment of Interim Decisions and their Rules in Randomized Controlled Trials
用于随机对照试验中临时决策及其规则的重建和回顾性评估的计算机软件
- 批准号:
297265 - 财政年份:2013
- 资助金额:
$ 16.84万 - 项目类别:
Fellowship Programs
Effects of cognitive rehabilitation using original computer software in schizophrenia: a randomized controlled trial
使用原始计算机软件对精神分裂症进行认知康复的效果:一项随机对照试验
- 批准号:
25861024 - 财政年份:2013
- 资助金额:
$ 16.84万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of new program for agricultural education by using the computer software Virtual Field System and the many movies recording plant growth
利用计算机软件Virtual Field System和记录植物生长的许多电影开发新的农业教育程序
- 批准号:
21500864 - 财政年份:2009
- 资助金额:
$ 16.84万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
NSF Travel Grant Support for IEEE Computer Software and Applications Conference (COMPSAC) 2009
NSF 为 2009 年 IEEE 计算机软件和应用程序会议 (COMPSAC) 提供差旅补助金支持
- 批准号:
0850818 - 财政年份:2008
- 资助金额:
$ 16.84万 - 项目类别:
Standard Grant
Computer software for calculations in particle physics
用于粒子物理计算的计算机软件
- 批准号:
326734-2006 - 财政年份:2008
- 资助金额:
$ 16.84万 - 项目类别:
Subatomic Physics Envelope - Individual
Computer software for calculations in particle physics
用于粒子物理计算的计算机软件
- 批准号:
326734-2006 - 财政年份:2007
- 资助金额:
$ 16.84万 - 项目类别:
Subatomic Physics Envelope - Individual