Use of systems pharmacology to prevent rod and cone photoreceptor degeneration
利用系统药理学预防视杆细胞和视锥细胞光感受器变性
基本信息
- 批准号:9554184
- 负责人:
- 金额:$ 42万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-07-01 至 2018-03-31
- 项目状态:已结题
- 来源:
- 关键词:AblationAdenylate CyclaseAffectAge related macular degenerationAgonistAnimal ModelAntibodiesAvastinBasic ScienceBiochemical ProcessBlindnessCanis familiarisCell DeathCell SurvivalCellsClinicClinical TreatmentClinical TrialsCombined Modality TherapyComplexConeCyclic AMPDataDevelopmentDiseaseDoseDrug IndustryDrug KineticsDrug effect disorderDysplasiaEffectivenessEngineeringEnsureEvaluationEventExhibitsExudative age-related macular degenerationEyeFDA approvedFoundationsFutureG-Protein-Coupled ReceptorsGene ExpressionGeneticGenetic TranscriptionGoalsHumanInstitutesInterventionKnock-in MouseKnock-outLaboratoriesLightLinkLucentisLysosomesMedicalMedical centerMessenger RNAMichiganMicroRNAsModelingMonitorMonoclonal AntibodiesMonoclonal Antibody R24MusNational Eye InstituteOcular PathologyOphthalmologyOutcomePartner in relationshipPathologyPathway interactionsPharmaceutical PreparationsPharmacodynamicsPharmacological TreatmentPharmacologyPharmacotherapyPhotoreceptorsPhysiologyProductionPropertyProteinsResearchResearch PersonnelRetinaRetinalRetinal ConeRetinal DegenerationRetinal DiseasesRetinitis PigmentosaSafetySecond Messenger SystemsSecondary toSignal PathwaySignal TransductionSiteSpecificityStargardt&aposs diseaseStructureStructure of retinal pigment epitheliumSystemTechnologyTestingTherapeuticTherapeutic AgentsTherapeutic InterventionTherapeutic Monoclonal AntibodiesTherapeutic antibodiesToxic effectTranslatingTreatment EfficacyUniversitiesUntranslated RNAValidationVertebrate PhotoreceptorsVision DisordersVisual impairmentVisual system structureWashingtonanalytical methodantagonist Gbasebevacizumabbiological systemscombatdesigndrug candidateenvironmental stressorflexibilityhigh resolution imagingimaging modalityimprovedinterestmouse modelnext generation sequencingnovelnovel strategiesoverexpressionphotoreceptor degenerationpreventprotective effectranibizumabreceptorresearch clinical testingrestorationretinal damageretinal rodsscreeningsmall moleculesuccesssuccessful interventiontargeted treatmenttranscriptometranslational research program
项目摘要
ABSTRACT
An interdisciplinary consortium of investigators from the Departments of Pharmacology and Ophthalmology
at Case Western Reserve University, the Cleveland Louis Stokes VA Medical Center, the Cole Eye Institute at
Cleveland Clinic Foundation, Washington University, Michigan State University, Johns Hopkins University and
ingenious Targeting Laboratory, Inc., proposes "to increase the pace at which basic science discoveries of
disease mechanisms can be translated into therapies for complex visual system disorders and disease", a goal
of the R24 National Eye Institute Translational Research Program on Therapy for Visual Disorders. This scientific
partnership will employ its diverse expertise to evaluate potential therapies for retinal diseases in animal models
by using a cutting-edge systems pharmacology paradigm. By screening a combination of G protein-coupled
receptor (GPCR) agonist/antagonist drugs (modulators) for their ability to prevent retinal pathology in animal
models of various rod and cone photoreceptor cell retinopathies, we will identify suitable candidates for future
testing in humans. High resolution imaging methods and transcriptional analysis among other approaches will
be used to monitor the efficacy and safety of these combination therapies. The goals of this project are: Aim 1.
Test in mice the efficacy of a combination of GPCR agonists/antagonists in protecting against light-induced
retinal damage and with either spontaneous rapid or slowly-progressing cone degeneration. Aim 2.
Pharmacologically and genetically validate the involvement and specificity of identified GPCRs and their precise
subcellular localization using knockin mice with T4 lysosome (T4L) fused into their signaling domains and an
antibody we developed against T4L. We also will use the FAST system that allows various genetic outcomes
(from inactivation to overexpression) to be engineered in a single mouse model. Aim 3. Assess the effectiveness
of GPCR therapy in a canine rod-cone retinopathy model. Aim 4. Expand the range of receptor modulators to
include therapeutic antibodies. Fulfilling these interrelated aims will direct our development of more successful
therapies for people with incurable blinding diseases.
摘要
由药理学和眼科的研究人员组成的跨学科联盟
在凯斯西储大学,克利夫兰路易斯·斯托克斯退伍军人医学中心,科尔眼科研究所
克利夫兰临床基金会、华盛顿大学、密歇根州立大学、约翰霍普金斯大学和
独具匠心的靶向实验室公司提出,“加快基础科学发现的步伐
疾病机制可转化为复杂视觉系统障碍和疾病的治疗“,一个目标
R24国家眼科研究所视力障碍治疗的翻译研究项目。这是科学的
合作伙伴公司将利用其不同的专业知识在动物模型中评估视网膜疾病的潜在治疗方法
通过使用尖端系统药理学范例。通过筛选与G蛋白偶联的组合
受体(GPCR)激动剂/拮抗剂药物(调节剂)预防动物视网膜病变的能力
各种视杆细胞和视锥细胞视网膜病变的模型,我们将为未来确定合适的候选模型
在人体上进行测试。高分辨率成像方法和转录分析等方法将
用于监测这些联合疗法的有效性和安全性。本项目的目标是:目标1。
GPCR激动剂/拮抗剂联合应用对光诱导小鼠的保护作用
视网膜损伤,并伴有自发性的快速或缓慢的视锥细胞变性。目标2.
从药理学和遗传学上验证已识别的GPCRs及其精确的参与和特异性
T4溶酶体(T4L)融合到其信号域和An的敲击小鼠的亚细胞定位
我们开发的针对T4L的抗体。我们还将使用FAST系统,允许各种遗传结果
(从失活到过度表达)将在单个小鼠模型中进行工程设计。目标3.评估有效性
在犬视杆-视锥视网膜病变模型中应用gpr治疗。目标4.将受体调节剂的范围扩大到
包括治疗性抗体。实现这些相互关联的目标将指导我们的发展取得更大成功
为患有不治之症的人提供治疗方法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Krzysztof Palczewski其他文献
Krzysztof Palczewski的其他文献
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{{ truncateString('Krzysztof Palczewski', 18)}}的其他基金
Precision genome editing in vivo to treat retinal diseases
体内精准基因组编辑治疗视网膜疾病
- 批准号:
10565189 - 财政年份:2023
- 资助金额:
$ 42万 - 项目类别:
The complex role of phosphodiesterase 6 in rod photoreceptor health and function
磷酸二酯酶 6 在视杆光感受器健康和功能中的复杂作用
- 批准号:
10662478 - 财政年份:2020
- 资助金额:
$ 42万 - 项目类别:
The complex role of phosphodiesterase 6 in rod photoreceptor health and function
磷酸二酯酶 6 在视杆光感受器健康和功能中的复杂作用
- 批准号:
10455528 - 财政年份:2020
- 资助金额:
$ 42万 - 项目类别:
A two-photon ophthalmoscope for human retinal imaging and functional testing
用于人类视网膜成像和功能测试的双光子检眼镜
- 批准号:
9059094 - 财政年份:2015
- 资助金额:
$ 42万 - 项目类别:
Regulation of Retinal Physiology by micro-RNAs
micro-RNA 对视网膜生理学的调节
- 批准号:
8627170 - 财政年份:2013
- 资助金额:
$ 42万 - 项目类别:
Regulation of Retinal Physiology by micro-RNAs
micro-RNA 对视网膜生理学的调节
- 批准号:
8431587 - 财政年份:2013
- 资助金额:
$ 42万 - 项目类别:
Photoreceptor Renewal by Retinal Pigmented Epithelium Phagocytosis
视网膜色素上皮吞噬作用的光感受器更新
- 批准号:
8700414 - 财政年份:2012
- 资助金额:
$ 42万 - 项目类别:
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