权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Imaging and Media Core

分子成像和媒体核心

基本信息

批准号：
9360971
负责人：
Alexander Martinus Van der Linden
金额：
$ 7.43万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9360971
关键词：
Alpha Cell Animal Model Basic Science Biology Businesses Caenorhabditis elegans Cells Cellular biology Centers of Research Excellence Collaborations Contract Services Core Facility Coupled DNA DNA analysis Detection Drosophila genus Dyes Educational workshop Equipment Faculty Fee-for-Service Plans Fees Food Fostering Future Genes Goals Image Imaging Device Imaging Techniques Individual Invertebrates Isotope Labeling Maintenance Microscope Microscopy Modeling Molecular Molecular Biology Neurons Neurosciences Neurosciences Research Phase Population Postdoctoral Fellow Preparation Protein Analysis Proteins RNA RNA analysis Radio Research Research Infrastructure Research Personnel Research Project Grants Research Support Resolution Resource Sharing Resources Sampling Structure Students Technical Expertise Techniques Tilia Time Training Training Support Transcript Work cost digital imaging fly food preparation graduate student imaging system improved instrument investigator training laser capture microdissection medical schools microscopic imaging molecular imaging single cell analysis

项目摘要

PROJECT SUMMARY/ABSTRACT Molecular Imaging and Media Core The Molecular Imaging and Media and Microscopy (Core D) was established in the 4th year of Phase I, and has directly benefited two COBRE project investigators and other faculty and students using basic molecular approaches at UNR. The core will be directed and managed by Dr. Alexander van der Linden (graduated COBRE project leader), and will provide infrastructure, training and equipment required for the high quality analysis of biomolecules, media and sample preparation resources, and microscopy. The core houses a Biorad V3 Chemidoc and a GE Typhoon FLA7000 IP imager that allow high-resolution digital imaging of DNA, RNA, protein and dyes by means of, for instance, chemiluminescence and radio isotopic labels. The core also houses two Biorad CFX96 Real-Time PCRs for detection and quantification of gene transcript levels. In addition, the core houses an Integra Mediaclave 10 coupled to a dispenser and other accessories that allow preparation of food and media for Drosophila and C. elegans research. The main goal of this core in phase II is to further enhance the scope and quality of basic research by providing workshops, training and technical expertise of core instruments to users. The core in phase II will also establish a new centralized facility for husbandry and maintenance of Drosophila, which will directly promote the research of 5 research groups including two COBRE project leaders. The core in phase II will be expanded to provide training and support to a shared confocal microscope in the Biology Department, which will be co-managed by the Neuro COBRE and Cell Biology COBRE. Moreover, as part of an institutional commitment, the core in phase II will be expanded with a Laser Capture Microdissection scope for isolating specific cells from heterogenous cell populations, allowing downstream single-cell analysis of DNA, RNA and protein. Taken together, the core will directly support the aims of two COBRE projects and enhance the scope and quality of 7 additional research groups within the Biology Department as well as other UNR investigators.

项目概要/摘要分子成像和媒体核心分子成像与介质和显微镜（核心D）于第一阶段的第四年建立，并且使用基础分子技术使两名 COBRE 项目研究人员和其他教职员工和学生直接受益 UNR 的方法。该核心将由 Alexander van der Linden 博士（毕业于 COBRE 项目负责人），并将提供高质量所需的基础设施、培训和设备生物分子、介质和样品制备资源以及显微镜分析。核心内装有一个 Biorad V3 Chemidoc 和 GE Typhoon FLA7000 IP 成像仪可实现 DNA 的高分辨率数字成像， RNA、蛋白质和染料，例如通过化学发光和放射性同位素标记。核心还配备两台 Biorad CFX96 实时 PCR，用于基因转录水平的检测和定量。在此外，核心还装有一个 Integra Mediaclave 10，该 Integra Mediaclave 10 与分配器和其他配件相连，可让为果蝇和线虫研究准备食物和培养基。该核心第二阶段的主要目标是通过提供讲习班、培训和技术，进一步扩大基础研究的范围和质量向用户提供核心仪器的专业知识。第二阶段的核心还将建立一个新的集中设施果蝇饲养与维护，将直接推动5个课题组的研究其中包括两名 COBRE 项目负责人。第二阶段的核心将得到扩展，以提供培训和支持生物系共用一台共聚焦显微镜，由 Neuro COBRE 和细胞生物学 COBRE。此外，作为机构承诺的一部分，第二阶段的核心将得到扩展具有激光捕获显微切割范围，用于从异质细胞群中分离特定细胞，允许对 DNA、RNA 和蛋白质进行下游单细胞分析。综合起来，核心将直接支持两个 COBRE 项目的目标，并提高 7 个额外研究小组的范围和质量生物系以及其他 UNR 调查员。