Molecular Imaging and Media Core

项目摘要/摘要 分子成像与媒体核心 分子成像和媒体与显微镜(核心D)成立于第一阶段的第四年，以及 使两名Cobre项目研究人员和其他使用Basic分子的教职员工和学生直接受益 在UNR的方法。核心将由Alexander van der Linden博士(毕业)指导和管理 科布雷项目负责人)，并将提供高质量所需的基础设施、培训和设备 分析生物分子、介质和样品制备资源，以及显微镜。核心容纳了一个 Biorad V3 Chemidoc和通用电气台风FLA7000 IP成像仪，允许对DNA进行高分辨率数字成像， RNA、蛋白质和染料，例如通过化学发光和放射性同位素标记。核心还包括 拥有两个Biorad CFX96实时PCR，用于检测和量化基因转录水平。在……里面 此外，核心包含一个集成Mediaclave 10连接到一个分配器和其他附件，允许 果蝇和线虫研究的食物和介质的制备。该核心在第二阶段的主要目标是 通过举办讲习班、培训和技术培训，进一步提高基础研究的范围和质量 将核心仪器的专业知识带给用户。第二阶段的核心还将建立一个新的中央设施，用于 果蝇的饲养和维护，将直接推动5个课题组的研究 包括两名科布雷项目负责人。第二阶段的核心将扩大，以提供培训和支助 生物部共用共焦显微镜，将由Neuro Cobre和 细胞生物学科布雷。此外，作为机构承诺的一部分，第二阶段的核心将扩大。 利用激光捕获显微切割显微镜从异种细胞群中分离特定细胞， 允许对DNA、RNA和蛋白质进行下游单细胞分析。加在一起，核心将直接 支持两个Cobre项目的目标，并加强另外7个研究小组的范围和质量 生物系内部以及其他联合国资源研究所的调查人员。

PROJECT SUMMARY/ABSTRACT Molecular Imaging and Media Core The Molecular Imaging and Media and Microscopy (Core D) was established in the 4th year of Phase I, and has directly benefited two COBRE project investigators and other faculty and students using basic molecular approaches at UNR. The core will be directed and managed by Dr. Alexander van der Linden (graduated COBRE project leader), and will provide infrastructure, training and equipment required for the high quality analysis of biomolecules, media and sample preparation resources, and microscopy. The core houses a Biorad V3 Chemidoc and a GE Typhoon FLA7000 IP imager that allow high-resolution digital imaging of DNA, RNA, protein and dyes by means of, for instance, chemiluminescence and radio isotopic labels. The core also houses two Biorad CFX96 Real-Time PCRs for detection and quantification of gene transcript levels. In addition, the core houses an Integra Mediaclave 10 coupled to a dispenser and other accessories that allow preparation of food and media for Drosophila and C. elegans research. The main goal of this core in phase II is to further enhance the scope and quality of basic research by providing workshops, training and technical expertise of core instruments to users. The core in phase II will also establish a new centralized facility for husbandry and maintenance of Drosophila, which will directly promote the research of 5 research groups including two COBRE project leaders. The core in phase II will be expanded to provide training and support to a shared confocal microscope in the Biology Department, which will be co-managed by the Neuro COBRE and Cell Biology COBRE. Moreover, as part of an institutional commitment, the core in phase II will be expanded with a Laser Capture Microdissection scope for isolating specific cells from heterogenous cell populations, allowing downstream single-cell analysis of DNA, RNA and protein. Taken together, the core will directly support the aims of two COBRE projects and enhance the scope and quality of 7 additional research groups within the Biology Department as well as other UNR investigators.