Molecular Imaging and Media Core

分子成像和媒体核心

• 批准号: 10194515
• 负责人: Alexander Martinus Van der Linden
• 金额: $ 8.32万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-05 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10194515
• 关键词: Animal Model; Basic Science; Biology; Businesses; Caenorhabditis elegans; Cells; Cellular biology; Centers of Research Excellence; Collaborations; Contract Services; Core Facility; Coupled; DNA; DNA analysis; Detection; Drosophila genus; Dyes; Educational workshop; Equipment; Faculty; Fee-for-Service Plans; Fees; Food; Fostering; Future; Genes; Goals; Image; Imaging Device; Imaging Techniques; Individual; Infrastructure; Invertebrates; Isotope Labeling; Maintenance; Microscope; Microscopy; Modeling; Molecular; Molecular Biology; Neurons; Neurosciences; Neurosciences Research; Phase; Population; Postdoctoral Fellow; Preparation; Proteins; RNA; Radio; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Resource Sharing; Resources; Sampling; Structure; Students; Technical Expertise; Techniques; Tilia; Time; Training; Training Support; Transcript; Work; confocal imaging; cost; digital imaging; fly; food preparation; graduate student; imager; imaging system; improved; instrument; investigator training; laser capture microdissection; medical schools; microscopic imaging; molecular imaging; single cell analysis

PROJECT SUMMARY/ABSTRACT Molecular Imaging and Media Core The Molecular Imaging and Media and Microscopy (Core D) was established in the 4th year of Phase I, and has directly benefited two COBRE project investigators and other faculty and students using basic molecular approaches at UNR. The core will be directed and managed by Dr. Alexander van der Linden (graduated COBRE project leader), and will provide infrastructure, training and equipment required for the high quality analysis of biomolecules, media and sample preparation resources, and microscopy. The core houses a Biorad V3 Chemidoc and a GE Typhoon FLA7000 IP imager that allow high-resolution digital imaging of DNA, RNA, protein and dyes by means of, for instance, chemiluminescence and radio isotopic labels. The core also houses two Biorad CFX96 Real-Time PCRs for detection and quantification of gene transcript levels. In addition, the core houses an Integra Mediaclave 10 coupled to a dispenser and other accessories that allow preparation of food and media for Drosophila and C. elegans research. The main goal of this core in phase II is to further enhance the scope and quality of basic research by providing workshops, training and technical expertise of core instruments to users. The core in phase II will also establish a new centralized facility for husbandry and maintenance of Drosophila, which will directly promote the research of 5 research groups including two COBRE project leaders. The core in phase II will be expanded to provide training and support to a shared confocal microscope in the Biology Department, which will be co-managed by the Neuro COBRE and Cell Biology COBRE. Moreover, as part of an institutional commitment, the core in phase II will be expanded with a Laser Capture Microdissection scope for isolating specific cells from heterogenous cell populations, allowing downstream single-cell analysis of DNA, RNA and protein. Taken together, the core will directly support the aims of two COBRE projects and enhance the scope and quality of 7 additional research groups within the Biology Department as well as other UNR investigators.

项目总结/摘要 分子成像和媒体核心 分子成像和媒体和显微镜（核心D）成立于第一阶段的第四年， 直接受益于两个COBRE项目研究人员和其他教师和学生使用基本分子 接近UNR。核心将由亚历山大货车德林登博士（毕业于 COBRE项目负责人），并将提供高质量的基础设施，培训和设备 生物分子分析、介质和样品制备资源以及显微镜。核心容纳一个 Biorad V3 Chemidoc和GE Typhoon FLA 7000 IP成像仪，可对DNA进行高分辨率数字成像， RNA、蛋白质和染料，例如化学发光和放射性同位素标记。核还 包含两个Biorad CFX 96实时PCR，用于检测和定量基因转录水平。在 此外，芯部容纳连接到分配器的Integra Mediaclave 10和其他附件， 果蝇和C. elegans研究。该核心在第二阶段的主要目标是 通过提供讲习班、培训和技术培训，进一步扩大基础研究的范围和提高其质量。 向用户提供核心工具的专门知识。第二阶段的核心还将建立一个新的中央设施， 果蝇的饲养和维护，这将直接促进5个研究小组的研究 包括两名COBRE项目负责人第二阶段的核心将扩大，以提供培训和支助， 在生物系共享共聚焦显微镜，这将是共同管理的神经COBRE和 细胞生物学COBRE.此外，作为机构承诺的一部分，第二阶段的核心将扩大 使用激光捕获显微切割镜从异源细胞群中分离特定细胞， 允许DNA、RNA和蛋白质的下游单细胞分析。总的来说，核心将直接 支持两个COBRE项目的目标，并提高另外7个研究小组的范围和质量 以及其他UNR调查人员的调查