Cellular and Molecular Imaging Core

项目主任/首席调查员(最后、第一、中间)：迈克尔·A·韦伯斯特 项目摘要/摘要 细胞和分子成像核心 第三阶段细胞和分子成像(CMI)核心的目标是为Cobre研究人员提供 内华达大学雷诺分校(UNR)面向神经科学研究的最先进的成像工具和培训。 核心提供双光子、共聚焦和广域雷电显微镜的设备和专业知识 在可视化、量化和解释细胞、组织、整体 器官和小动物。核心还提供分子成像设备，用于检测、量化、 以及生物分子样本的分析，如DNA、RNA和蛋白质。核心将协助调查人员 收集图像数据和分析，以进一步研究、促进出版和加强其 争取校外赠款资金的竞争力。核心设备集中在生物系， 与Cobre研究人员关系密切，并成功开发了广泛的用户基础(19个研究实验室 和计数)以及用户之间的高水平交互。《核心》将由范德林登博士执导，他 在第二阶段开发了核心。在第二阶段取得成功的基础上，核心将扩大和加速 Cobre调查人员和其他人的研究生产力具有以下具体目标：1)提供持续的 为研究需要获得高质量显微镜的研究人员提供接触和技术支持 使用我们现有的和最近添加的技术和成像平台获取分子数据。核心意志 还利用由前细胞生物学科布雷中心支持的现代化翻新的组织培养设施； 2)共同提供细胞和分子成像研究和资源方面的途径、培训和教育 需要图像专业知识和实验帮助来收集、分析和 释义。因此，核心将招聘一名新的成像专家，以满足日益增长的显微镜需求 3)建立具有成本效益的服务，以建立可持续的核心设施。总之，CMI核心资源 服务将为Cobre调查人员和其他人创造一条利用先进成像技术的途径， 允许他们将他们的研究带入新的方向和发现，重点是获得 神经系统功能和发育及其相关疾病的基本知识。 OMB编号0925-0001/0002(批准的第03/2020版至2023年2月28日)续页格式页

Program Director/Principal Investigator (Last, First, Middle): Michael A. Webster PROJECT SUMMARY/ABSTRACT Cellular and Molecular Imaging Core The goal of the Phase III Cellular and Molecular Imaging (CMI) Core is to provide COBRE investigators at the University of Nevada, Reno (UNR) state-of-the-art imaging tools and training in neuroscience-oriented research. The Core provides equipment and expertise in two-photon, confocal and wide-field Thunder microscopy with advanced computational capabilities in the visualization, quantification and interpretation of cells, tissues, whole organs, and small animals. The Core also provides molecular imaging equipment for the detection, quantification, and analysis of biomolecular samples such as DNA, RNA, and protein. The Core will assist investigators with collecting image data and analysis to further their research, facilitate publication and to enhance their competitiveness for extramural grant funding. The Core equipment is centrally located in the Biology Department, in close proximity to COBRE investigators, and has successfully developed a wide user base (19 research labs and counting) with high level of interactions among users. The Core will be directed by Dr. van der Linden, who developed the core in Phase II. Building on its success during Phase II, the Core will expand and accelerate research productivity of COBRE investigators and others with the following specific aims: 1) To provide continued access and technical support to investigators whose research requires the acquisition of high-quality microscopy and molecular data using our existing and recently added technologies and imaging platforms. The Core will also leverage a modern renovated Tissue Culture facility supported by the former Cell Biology COBRE center; 2) To provide access, training and education in cellular and molecular imaging research and resources, together with imaging expertise and experimental assistance needed for image data collection, analysis, and interpretation. As such, the Core will recruit a new Imaging Specialist to meet the increasing needs of microscopy imaging; 3) To establish cost-effective services towards a sustainable core facility. In sum, CMI Core resources and services will create a path for COBRE investigators and others to exploit advanced imaging technologies, allowing them to take their research into new directions and discoveries with an emphasis on gaining fundamental knowledge in nervous system function and development, and its associated diseases. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page