Cellular and Molecular Imaging Core

项目负责人/主要研究者（最后，第一，中间）：Michael A.韦伯斯特 项目概要/摘要 细胞和分子成像核心 III期细胞和分子成像（CMI）核心的目标是为COBRE研究人员提供 内华达州大学，里诺（UNR）最先进的成像工具和神经科学研究培训。 核心提供双光子，共聚焦和宽场雷霆显微镜的设备和专业知识， 先进的计算能力，在可视化，量化和解释细胞，组织，整个 器官和小动物。核心还提供分子成像设备，用于检测，定量， 和生物分子样品如DNA、RNA和蛋白质的分析。核心将协助调查人员， 收集图像数据和分析，以促进他们的研究，促进出版，并提高他们的 竞争力的校外赠款资金。核心设备集中在生物系， 与COBRE研究人员非常接近，并成功地开发了广泛的用户群（19个研究实验室 和计数），在用户之间具有高水平的交互。核心将由货车德林登博士指导， 在第二阶段开发了核心。在第二阶段取得成功的基础上， COBRE研究人员和其他人的研究生产力，具体目标如下：1）提供持续的 为研究需要高质量显微镜的研究人员提供访问和技术支持 和分子数据，使用我们现有的和最近增加的技术和成像平台。芯会 还利用由前细胞生物学COBRE中心支持的现代化翻新的组织培养设施; 2)提供细胞和分子成像研究和资源方面的访问、培训和教育， 具有图像数据收集、分析和分析所需的成像专业知识和实验协助， 解释。因此，核心将招募一名新的成像专家，以满足日益增长的显微镜需求 3）建立具有成本效益的服务，以实现可持续的核心设施。总之，CMI核心资源 和服务将为COBRE调查人员和其他人开辟一条利用先进成像技术的道路， 允许他们把他们的研究到新的方向和发现，重点是获得 神经系统功能和发育及其相关疾病的基础知识。 OMB编号0925-0001/0002（修订版03/2020批准至02/28/2023）页码继续格式页码

Program Director/Principal Investigator (Last, First, Middle): Michael A. Webster PROJECT SUMMARY/ABSTRACT Cellular and Molecular Imaging Core The goal of the Phase III Cellular and Molecular Imaging (CMI) Core is to provide COBRE investigators at the University of Nevada, Reno (UNR) state-of-the-art imaging tools and training in neuroscience-oriented research. The Core provides equipment and expertise in two-photon, confocal and wide-field Thunder microscopy with advanced computational capabilities in the visualization, quantification and interpretation of cells, tissues, whole organs, and small animals. The Core also provides molecular imaging equipment for the detection, quantification, and analysis of biomolecular samples such as DNA, RNA, and protein. The Core will assist investigators with collecting image data and analysis to further their research, facilitate publication and to enhance their competitiveness for extramural grant funding. The Core equipment is centrally located in the Biology Department, in close proximity to COBRE investigators, and has successfully developed a wide user base (19 research labs and counting) with high level of interactions among users. The Core will be directed by Dr. van der Linden, who developed the core in Phase II. Building on its success during Phase II, the Core will expand and accelerate research productivity of COBRE investigators and others with the following specific aims: 1) To provide continued access and technical support to investigators whose research requires the acquisition of high-quality microscopy and molecular data using our existing and recently added technologies and imaging platforms. The Core will also leverage a modern renovated Tissue Culture facility supported by the former Cell Biology COBRE center; 2) To provide access, training and education in cellular and molecular imaging research and resources, together with imaging expertise and experimental assistance needed for image data collection, analysis, and interpretation. As such, the Core will recruit a new Imaging Specialist to meet the increasing needs of microscopy imaging; 3) To establish cost-effective services towards a sustainable core facility. In sum, CMI Core resources and services will create a path for COBRE investigators and others to exploit advanced imaging technologies, allowing them to take their research into new directions and discoveries with an emphasis on gaining fundamental knowledge in nervous system function and development, and its associated diseases. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page