Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
批准号:
9404641
负责人:
Emily Gibson
金额:
$70.88万
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-09-30 至 2020-08-31
关键词:
Animal ModelAnimalsAreaBRAIN initiativeBehaviorBrainBrain imagingBrain regionCalciumCaliberCell Culture TechniquesCellsCoupledCustomDataDecision MakingDevicesDiseaseEngineeringFiberGenerationsHeadHealthHippocampus (Brain)ImageIndividualLabelLasersLateralLightLightingLocationMicroscopeMicroscopyMovementMusNervous system structureNeuronsNeurophysiology - biologic functionOperative Surgical ProceduresOpsinOpticsPatternPhenotypePhototoxicityPhysiologic pulseProcessPublicationsResolutionSiteSolidSongbirdsSystemTechnologyTestingThree-Dimensional ImagingTissuesValidationWeightWorkawakebasebrain tissuecalcium indicatordesignflexibilityfluorescence imagingfluorescence microscopeimplantationimprovedin vivoinnovationinstrumentinterestlenslight scatteringlight weightmeetingsmind controlminiaturizemulti-electrode arraysmultidisciplinarymyelinationneural circuitnonhuman primatenovelolfactory bulboptical fiberoptical imagingoptogeneticsphotoactivationrelating to nervous systemresponsesensorsensory inputsocial communicationtwo-photonvocal learning
中文摘要
这是一个响应RFA-NS-17-00 4“BRAIN Initiative: Optimization of Transformative”的应用
英文摘要
This is an application in response to RFA-NS-17-00 4 “BRAIN Initiative: Optimization of Transformative
Technologies for Large Scale Recording and Modulation in the Nervous System (U01)”. In a proof-of-concept
publication we demonstrated a novel fiber-coupled miniature microscope that achieved 3D confocal imaging of
fluorescently labeled neurons in brain tissue using electrowetting lens technology for axial focusing. This low
weight instrument (<2 g) is suitable for surgical implantation to achieve all optical awake behaving recording
with axial focusing deep in the mouse brain, a significant advance compared to commercially available devices.
In this proposal we present a major breakthrough demonstrating successful imaging through the fiber coupled
microscope using two-photon microscopy. Leveraging on this result we propose to optimize our proof of
concept E-FCM (electrowetting-fiber coupled microscope) by making it compatible for two-photon excitation
(2PE-FCM) suitable for recording and modulating neural activity in awake behaving mice. Our 2PE-FCM will
incorporate holographic two-photon opsin stimulation with a spatial light modulator (SLM) for rapid 3D
volumetric two-photon imaging and modulation of neuronal activity. Optimization of the 2PE-FCM will be
achieved by regular interactions of beta users with a multidisciplinary group of physicists, engineers and
neuroscientists in a parallel process of design implementation and testing the device. Testing of the 2PE-FCM
will employ a step-by-step approach designed for effective generation of a device suitable for studying local
circuits in different brain areas in different species. We propose a solid dissemination plan partnering with
Intelligent Imaging Innovations Inc. to commercialize the device. Validation of the 2PE-FCM will be a major
advance in all-optical interrogation of neural circuits creating a new venue for understanding the neural basis
of behavior.
Aim 1. TECHNICAL APPROACH: Optogenetic activation and simultaneous readout of deep
brain neurons using a novel fiber coupled microscope with a variable focus electrowetting lens
using no moving parts.
Aim 2. MILESTONES AND TIMELINE: Validate and disseminate the 2PE-FCM device for
studies of the neural basis of behavior in freely behaving animals.
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会议论文
Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and Modulation
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批准号:10294019
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项目类别:
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财政年份:2021
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负责人:Emily Gibson
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依托单位:
Shedding light on brain circuits mediating navigation of the odor plume in a natural environment
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批准号:10241846
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项目类别:
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资助金额:$8.95万
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财政年份:2020
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负责人:Emily Gibson
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依托单位:
Shedding light on brain circuits mediating navigation of the odor plume in a natural environment
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批准号:10216476
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项目类别:
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资助金额:$27.18万
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财政年份:2020
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负责人:Emily Gibson
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依托单位:
3D-Fast Optical Interface for Rapid Volumetric Neural Sensing and Modulation
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批准号:9764370
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项目类别:
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资助金额:$18.97万
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财政年份:2018
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负责人:Emily Gibson
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依托单位:
Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
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批准号:9770567
-
项目类别:
-
资助金额:$80.09万
-
财政年份:2017
-
负责人:Emily Gibson
-
依托单位:
海外基金