Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
使用两个光子空间形状光遗传学控制体内神经元放电
基本信息
- 批准号:9404641
- 负责人:
- 金额:$ 70.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-30 至 2020-08-31
- 项目状态:已结题
- 来源:
- 关键词:Animal ModelAnimalsAreaBRAIN initiativeBehaviorBrainBrain imagingBrain regionCalciumCaliberCell Culture TechniquesCellsCoupledCustomDataDecision MakingDevicesDiseaseEngineeringFiberGenerationsHeadHealthHippocampus (Brain)ImageIndividualLabelLasersLateralLightLightingLocationMicroscopeMicroscopyMovementMusNervous system structureNeuronsNeurophysiology - biologic functionOperative Surgical ProceduresOpsinOpticsPatternPhenotypePhototoxicityPhysiologic pulseProcessPublicationsResolutionSiteSolidSongbirdsSystemTechnologyTestingThree-Dimensional ImagingTissuesValidationWeightWorkawakebasebrain tissuecalcium indicatordesignflexibilityfluorescence imagingfluorescence microscopeimplantationimprovedin vivoinnovationinstrumentinterestlenslight scatteringlight weightmeetingsmind controlminiaturizemulti-electrode arraysmultidisciplinarymyelinationneural circuitnonhuman primatenovelolfactory bulboptical fiberoptical imagingoptogeneticsphotoactivationrelating to nervous systemresponsesensorsensory inputsocial communicationtwo-photonvocal learning
项目摘要
This is an application in response to RFA-NS-17-00 4 “BRAIN Initiative: Optimization of Transformative
Technologies for Large Scale Recording and Modulation in the Nervous System (U01)”. In a proof-of-concept
publication we demonstrated a novel fiber-coupled miniature microscope that achieved 3D confocal imaging of
fluorescently labeled neurons in brain tissue using electrowetting lens technology for axial focusing. This low
weight instrument (<2 g) is suitable for surgical implantation to achieve all optical awake behaving recording
with axial focusing deep in the mouse brain, a significant advance compared to commercially available devices.
In this proposal we present a major breakthrough demonstrating successful imaging through the fiber coupled
microscope using two-photon microscopy. Leveraging on this result we propose to optimize our proof of
concept E-FCM (electrowetting-fiber coupled microscope) by making it compatible for two-photon excitation
(2PE-FCM) suitable for recording and modulating neural activity in awake behaving mice. Our 2PE-FCM will
incorporate holographic two-photon opsin stimulation with a spatial light modulator (SLM) for rapid 3D
volumetric two-photon imaging and modulation of neuronal activity. Optimization of the 2PE-FCM will be
achieved by regular interactions of beta users with a multidisciplinary group of physicists, engineers and
neuroscientists in a parallel process of design implementation and testing the device. Testing of the 2PE-FCM
will employ a step-by-step approach designed for effective generation of a device suitable for studying local
circuits in different brain areas in different species. We propose a solid dissemination plan partnering with
Intelligent Imaging Innovations Inc. to commercialize the device. Validation of the 2PE-FCM will be a major
advance in all-optical interrogation of neural circuits creating a new venue for understanding the neural basis
of behavior.
Aim 1. TECHNICAL APPROACH: Optogenetic activation and simultaneous readout of deep
brain neurons using a novel fiber coupled microscope with a variable focus electrowetting lens
using no moving parts.
Aim 2. MILESTONES AND TIMELINE: Validate and disseminate the 2PE-FCM device for
studies of the neural basis of behavior in freely behaving animals.
这是一个响应RFA-NS-17-00 4“Brain Initiative:优化变革性的
神经系统的大规模记录和调制技术(U01)“。在概念验证中
我们展示了一种新型的光纤耦合微型显微镜,它实现了三维共聚焦成像
使用电润湿透镜技术进行轴向聚焦的脑组织荧光标记神经元。这么低
重量仪(<;2g)适用于外科植入,实现全光清醒行为记录
由于轴向聚焦深入小鼠大脑,与商用设备相比,这是一个重大进步。
在这份提案中,我们展示了一项重大突破,展示了通过光纤耦合的成功成像
显微镜使用双光子显微镜。利用这一结果,我们建议优化我们的证明
使其兼容双光子激发的概念E-FCM
(2pE-FCM),适用于记录和调节清醒行为小鼠的神经活动。我们的2PE-FCM将
将全息双光子光刺激与空间光调制器(SLM)相结合实现快速3D
体积双光子成像和神经元活动的调节。2PE-FCM的优化将是
通过测试版用户与多学科的物理学家、工程师和
神经学家正在同时设计、实施和测试该设备。2PE-FCM的检测
将采用一种循序渐进的方法,旨在有效地产生适合于研究当地的设备
不同物种不同脑区的回路。我们提出了一个坚实的传播计划,与
智能成像创新公司将该设备商业化。2PE-FCM的验证将是一个主要的
神经回路全光询问的进展为理解神经基础创造了一个新的场所
关于行为的。
目的1.技术途径:光遗传激活和同时读出DEEP
新型变焦电润湿镜光纤耦合显微镜在脑神经元中的应用
不使用活动部件。
目标2.里程碑和时间表:验证和传播2PE-FCM设备
对自由行为动物行为的神经基础的研究。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Emily Gibson其他文献
Emily Gibson的其他文献
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{{ truncateString('Emily Gibson', 18)}}的其他基金
Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and Modulation
开发用于快速体积神经传感和调制的 3D-FAST 光学接口
- 批准号:
10294019 - 财政年份:2021
- 资助金额:
$ 70.88万 - 项目类别:
Shedding light on brain circuits mediating navigation of the odor plume in a natural environment
揭示自然环境中调节气味羽流的大脑回路
- 批准号:
10241846 - 财政年份:2020
- 资助金额:
$ 70.88万 - 项目类别:
Shedding light on brain circuits mediating navigation of the odor plume in a natural environment
揭示自然环境中调节气味羽流的大脑回路
- 批准号:
10216476 - 财政年份:2020
- 资助金额:
$ 70.88万 - 项目类别:
3D-Fast Optical Interface for Rapid Volumetric Neural Sensing and Modulation
用于快速体积神经传感和调制的 3D 快速光学接口
- 批准号:
9764370 - 财政年份:2018
- 资助金额:
$ 70.88万 - 项目类别:
Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
使用两个光子空间形状光遗传学控制体内神经元放电
- 批准号:
9770567 - 财政年份:2017
- 资助金额:
$ 70.88万 - 项目类别:
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