Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
使用两个光子空间形状光遗传学控制体内神经元放电
基本信息
- 批准号:9404641
- 负责人:
- 金额:$ 70.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-30 至 2020-08-31
- 项目状态:已结题
- 来源:
- 关键词:Animal ModelAnimalsAreaBRAIN initiativeBehaviorBrainBrain imagingBrain regionCalciumCaliberCell Culture TechniquesCellsCoupledCustomDataDecision MakingDevicesDiseaseEngineeringFiberGenerationsHeadHealthHippocampus (Brain)ImageIndividualLabelLasersLateralLightLightingLocationMicroscopeMicroscopyMovementMusNervous system structureNeuronsNeurophysiology - biologic functionOperative Surgical ProceduresOpsinOpticsPatternPhenotypePhototoxicityPhysiologic pulseProcessPublicationsResolutionSiteSolidSongbirdsSystemTechnologyTestingThree-Dimensional ImagingTissuesValidationWeightWorkawakebasebrain tissuecalcium indicatordesignflexibilityfluorescence imagingfluorescence microscopeimplantationimprovedin vivoinnovationinstrumentinterestlenslight scatteringlight weightmeetingsmind controlminiaturizemulti-electrode arraysmultidisciplinarymyelinationneural circuitnonhuman primatenovelolfactory bulboptical fiberoptical imagingoptogeneticsphotoactivationrelating to nervous systemresponsesensorsensory inputsocial communicationtwo-photonvocal learning
项目摘要
This is an application in response to RFA-NS-17-00 4 “BRAIN Initiative: Optimization of Transformative
Technologies for Large Scale Recording and Modulation in the Nervous System (U01)”. In a proof-of-concept
publication we demonstrated a novel fiber-coupled miniature microscope that achieved 3D confocal imaging of
fluorescently labeled neurons in brain tissue using electrowetting lens technology for axial focusing. This low
weight instrument (<2 g) is suitable for surgical implantation to achieve all optical awake behaving recording
with axial focusing deep in the mouse brain, a significant advance compared to commercially available devices.
In this proposal we present a major breakthrough demonstrating successful imaging through the fiber coupled
microscope using two-photon microscopy. Leveraging on this result we propose to optimize our proof of
concept E-FCM (electrowetting-fiber coupled microscope) by making it compatible for two-photon excitation
(2PE-FCM) suitable for recording and modulating neural activity in awake behaving mice. Our 2PE-FCM will
incorporate holographic two-photon opsin stimulation with a spatial light modulator (SLM) for rapid 3D
volumetric two-photon imaging and modulation of neuronal activity. Optimization of the 2PE-FCM will be
achieved by regular interactions of beta users with a multidisciplinary group of physicists, engineers and
neuroscientists in a parallel process of design implementation and testing the device. Testing of the 2PE-FCM
will employ a step-by-step approach designed for effective generation of a device suitable for studying local
circuits in different brain areas in different species. We propose a solid dissemination plan partnering with
Intelligent Imaging Innovations Inc. to commercialize the device. Validation of the 2PE-FCM will be a major
advance in all-optical interrogation of neural circuits creating a new venue for understanding the neural basis
of behavior.
Aim 1. TECHNICAL APPROACH: Optogenetic activation and simultaneous readout of deep
brain neurons using a novel fiber coupled microscope with a variable focus electrowetting lens
using no moving parts.
Aim 2. MILESTONES AND TIMELINE: Validate and disseminate the 2PE-FCM device for
studies of the neural basis of behavior in freely behaving animals.
这是响应RFA-NS-17-00 4“BRAIN倡议:
神经系统大规模记录和调制技术(U 01)。在概念验证中,
我们展示了一种新型的光纤耦合微型显微镜,实现了3D共焦成像,
使用用于轴向聚焦的电润湿透镜技术在脑组织中荧光标记的神经元。这种低
重量仪器(<2g)适合于外科植入以实现全光学清醒行为记录
轴向聚焦在小鼠大脑深处,与商业上可用的设备相比,这是一个重大的进步。
在这项提案中,我们提出了一个重大突破,证明通过光纤耦合成功成像
双光子显微镜(Two Photon Microscopy)利用这一结果,我们建议优化我们的证明,
概念E-FCM(电润湿-光纤耦合显微镜),使其兼容双光子激发
(2 PE-FCM)适合于记录和调节清醒行为小鼠中的神经活动。我们的2 PE-FCM将
结合全息双光子视蛋白刺激与空间光调制器(SLM)用于快速3D
体积双光子成像和神经元活动的调制。2 PE-FCM的优化将是
通过beta用户与物理学家,工程师和
神经科学家在设计实施和测试设备的并行过程中。2 PE-FCM的测试
将采用一步一步的方法,旨在有效地产生一个适合研究当地的设备,
不同物种大脑不同区域的神经回路。我们提出了一个坚实的传播计划,
智能成像创新公司将该设备商业化。2 PE-FCM的验证将是
神经回路的全光学询问的进展为理解神经基础创造了新的场所
的行为。
目标1。技术方法:光遗传激活和同时读出深度
使用具有可变焦点电润湿透镜的新型纤维耦合显微镜的脑神经元
不使用移动部件。
目标二。里程碑和时间轴:宣传和推广2 PE-FCM设备,
对行为自由的动物行为的神经基础的研究。
项目成果
期刊论文数量(0)
专著数量(0)
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专利数量(0)
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Emily Gibson其他文献
Emily Gibson的其他文献
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{{ truncateString('Emily Gibson', 18)}}的其他基金
Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and Modulation
开发用于快速体积神经传感和调制的 3D-FAST 光学接口
- 批准号:
10294019 - 财政年份:2021
- 资助金额:
$ 70.88万 - 项目类别:
Shedding light on brain circuits mediating navigation of the odor plume in a natural environment
揭示自然环境中调节气味羽流的大脑回路
- 批准号:
10241846 - 财政年份:2020
- 资助金额:
$ 70.88万 - 项目类别:
Shedding light on brain circuits mediating navigation of the odor plume in a natural environment
揭示自然环境中调节气味羽流的大脑回路
- 批准号:
10216476 - 财政年份:2020
- 资助金额:
$ 70.88万 - 项目类别:
3D-Fast Optical Interface for Rapid Volumetric Neural Sensing and Modulation
用于快速体积神经传感和调制的 3D 快速光学接口
- 批准号:
9764370 - 财政年份:2018
- 资助金额:
$ 70.88万 - 项目类别:
Controlled neuronal firing in vivo using two photon spatially shaped optogenetics
使用两个光子空间形状光遗传学控制体内神经元放电
- 批准号:
9770567 - 财政年份:2017
- 资助金额:
$ 70.88万 - 项目类别:
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