Senescence tissue mapping and SASP Atlas for human somatic and reproductive tissues

行政核心--项目摘要 巴克研究所组织测绘中心(TMC)的这项拨款申请建议将衰老细胞映射成三个 人的躯体和生殖组织，即卵巢、乳房组织和骨骼肌。关键是要提供 细胞衰老的组织图以及这些人体组织的SASP图谱，因为(I)卵巢是 最早老化的组织，(Ii)乳腺组织已显示出快速老化并显示高SASP基因表达 以前的RNA序列实验中的签名，以及(Iii)肌肉老化通常会导致行动不便或虚弱。 行政核心将监督领导计划、组织图协调计划和网络协调 计划生成衰老组织图，作为Sennet联盟的一部分。管理核心将对此进行监督 将实施和改进TMC内部和与Sennet网络的数据共享管道。这个 管理核心与Buck Institute TMC的其他3个科学核心Biosecimen之间的合作 核心，生物分析核心和数据分析核心，将实现简化的组织收集，数据 获取和数据分析流水线，以生成和优先处理以 体细胞和生殖组织。行政核心领导和行政核心领导之间的定期讨论和会议 其他科学核心的领导人将允许所有利益攸关方之间进行有效的信息交流。这个 管理核心将与数据分析核心协作，制定和实施提交 向Sennet联盟及其数据协调中心提供数据、协议和资源。管理核心将 确保提交给财团的任何数据都符合全网标准操作程序 和Sennet保单。与其他TMC中心的合作将使衰老标志物的比较成为可能 在组织之间，并应用在不同组织TMC中心可用的不同技术 绘制多个组织图，因为管理中心将鼓励所有TMC中心合作并参与 在技术技能的交叉培训中，在中心之间为科学家使用新的仪器，以及 扩展关于细胞衰老的知识。在整个获奖期内，新颖的技术 改进将更广泛地实施到TMC工作流程和组织生物分析管道中，作为 它们可用于网络使用。管理核心将在协作中协调这些实施 与其他技术开发中心和其他TMC中心合作。 总而言之，行政核心将协调TMC的所有努力，并在现有强大的科学 巴克研究所在细胞衰老研究方面的专长，以建立新的衰老组织图谱 图集资源侧重于人类卵巢、乳房组织和骨骼肌。

ADMINISTRATIVE CORE - PROJECT SUMMARY This Buck Institute Tissue Mapping Center (TMC) grant application proposes to map senescent cells in three human somatic and reproductive tissues, i.e., ovaries, breast tissue, and skeletal muscle. It is crucial to provide tissue maps of cellular senescence as well as a SASP Atlas of for these human tissues as (i) ovaries are one of the first tissues to age, (ii) breast tissue has been shown to rapidly age and display high SASP gene expression signatures in previous RNA Seq experiments, and (iii) muscle aging typically leads to impacted mobility or frailty. The Admin core will oversee a leadership plan, a tissue-mapping coordination plan and a network coordination plan to generate senescence tissue maps as part of the SenNet Consortium. The Admin Core will oversee that data sharing pipelines within the TMC and with the SenNet network will be implemented and improved. The collaboration between the Admin Core with the 3 other scientific Buck Institute TMC cores, the Biospecimen Core, Biological Analysis Core and Data Analysis Core, will enable a streamlined tissue collection, data acquisition and data analysis pipeline, in order to generate and prioritize senescence biomarkers expressed in the somatic and reproductive tissues. Regular discussions and meetings between the Admin Core leaders and leaders of the other scientific cores will allow efficient information exchange between all stakeholders. The Admin Core in collaboration with the Data Analysis Core will develop and implement plans for submission of data, protocols and resources to the SenNet Consortium and its data coordination center. The Admin Core will ensure that any data submitted to the consortium complies with network-wide standard operating procedures and SenNet policies. Collaborations with other TMC centers will enable comparisons of senescence markers between tissues, and applying different technologies that are available at different TMC centers for tissue mapping of multiple tissues, as the Admin Center will encourage all TMC centers to work together and to engage in cross-training of technological skill-sets, use of novel instrumentations for scientists between centers, and expanding intellectual knowledge about cellular senescence. Throughout the award period novel technological advancements will be implemented more broadly into TMC workflows and tissue biological analysis pipelines as they become available for network use. The Admin Core will coordinate such implementation in collaboration with other Technology Development Centers and other TMC centers. In summary, the Admin Core will coordinate all efforts of the TMC and build upon the existing strong scientific expertise in cellular senescence research at the Buck Institute to establish a new Senescence Tissue Mapping Atlas resource focusing on human ovaries, breast tissue, and skeletal muscle.