Senescence tissue mapping and SASP Atlas for human somatic and reproductive tissues

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ADMINISTRATIVE CORE - PROJECT SUMMARY This Buck Institute Tissue Mapping Center (TMC) grant application proposes to map senescent cells in three human somatic and reproductive tissues, i.e., ovaries, breast tissue, and skeletal muscle. It is crucial to provide tissue maps of cellular senescence as well as a SASP Atlas of for these human tissues as (i) ovaries are one of the first tissues to age, (ii) breast tissue has been shown to rapidly age and display high SASP gene expression signatures in previous RNA Seq experiments, and (iii) muscle aging typically leads to impacted mobility or frailty. The Admin core will oversee a leadership plan, a tissue-mapping coordination plan and a network coordination plan to generate senescence tissue maps as part of the SenNet Consortium. The Admin Core will oversee that data sharing pipelines within the TMC and with the SenNet network will be implemented and improved. The collaboration between the Admin Core with the 3 other scientific Buck Institute TMC cores, the Biospecimen Core, Biological Analysis Core and Data Analysis Core, will enable a streamlined tissue collection, data acquisition and data analysis pipeline, in order to generate and prioritize senescence biomarkers expressed in the somatic and reproductive tissues. Regular discussions and meetings between the Admin Core leaders and leaders of the other scientific cores will allow efficient information exchange between all stakeholders. The Admin Core in collaboration with the Data Analysis Core will develop and implement plans for submission of data, protocols and resources to the SenNet Consortium and its data coordination center. The Admin Core will ensure that any data submitted to the consortium complies with network-wide standard operating procedures and SenNet policies. Collaborations with other TMC centers will enable comparisons of senescence markers between tissues, and applying different technologies that are available at different TMC centers for tissue mapping of multiple tissues, as the Admin Center will encourage all TMC centers to work together and to engage in cross-training of technological skill-sets, use of novel instrumentations for scientists between centers, and expanding intellectual knowledge about cellular senescence. Throughout the award period novel technological advancements will be implemented more broadly into TMC workflows and tissue biological analysis pipelines as they become available for network use. The Admin Core will coordinate such implementation in collaboration with other Technology Development Centers and other TMC centers. In summary, the Admin Core will coordinate all efforts of the TMC and build upon the existing strong scientific expertise in cellular senescence research at the Buck Institute to establish a new Senescence Tissue Mapping Atlas resource focusing on human ovaries, breast tissue, and skeletal muscle.