Application of Flow Cytometry to Cell Biology

实验免疫学分部(EIB)流式细胞仪实验室目前支持50多名研究人员的多个研究项目。这些研究包括对新鲜从不同物种和/或组织制备的细胞以及体外培养细胞的光谱进行多参数定量单细胞分析和基于与细胞相关的参数的电子细胞分离。基础研究支持提供给EIB成员和NCI癌症研究中心内的其他研究人员。目前得到支持的项目包括但不限于以下研究领域：a)通过细胞表面分子进行的细胞内信号传递的体内和体外分析；b)动物和/或基因修饰细胞的细胞过程和/或缺陷的分析；c)免疫发病机制和后果的研究；d)各种分子的细胞表面配位表达的分析；e)T细胞库的研究；g)移植抗原的表达分析；h)T细胞谱系发育所涉及的机制的研究；以及i)细胞死亡的机制。

The Experimental Immunology Branch (EIB) flow cytometry laboratory currently supports multiple research projects for more than 50 investigators. These investigations involve multiparametric quantitative single cell analysis of, and electronic cell separation based upon, parameters associated with cells freshly prepared from different species and/or tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and to other investigators within the Center for Cancer Research, NCI. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signaling via cell surface molecules; b) analyses of cellular processes and/or defects in animals and/or cells with genetic modifications; c) studies of the mechanisms and consequences of immune pathogenesis; d) analyses of the coordinate cell surface expression of a variety of molecules; e) investigations of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in T cell lineage development; and i) mechanisms of cell death.