Application of Flow Cytometry to Cell Biology

流式细胞术在细胞生物学中的应用

• 批准号: 7048820
• 负责人: SUSAN SHARROW
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7048820
• 关键词: T lymphocyte; biomedical resource; cell biology; cell sorting; flow cytometry; human tissue; single cell analysis; tissue /cell culture; tissue /cell preparation

The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 50 investigators. These investigations involve multiparametric quantitative single cell analysis of, and electronic cell separation based upon, parameters associated with cells freshly prepared from different species and/or tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and to other investigators within DBS. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signaling via T cell surface molecules; b) analyses of cellular processes and/or defects in animals and/or cells with genetic modifications; c) studies of the pathogenesis of graft-versus host disease; d) analyses of the coordinate cell surface expression of a variety of molecules; e) investigations of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in T cell lineage development; and i) mechanisms of cell death.

实验免疫学分支流式细胞术实验室目前支持50多名研究人员的多个研究项目。这些调查涉及多参数定量单细胞分析，和电子细胞分离的基础上，从不同物种和/或组织新鲜制备的细胞，以及在体外培养的细胞谱相关的参数。基础研究支持提供给EIB的成员和DBS内的其他研究人员。目前资助的项目包括但不限于以下研究领域：a）通过T细胞表面分子进行细胞内信号传导的体内和体外分析; B）分析动物和/或经遗传修饰的细胞的细胞过程和/或缺陷; c）研究移植物抗宿主病的发病机理; d）分析各种分子的协调细胞表面表达; e）研究T细胞库的产生; g）分析移植抗原的表达; h）研究涉及T细胞谱系发育的机制;和i）细胞死亡的机制。