TOPIC 382 - INTEGRATED IMAGING AND MOLECULAR ANALYSIS OF GLIOBLASTOMA USING SCOPE SEQ ON THE AIR FLOW

主题 382 - 使用 SCOPE SEQ 在气流上对胶质母细胞瘤进行综合成像和分子分析

• 批准号: 10030959
• 负责人: STEVEN GEBHART
• 金额: $ 29.76万
• 依托单位: CELL MICROSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-16 至 2020-06-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10030959
• 关键词: Agreement; Air; Apoptosis; Bioinformatics; Biological Models; Biopsy; Caliber; Capital; Cell Cycle; Cell Cycle Progression; Cell Line; Cell Lineage; Cells; Clinical Trials; Clone Cells; Complement; Computer software; Custom; Cycloparaffins; Data; Data Quality; Data Set; Development; Device or Instrument Development; Disease; Doctor of Philosophy; Dyes; Elastomers; Ensure; Exhibits; Exposure to; Fluorescent Dyes; Functional Imaging; Genes; Genomics; Glioblastoma; Glioma; Goals; Image; Image Analysis; In Vitro; Injections; Laboratories; Libraries; Link; Malignant Neoplasms; Maps; Memorial Sloan-Kettering Cancer Center; Mesenchymal; Methods; Microfabrication; Microfluidics; Mitochondria; Modality; Molds; Molecular; Molecular Analysis; Operative Surgical Procedures; Optics; Outcome; Periodicity; Pharmaceutical Preparations; Pharmacotherapy; Phase; Phenotype; Physiology; Ploidies; Polymers; Polymethyl Methacrylate; Positioning Attribute; Preparation; Procedures; Process; Property; Protocols documentation; Publications; Publishing; Sampling; Savings; Scanning; Specificity; Stains; Structure; Suspensions; System; Technology; Testing; Universities; Valproic Acid; Work; base; brain tumor resection; cDNA Library; cell type; copolymer; cost; imaging study; improved; in vivo; instrument; lithography; microsystems; neoplastic cell; novel; optical spectra; polydimethylsiloxane; programs; prototype; research clinical testing; response; single cell analysis; software development; success; transcriptomics

Combining both genomic analysis and highEresolution imaging on a cellEbyEcell basis typically relies on customized technology which often requires significant expertise and capital cost. Cell Microsystems, Inc. has partnered with Peter Sims, PhD of Columbia University to commercialize a novel workflow for integrated imaging and sample preparation for single cell transcriptomic analysis. Dr. Sims workflow, Single Cell Optical Phenotyping and Expression Sequencing (SCOPEESeq), will be automated on a new microfluidic instrument under development by Cell Microsystems, the AIREFLOW. The system provides detailed image analysis of single cells, in addition to fullyEautomated sample preparation of single cell transcriptomic analysis (i.e. scERNAESeq). Here, we will apply this collaboratively developed platform to investigating the intractable cancer, glioblastoma. The Phase I program will use glioblastoma neurospheres as a model system to validate the system’s capabilities for: functional dye imaging, single cell molecular analysis and linking these data modalities on a cellEbyEcell basis. During the proposed Phase II these capabilities will be further tested in patientEderived biopsies to test the tumor cell specificity of fluorescent dyes used during surgical brain tumor resection. We expect the resulting system to have significant impact in this field, as well as considerable commercial potential in the broader market.

在 cellEbyEcell 基础上结合基因组分析和高分辨率成像通常依赖于定制技术，而这通常需要大量的专业知识和资本成本。 Cell Microsystems, Inc. 与哥伦比亚大学博士 Peter Sims 合作，将用于单细胞转录组分析的集成成像和样品制备的新颖工作流程商业化。 Dr. Sims 工作流程、单细胞光学表型分析和表达测序 (SCOPEESeq) 将在 Cell Microsystems 正在开发的新型微流体仪器 AIREFLOW 上实现自动化。 除了单细胞转录组分析（即 scERNAESeq）的全自动样品制备之外，该系统还提供单细胞的详细图像分析。在这里，我们将应用这个合作开发的平台来研究棘手的癌症——胶质母细胞瘤。第一阶段计划将使用胶质母细胞瘤神经球作为模型系统，以验证该系统的功能：功能染料成像、单细胞分子分析以及在 cellEbyEcell 基础上链接这些数据模式。 在拟议的第二阶段期间，这些功能将在患者活检中进一步测试，以测试手术脑肿瘤切除过程中使用的荧光染料的肿瘤细胞特异性。我们预计由此产生的系统将对该领域产生重大影响，并在更广泛的市场中具有巨大的商业潜力。