Cross-sectional associations of oral microbiota with oral HPV infection among high-risk Hispanic adults

高危西班牙裔成年人口腔微生物群与口腔 HPV 感染的横断面关联

• 批准号: 10003477
• 负责人: FILIPA GODOY-VITORINO
• 金额: $ 6.18万
• 依托单位: COMPREHENSIVE CANCER CENTER/ UNIV/PR
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10003477
• 关键词: 16S ribosomal RNA sequencing; Adult; Affect; Anatomy; Bacteria; Bacteroidetes; Behavioral; Biological; Cancer Control; Cancer Diagnostics; Cells; Cervical; Characteristics; Clinic; Clinical; Communities; Computer Assisted; Coupled; Cross-Sectional Studies; Data; Data Analyses; Dental; Dental Epidemiology; Development; Disease; Dot Immunoblotting; Ecology; Ecosystem; Environment; Ethnic group; Firmicutes; Genes; Genomic DNA; Genotype; Gingiva; Goals; HIV; Habitats; High Prevalence; Hispanics; Human; Human Biology; Human Microbiome; Human Papillomavirus; Human papilloma virus infection; Human papillomavirus 16; Immune response; Individual; Infection; Inflammation; Inflammatory; Injections; Interview; Interviewer; Lactobacillus; Lesion; Longitudinal Studies; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Mediating; Medical; Methodology; Microbe; Mouth Neoplasms; Multiple Anatomic Sites; National Health and Nutrition Examination Survey; Natural History; Neisseria; Oncogenic; Oral; Oral Characters; Oral cavity; Oral health; Oropharyngeal; Participant; Patients; Periodontal Diseases; Periodontitis; Pharmaceutical Preparations; Play; Population; Predisposition; Premalignant; Prevalence; Prevention strategy; Preventive; Process; Public Health; Puerto Rican; Puerto Rico; Questionnaires; Recombinant DNA; Research; Resolution; Risk; Risk Behaviors; Risk Factors; Role; Saliva; Sampling; Sexually Transmitted Diseases; Structure of gingival sulcus; Testing; Time; Tongue; Ursidae Family; Virus Diseases; base; cancer health disparity; case control; community living; high risk; high risk population; high throughput technology; malignant mouth neoplasm; malignant oropharynx neoplasm; men; microbial; microbiome; microbiota; microbiota profiles; microorganism; mouth squamous cell carcinoma; next generation sequencing; non-oncogenic; operational taxonomic units; oral cancer prevention; oral carcinogenesis; oral lesion; oral microbial community; oral microbiome; periodontopathogen; profiles in patients; recruit; reproductive tract; social; sociodemographics; socioeconomic disadvantage; tool; vaginal microbiota; viral detection

PROJECT SUMMARY The rising rates of Human Papilloma Virus (HPV)-positive oropharyngeal cancers in the U.S. is a significant public health issue, while Hispanics show increased burden of this malignancy. Recent research suggests differences in oral microbiota between oropharyngeal cancer cases and controls, and between HPV+ and HPV- individuals. Nonetheless, research evaluating the role of oral microbiota on oral HPV infection, a main risk factor for oropharyngeal cancer, is scarce, and essential to develop effective prevention strategies. The proposed cross-sectional study will recruit 300 participants from sexually transmitted infections (STI) clinics of Puerto Rico. The specific aims of this application are to (1) characterize the oral microbiota profiles of patients with and without oral HPV infection, and (2) assess the association between species-level oral microbiota and oral HPV infection. Participants will provide saliva samples for oral HPV detection, following the NHANES methodology, and for oral microbiota analysis. Socio-demographic, behavioral, dental, and medical characteristics will be collected through an interviewer-administered questionnaire. Risk factors for oral HPV infection will be collected using the audio computer-assisted self-interview (ACASI) methodology, a useful tool for collecting sensitive risk behavior data. HPV typing will be performed by dot-blot hybridization using 38 type-specific probes, including oncogenic and non-oncogenic types, while for microbiota, genomic DNA extractions and 16S rDNA V4 amplifications will be done. Samples will then be subjected to Illumina Miseq sequencing and data analyses. To our knowledge, this is the first study that will assess the association between the oral microbiota and oral HPV infection among a homogenous Hispanic population. Accumulating evidence supports a biological plausibility for the association between the microbiota and HPV infection in various anatomical sites and with oral cancer; however, data on its relationship with oral HPV infection is limited. The identification of the oral microbiota’s role on oral HPV infection may provide essential information for the development of preventive public health strategies, as if research shows that the oral microbiota modulates oral HPV infection and oral carcinogenesis, the implications for oral cancer prevention and control strategies might be important.

项目概要 在美国，人乳头瘤病毒 (HPV) 阳性口咽癌发病率的上升是一个重大问题 公共卫生问题，而西班牙裔人则表现出这种恶性肿瘤的负担增加。最近的研究表明 口咽癌病例和对照之间以及 HPV+ 和 HPV- 之间口腔微生物群的差异 个人。尽管如此，有研究评估口腔微生物群对口腔 HPV 感染（主要危险因素）的作用 对于口咽癌来说，这种药物是稀缺的，并且对于制定有效的预防策略至关重要。拟议的 横断面研究将从波多黎各性传播感染 (STI) 诊所招募 300 名参与者。 本申请的具体目的是 (1) 描述患有和不患有这种疾病的患者的口腔微生物群特征。 口腔 HPV 感染，(2) 评估物种水平口腔微生物群与口腔 HPV 感染之间的关联。 参与者将按照 NHANES 方法提供唾液样本用于口腔 HPV 检测，并用于口腔 HPV 检测。 微生物群分析。社会人口、行为、牙科和医疗特征将通过以下方式收集： 由访谈员管理的调查问卷。将使用音频收集口腔 HPV 感染的危险因素 计算机辅助自我访谈（ACASI）方法，是收集敏感风险行为数据的有用工具。 HPV 分型将使用 38 种类型特异性探针通过斑点杂交进行，包括致癌探针和 非致癌类型，而对于微生物群，基因组 DNA 提取和 16S rDNA V4 扩增将 完毕。然后样品将接受 Illumina Miseq 测序和数据分析。据我们所知，这 是第一项评估口腔微生物群与口腔 HPV 感染之间关系的研究 同质的西班牙裔人口。越来越多的证据支持该关联的生物学合理性 不同解剖部位的微生物群与 HPV 感染以及口腔癌之间的关系；然而，其数据 与口腔 HPV 感染的关系有限。口腔微生物群在口腔 HPV 感染中的作用的鉴定 可以为预防性公共卫生战略的制定提供重要信息，就像研究一样 表明口腔微生物群调节口腔 HPV 感染和口腔癌的发生，这对口腔癌的影响 癌症预防和控制策略可能很重要。