Core B - Mutant Mouse

核心 B - 突变小鼠

• 批准号: 10023665
• 负责人: Dario AA Vignali
• 金额: $ 45.85万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10023665
• 关键词: Adoptive Transfer; Age; Autoimmune Diseases; Autoimmunity; Biological; Breeding; Budgets; CD8-Positive T-Lymphocytes; CD8B1 gene; Chronic; Collection; Communities; Complex; Data; Disease; Dissection; Embryo; Ensure; Enterobacteria phage P1 Cre recombinase; FOXP3 gene; Female; Genes; Genetic; Genotype; Health Status; Immunity; Immunologics; Inbred Strains Mice; Individual; Lead; LoxP-flanked allele; Malignant Neoplasms; Mediating; Monitor; Mus; Mutant Strains Mice; Production; Quarantine; Rag1 Mouse; Regulation; Regulatory T-Lymphocyte; Research; Research Personnel; Resources; Role; Scheme; Shipping; Ships; Site; Stains; System; T cell therapy; T-Lymphocyte; Time; Treatment Efficacy; Tumor Immunity; Universities; Variant; Virus Diseases; Work; antiviral immunity; cell type; chronic infection; cost; cost effective; design; exhaust; experimental study; flexibility; genetic element; immunoregulation; in vivo; insight; male; microbiota; mouse development; mouse model; pathogen; performance site; prevent; programmed cell death protein 1; programs; receptor; repository; sperm cell; synergism; therapeutic target; tool; tumor

CORE SUMMARY The Mutant Mouse Core (Core B) is an essential core that will be used extensively by all three projects. Given the complex interplay between different inhibitory receptors on multiple cell types, the only way one can dissect these critical disease-related biological questions in vivo is with sophisticated mouse models. A significant strength of this PPG application is the unique `immunological toolbox' that it will utilize, specifically PD1/LAG3 floxed mice combined with a unique collection of lineage-specific, and in most instances temporally controlled, Cre recombinase-expressing mice. These tools will give us unprecedented control and thus insight into PD1/LAG3 synergy and function in multiple cell types. AIM 1: To provide required PD1/LAG3 mutant mice. The Core will be administered from the University of Pittsburgh (Pitt), with the majority of the mutant mouse breeding and procurement outsourced to Taconic under Murine Pathogen Free barrier conditions identical to the majority of their commercial breeding practices. Taconic will deliver mice to all research sites on a rotating bi-weekly basis using their conventional delivery mechanism, thereby avoiding lengthy quarantine. AIM 2: To generate new mutant mouse combinations. The Core will generate new mutant mouse combinations that introduce different genetic elements such as new Cre stains. PPG Interactions: Core B will support Project 1, Project 2 and Project 3 extensively, and interact with Core A to provide easy access to breeding, shipping and genotyping data. Core B will also perform additional crosses to generate any strain combinations required by the projects. Core B will interact with Core A to provide easy access to breeding, shipping and genotyping data and ongoing monitoring.

核心摘要 突变小鼠核心（核心B）是一个必不可少的核心，将被所有三个项目广泛使用。给定 多种细胞类型上不同抑制性受体之间的复杂相互作用，这是人们可以分析 这些关键的疾病相关的生物学问题在体内是与复杂的小鼠模型。显著 这种PPG应用的优势在于它将使用的独特的“免疫工具箱”，特别是PD1/LAG3 floxed小鼠与独特的谱系特异性的集合相结合，并且在大多数情况下是暂时控制的， Cre重组酶表达小鼠。这些工具将给我们前所未有的控制，从而洞察 PD1/LAG3在多种细胞类型中的协同作用和功能。 目的1：提供所需的PD1/LAG3突变小鼠。核心将由大学管理， 匹兹堡（Pitt），大部分突变小鼠育种和采购外包给Taconic， 无鼠病原体屏障条件与其大多数商业育种实践相同。Taconic 将使用其传统的递送机制，每两周轮换一次将小鼠递送到所有研究地点， 从而避免长时间的隔离。 目的2：产生新的突变小鼠组合。核心会产生新的变异老鼠 引入不同遗传元件的组合，例如新的Cre菌株。 PPG互动：核心B将广泛支持项目1、项目2和项目3，并与核心互动 提供方便的育种，运输和基因分型数据。核心B也将进行额外的杂交 以生成项目所需的任何应变组合。核心B将与核心A交互，以提供简单的 获取育种、运输和基因分型数据，并进行持续监测。