High-Parameter Cytometry Shared Resource

高参数细胞分析共享资源

• 批准号: 10025014
• 负责人: Christine Beeton
• 金额: $ 14.13万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10025014
• 关键词: Antibodies; Award; Biology; Cancer Center; Cancer Center Support Grant; Cell Communication; Cell Cycle Progression; Cell Separation; Cell Survival; Cells; Cellular Metabolic Process; Chromatin; Consultations; Core Facility; Cytometry; DNA; Data Analyses; Development; Ensure; Equipment; Evolution; Flow Cytometry; Funding; Future; Generations; Genetic Transcription; Goals; Grant; Growth; Hour; Image; Immune; In Situ; Individual; Inflammation; Laboratories; Letters; Life; Magnetism; Malignant Childhood Neoplasm; Malignant Neoplasms; Medicine; Metals; Mutation; Neoplasm Metastasis; Nuclear Receptors; Oncogenes; Peer Review; Phenotype; Preparation; Protocols documentation; Publications; Reagent; Research; Research Design; Research Institute; Research Personnel; Resource Sharing; Resources; Sampling; Services; Signal Pathway; Signal Transduction; Sorting - Cell Movement; Suspensions; Technology; Texas; Time; Tissue Sample; Tissues; Training; Transcriptional Regulation; Translating; Tumor Suppression; Tumor Suppressor Proteins; Work; angiogenesis; anticancer research; base; cancer cell; cancer prevention; cancer stem cell; cancer therapy; cell type; college; cost effective; data management; experience; imaging platform; imaging system; instrument; instrumentation; malignant breast neoplasm; meetings; member; neoplastic cell; new technology; new therapeutic target; novel; novel therapeutics; particle; prevent; programs; ranpirnase; recruit; repaired; submicron; success; tissue culture; tool; transforming virus; tumor; tumor microenvironment

PROJECT SUMMARY: High-Parameter Cytometry Shared Resource (HPCSR) Cytometry is an essential and broadly applicable technology for cancer research to assist with elucidating mechanisms of tumor suppressor and oncogenes, cell-cycle progression, transforming viruses, and to evaluate cancer therapies. It is integral to studies of cancer stem cells, angiogenesis, inflammation, transcriptional regulation in tumor cells, and mechanisms of DNA break and repair. The goal of the High-Parameter Cytometry Shared Resource (HPCSR) is to provide a wide range of Cancer Center investigators with access to cost- effective state-of-the-art instrumentation, expertise and training for their cell sorting, and analysis needs. This technology continues to develop at a rapid pace, especially with the advent of novel tools and reagents, increased computational capacity, and more cost-effective equipment. To keep pace with changing technologies, increased utilization, and growth of users over the past 4 years, the HPCSR has expanded its space with new lab renovations, acquired substantial upgrades of existing equipment, and acquired new major equipment utilizing mass, imaging, high-parameter, and large and small particle cytometry. The main facility is housed in centrally located space and is available to trained users 24 hours a day, 7 days a week by key-card access. Current major instrumentation includes a mass cytometer and imaging system, high-parameter fluorescent cytometer, an imaging cytometer, 4 florescence-activated cell sorters, 7 flow analyzers, and magnetic and large particle cell sorters. The Resource is directed by Dr. Christine Beeton and Mr. Joel Sederstrom, whom both have over 20 years of experience in flow cytometry and is staffed by 5 full-time experienced flow cytometrists who perform operator-assisted cytometry and assist users with data analysis. To ensure optimal use of services, HPCSR provides consultations, group and individually tailored training, and protocols for sample preparation, flow analysis, and cell sorting. This is a heavily used Shared Resource that provided service for 119 Cancer Center investigators during the last year, a substantial increase from 87 users in the first year of the CCSG award. It serves all Programs of the DLDCCC with the Programs in Cell Signaling and Metabolism (CSM), Nuclear Receptor, Transcription and Chromatin Biology (NRTCB), Breast Cancer (BCP), Mechanisms in Cancer Evolution (MCEP), and Pediatric Cancer (PCP) being the biggest users of this Shared Resource. This use translates into an average of 50 publications annually. Future plans include development of new technologies and instrumentation for mass, high-parameter, imaging, large and small particle, and spectral cytometry that are pushing the boundaries of conventional cytometry beyond 50 parameters. Further expansion of space and research staff are required for these new technologies and anticipated continued growth of HPCSR.

项目摘要：高参数细胞计数共享资源 (HPCSR) 细胞计数是癌症研究中一项重要且广泛适用的技术，可帮助阐明 肿瘤抑制和癌基因、细胞周期进展、转化病毒的机制，并评估 癌症疗法。它是癌症干细胞、血管生成、炎症、转录研究中不可或缺的一部分。 肿瘤细胞的调节，以及 DNA 断裂和修复的机制。高参数细胞术的目标 共享资源 (HPCSR) 旨在为广泛的癌症中心研究人员提供获取成本的途径 有效的最先进的仪器、专业知识和培训来满足他们的细胞分选和分析需求。这 技术继续快速发展，特别是随着新型工具和试剂的出现， 更高的计算能力和更具成本效益的设备。为了跟上不断变化的技术， 过去 4 年来，随着利用率的提高和用户的增长，HPCSR 通过新的产品扩展了其空间 实验室改造，对现有设备进行大幅升级，并购置新的主要设备 利用质量、成像、高参数以及大小颗粒细胞计数。主要设施位于 空间位于中心位置，每周 7 天、每天 24 小时可供经过培训的用户通过钥匙卡访问。 目前主要仪器包括质谱流式细胞仪和成像系统、高参数荧光分析仪、 细胞仪、成像细胞仪、4 个荧光激活细胞分选仪、7 个流式分析仪以及磁性和大型细胞分析仪 颗粒细胞分选仪。该资源由 Christine Beeton 博士和 Joel Sederstrom 先生指导，他们都曾 拥有超过20年的流式细胞术经验，并拥有5名经验丰富的全职流式细胞术师 执行操作员辅助的细胞计数并协助用户进行数据分析。为了确保服务的最佳使用， HPCSR 提供​​咨询、团体和单独定制的培训以及样品制备协议、 流式分析和细胞分选。这是一个被大量使用的共享资源，为 119 Cancer 提供服务 去年中心调查员数量较 CCSG 第一年的 87 名用户大幅增加 奖。它服务于 DLDCCC 的所有项目以及细胞信号传导和代谢 (CSM) 项目， 核受体、转录和染色质生物学 (NRTCB)、乳腺癌 (BCP)、癌症机制 Evolution (MCEP) 和儿科癌症 (PCP) 是该共享资源的最大用户。这个用 平均每年出版 50 篇出版物。未来的计划包括开发新技术 以及用于质量、高参数、成像、大颗粒和小颗粒以及光谱细胞计数的仪器 将传统细胞计数技术的界限推向了 50 个参数之外。进一步拓展空间和 这些新技术需要研究人员，并且预计 HPCSR 将持续增长。