Shared Resource: Cytometry and Cell Sorting

细胞计数和细胞分选共享资源（细胞和体内生物学组） 项目摘要 流式细胞术是癌症研究的基本和广泛适用的技术，以帮助阐明 肿瘤抑制和癌基因的机制，细胞周期进程，转化病毒，并评估 癌症治疗它也是研究癌症干细胞、血管生成、炎症、转录和免疫学的重要组成部分。 调节肿瘤细胞和DNA断裂和修复的机制。流式细胞术和细胞计数的目标 分类共享资源（CCSSR）是为广泛的癌症中心研究人员提供访问 具有成本效益的最先进的仪器、专业知识和培训，以满足他们的细胞分选和分析需求。 该技术继续快速发展，特别是随着新工具和试剂的出现， 提高计算能力和更经济的光学设备。为了跟上不断变化 在过去的4年里，随着技术的发展和用户的增长，CCSSR已经通过新的实验室扩大了其空间。 翻修，在我们的附属德克萨斯儿童医院癌症中心增加了一个卫星设施，并收购了 对原有装备进行了大幅升级，并购置了更多新的主要装备。的 主要设施位于中心位置，可供经过培训的用户7天、每天24小时使用， 一周内通过钥匙卡进入。目前的主要仪器包括四个完全荧光激活的细胞分选仪， 六个流动分析仪和基于磁和微流体的细胞分选仪。资源由克莉丝汀博士指导 Beeton和Joel Sederstrom先生分别在流式细胞术方面拥有超过10年和15年的经验， 并配备三名全职经验丰富的流式细胞仪，他们执行操作员辅助细胞仪， 帮助用户进行数据分析。为了确保最佳利用服务，CCSSR提供个别咨询- 量身定制的培训以及样品制备、流动分析和细胞分选的方案。这是一个大量使用的 共享资源在过去一年中为125名癌症中心研究人员提供了服务， 从CCSG奖第一年的83个用户增加。未来的计划包括增加新技术 以及基于图像和质量的细胞计数仪的仪器， 流式细胞术和进一步扩大这些新技术所需的空间和研究人员， 预计CCSSR将继续增长。

CORE: Cytometry and Cell Sorting Shared Resource (Cell and in vivo Biology Group) PROJECT SUMMARY Flow cytometry is an essential and broadly applicable technology for cancer research to assist with elucidating mechanisms of tumor suppressor and oncogenes, cell-cycle progression, transforming viruses, and to evaluate cancer therapies. It is also integral to studies of cancer stem cells, angiogenesis, inflammation, transcriptional regulation in tumor cells and mechanisms of DNA break and repair. The goal of the Cytometry and Cell Sorting Shared Resource (CCSSR) is to provide a wide range of cancer center investigators with access to cost-effective state-of-the-art instrumentation, expertise and training for their cell sorting and analysis needs. This technology continues to develop at a rapid pace, especially with the advent of novel tools and reagents, increased computational capacity and more cost-effective optical equipment. To keep pace with changing technologies and growth of users over the past 4 years, the CCSSR has expanded its space with new lab renovations, added a satellite facility at our affiliated Texas Childrens Hospital Cancer Center and acquired substantial upgrades of previously exsiting equipment and acquired additional new major equipment. The main facility is housed in centrally located space and is available to trained users 24 hours a day, seven days a week by key-card access. Current major instrumentation includes four fully florescence-activated cell sorters, six flow analyzers and magnetic- and microfluidic-based cell sorters. The Resource is directed by Dr. Christine Beeton and Mr. Joel Sederstrom, who have over ten and 15 years of experience in flow cytometry respectively, and is staffed by three full-time experienced flow cytometrists who perform operator-assisted cytometry, and assist users with data analysis. To ensure optimal use of services, CCSSR provides consultations, individually- tailored training, and protocols for sample preparation, flow analysis and cell sorting. This is a heavily used Shared Resource that provided service for 125 Cancer Center investigators during the last year, a substantial increase from 83 users in the first year of the CCSG award. Future plans include addition of new technologies and instrumentation for image- and mass-based cytometry that are pushing the boundaries of conventional flow cytometry and further expansion of space and research staff required for these new technologies and anticipated continued growth of CCSSR.