High-Parameter Cytometry Shared Resource

项目总结：高参数细胞仪共享资源(HPCSR) 细胞学是癌症研究中一项基本的和广泛应用的技术，以帮助阐明 肿瘤抑制和癌基因、细胞周期进展、转化病毒的机制及其评价 癌症疗法。它是癌症干细胞、血管生成、炎症、转录的研究不可或缺的一部分。 肿瘤细胞的调控，以及DNA断裂和修复的机制。高参数细胞术的发展目标 共享资源(HPCSR)旨在为广泛的癌症中心研究人员提供访问成本- 有效的最先进的仪器、专业知识和培训，以满足他们的细胞分选和分析需求。这 技术继续快速发展，特别是随着新工具和试剂的出现， 计算能力增强，设备成本效益更高。为了跟上日新月异的技术， 利用率的提高和用户的增长在过去4年中，HPCSR通过新的 实验室翻新，对现有设备进行实质性升级，并购买新的主要设备 利用质量、成像、高参数、大小颗粒细胞术。主要设施设在 位于中心位置，训练有素的用户一周7天、每天24小时都可以通过钥匙卡进入。 目前的主要仪器包括质量细胞仪和成像系统，高参数荧光 细胞仪，成像细胞仪，4个荧光激活的细胞分选器，7个流动分析仪，以及磁性和大型 粒子细胞分选机。该资源由克里斯汀·比顿博士和乔尔·塞德斯特罗姆先生执导，他们都有 在流式细胞仪领域拥有20多年的经验，拥有5名经验丰富的全职流式细胞仪操作员 执行操作员辅助的细胞分析，并协助用户进行数据分析。为了确保服务的最佳使用， HPCSR提供咨询、小组和个人量身定制的培训，以及样品制备方案， 流动分析和细胞分选。这是一个使用率很高的共享资源，为119癌症提供服务 中心调查人员在去年，与CCSG第一年的87名用户相比大幅增加 获奖。它为DLDCCC的所有程序提供细胞信号和代谢(CSM)程序， 核受体，转录和染色质生物学(NRTCB)，乳腺癌(BCP)，癌症机制 进化(MCEP)和儿科癌症(PCP)是这一共享资源的最大用户。此用途 这相当于平均每年出版50份出版物。未来的计划包括开发新技术 以及用于质量、高参数、成像、大小粒子和光谱细胞术的仪器，这些都是 将传统的细胞学的界限突破了50个参数。空间的进一步扩展和 这些新技术需要研究人员，预计HPCSR将继续增长。

PROJECT SUMMARY: High-Parameter Cytometry Shared Resource (HPCSR) Cytometry is an essential and broadly applicable technology for cancer research to assist with elucidating mechanisms of tumor suppressor and oncogenes, cell-cycle progression, transforming viruses, and to evaluate cancer therapies. It is integral to studies of cancer stem cells, angiogenesis, inflammation, transcriptional regulation in tumor cells, and mechanisms of DNA break and repair. The goal of the High-Parameter Cytometry Shared Resource (HPCSR) is to provide a wide range of Cancer Center investigators with access to cost- effective state-of-the-art instrumentation, expertise and training for their cell sorting, and analysis needs. This technology continues to develop at a rapid pace, especially with the advent of novel tools and reagents, increased computational capacity, and more cost-effective equipment. To keep pace with changing technologies, increased utilization, and growth of users over the past 4 years, the HPCSR has expanded its space with new lab renovations, acquired substantial upgrades of existing equipment, and acquired new major equipment utilizing mass, imaging, high-parameter, and large and small particle cytometry. The main facility is housed in centrally located space and is available to trained users 24 hours a day, 7 days a week by key-card access. Current major instrumentation includes a mass cytometer and imaging system, high-parameter fluorescent cytometer, an imaging cytometer, 4 florescence-activated cell sorters, 7 flow analyzers, and magnetic and large particle cell sorters. The Resource is directed by Dr. Christine Beeton and Mr. Joel Sederstrom, whom both have over 20 years of experience in flow cytometry and is staffed by 5 full-time experienced flow cytometrists who perform operator-assisted cytometry and assist users with data analysis. To ensure optimal use of services, HPCSR provides consultations, group and individually tailored training, and protocols for sample preparation, flow analysis, and cell sorting. This is a heavily used Shared Resource that provided service for 119 Cancer Center investigators during the last year, a substantial increase from 87 users in the first year of the CCSG award. It serves all Programs of the DLDCCC with the Programs in Cell Signaling and Metabolism (CSM), Nuclear Receptor, Transcription and Chromatin Biology (NRTCB), Breast Cancer (BCP), Mechanisms in Cancer Evolution (MCEP), and Pediatric Cancer (PCP) being the biggest users of this Shared Resource. This use translates into an average of 50 publications annually. Future plans include development of new technologies and instrumentation for mass, high-parameter, imaging, large and small particle, and spectral cytometry that are pushing the boundaries of conventional cytometry beyond 50 parameters. Further expansion of space and research staff are required for these new technologies and anticipated continued growth of HPCSR.