High-Parameter Cytometry Shared Resource

高参数细胞计数共享资源（HPCSR） 细胞计数是一种重要的和广泛适用的技术，用于癌症研究，以帮助阐明 肿瘤抑制和癌基因的机制，细胞周期进程，转化病毒，并评估 癌症治疗它是研究癌症干细胞、血管生成、炎症、转录、 肿瘤细胞中的调控，以及DNA断裂和修复的机制。高参数细胞计数的目标 共享资源（HPCSR）是为广泛的癌症中心研究人员提供成本- 有效的最先进的仪器，专业知识和培训，以满足他们的细胞分选和分析需求。这 技术继续快速发展，特别是随着新工具和试剂的出现， 提高计算能力和更经济的设备。为了跟上不断变化的技术， 在过去的4年里，随着利用率的提高和用户的增长，HPCSR通过新的 实验室翻新，对现有设备进行了实质性升级，并购置了新的主要设备 利用质量、成像、高参数和大小颗粒细胞术。主要设施位于 中心位置的空间，并提供给受过培训的用户，每天24小时，每周7天，通过钥匙卡访问。 目前的主要仪器包括质谱流式细胞仪和成像系统、高参数荧光显微镜和荧光显微镜。 流式细胞仪，一个成像细胞仪，4个荧光激活细胞分选仪，7个流式分析仪，以及磁性和大 粒子细胞分选仪。该资源是由克莉丝汀比顿博士和乔尔Sederstrom先生，谁都有 拥有超过20年的流式细胞术经验，并拥有5名全职经验丰富的流式细胞术师， 进行操作员辅助的细胞计数并协助用户进行数据分析。为确保服务得到最佳利用， HPCSR提供咨询、团体和个人定制培训以及样品制备方案， 流动分析和细胞分选。这是一个大量使用的共享资源，为119癌症提供服务 中心研究人员在过去一年中，从87个用户在CCSG的第一年大幅增加 奖它为DLDCCC的所有项目提供细胞信号传导和代谢（CSM）， 核受体，转录和染色质生物学（NRTCB），乳腺癌（BCP），癌症机制 进化（MCEP）和儿科癌症（PCP）是这个共享资源的最大用户。这种使用 转化为平均每年50份出版物。未来的计划包括开发新技术 以及用于质量、高参数、成像、大小颗粒和光谱细胞术的仪器， 将传统细胞计数的界限推到50个参数之外。空间的进一步扩大， 这些新技术和预期的HPCSR的持续增长需要研究人员。

PROJECT SUMMARY: High-Parameter Cytometry Shared Resource (HPCSR) Cytometry is an essential and broadly applicable technology for cancer research to assist with elucidating mechanisms of tumor suppressor and oncogenes, cell-cycle progression, transforming viruses, and to evaluate cancer therapies. It is integral to studies of cancer stem cells, angiogenesis, inflammation, transcriptional regulation in tumor cells, and mechanisms of DNA break and repair. The goal of the High-Parameter Cytometry Shared Resource (HPCSR) is to provide a wide range of Cancer Center investigators with access to cost- effective state-of-the-art instrumentation, expertise and training for their cell sorting, and analysis needs. This technology continues to develop at a rapid pace, especially with the advent of novel tools and reagents, increased computational capacity, and more cost-effective equipment. To keep pace with changing technologies, increased utilization, and growth of users over the past 4 years, the HPCSR has expanded its space with new lab renovations, acquired substantial upgrades of existing equipment, and acquired new major equipment utilizing mass, imaging, high-parameter, and large and small particle cytometry. The main facility is housed in centrally located space and is available to trained users 24 hours a day, 7 days a week by key-card access. Current major instrumentation includes a mass cytometer and imaging system, high-parameter fluorescent cytometer, an imaging cytometer, 4 florescence-activated cell sorters, 7 flow analyzers, and magnetic and large particle cell sorters. The Resource is directed by Dr. Christine Beeton and Mr. Joel Sederstrom, whom both have over 20 years of experience in flow cytometry and is staffed by 5 full-time experienced flow cytometrists who perform operator-assisted cytometry and assist users with data analysis. To ensure optimal use of services, HPCSR provides consultations, group and individually tailored training, and protocols for sample preparation, flow analysis, and cell sorting. This is a heavily used Shared Resource that provided service for 119 Cancer Center investigators during the last year, a substantial increase from 87 users in the first year of the CCSG award. It serves all Programs of the DLDCCC with the Programs in Cell Signaling and Metabolism (CSM), Nuclear Receptor, Transcription and Chromatin Biology (NRTCB), Breast Cancer (BCP), Mechanisms in Cancer Evolution (MCEP), and Pediatric Cancer (PCP) being the biggest users of this Shared Resource. This use translates into an average of 50 publications annually. Future plans include development of new technologies and instrumentation for mass, high-parameter, imaging, large and small particle, and spectral cytometry that are pushing the boundaries of conventional cytometry beyond 50 parameters. Further expansion of space and research staff are required for these new technologies and anticipated continued growth of HPCSR.