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Molecular Pathology and Genomics Core

分子病理学和基因组学核心

基本信息

批准号：
10005248
负责人：
JON C. ASTER
金额：
$ 25.18万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005248
关键词：
Academic Medical Centers Address BCL1 Oncogene BCL2 gene Biological Markers Boston CLIA certified Cancer Center Cells Cities Classification Collection Color Complement Consensus DNA Dana-Farber Cancer Institute Data Data Analyses Development Diagnosis Diagnostic Equipment Evaluation Formalin Functional disorder Genes Genomics Genotype Hematologic Neoplasms Hematopathology Histology Histopathology Hospitals Human IL2RA gene Immune Immune response Immunofluorescence Immunologic In Situ Institution International Knowledge Link Lymphoma MCL1 gene MDM2 gene Medical Genetics Memorial Sloan-Kettering Cancer Center Modeling Mus Mutate Normal tissue morphology North Carolina Paraffin Embedding Pathologic Pathologist Pathology Periodicity Peripheral Primary Neoplasm Protocols documentation Research Research Personnel Resistance Resource Sharing Resources STAT5B gene Sampling Services Site Slide Stains System Systems Analysis T-Cell Lymphoma TP53 gene Testing Tissue Embedding Universities Woman base cohort data analysis pipeline driver mutation experience genomic data imaging platform innovation liquid crystal polymer molecular pathology mouse model neoplastic cell novel novel therapeutics performance site programs reagent standard response success targeted exome sequencing therapeutic target tumor microenvironment web interface web site working group

项目摘要

PROJECT SUMMARY Core C provides access to specialized professional and technical pathology services and genomic services that are essential to the success of the Program. It does so by carrying out 3 overarching aims that support all three P01 Projects: 1) provide pathology expertise needed for classification of primary human PTCLs and PDX and murine PTCL models; 2) perform standard and novel in situ tests for biomarkers relevant to understanding PTCL pathophysiology and its response and resistance to novel therapies; and 3) perform targeted exome sequencing of a large cohort of well-characterized human PTCLs. The Core delivers these services at two performance sites, one in Boston led by Jon Aster, which will deliver services supporting Aims 1 and 2, and the second in North Carolina led by Sandeep Dave, which will deliver services supporting Aims 1 and 3. Delivery of Aim 1 services relies on the Core's co-leader, Dr. Jon Aster, who is a practicing academic hematopathologist with >25 years of experience with human and murine hematopathology, and is supplemented by additional expertise in Project 1 (John Chan) and access to the Hematologic Malignancies Research Consortium, which includes >25 institutions and has accrued over 1800 cases of T cell lymphomas, a majority with paired normal tissue. Aim 2 services will be provided in Boston include standard histology and immunohistology services and innovative, multiparametric immunofluorescence staining platforms, the Perkin- Elmer Vectra platform and CyCIF is an iterative imaging platform that allows for evaluation of >50 different markers on single FFPE slides. Aim 3 services are delivered at the North Carolina performance site by performing targeted exome sequencing on 410 genes that are frequently mutated in lymphoma, initially using DNA prepared from a cohort of 1833 PTCLs and paired normal tissue controls.

项目摘要核心C提供专业的专业和技术病理学服务和基因组服务这对该计划的成功至关重要。它通过实现3个总体目标来实现这一目标，三个P01项目：1）提供原发性人类PTCL和PDX分类所需的病理学专业知识和鼠PTCL模型; 2）对与理解PTCL相关的生物标志物进行标准和新的原位测试， PTCL病理生理学及其对新疗法的反应和抗性;以及3）进行靶向外显子组对大量充分表征的人PTCL进行测序。核心提供这些服务，一个位于波士顿，由乔恩·阿斯特领导，将提供支持目标1和2的服务，第二个在北卡罗来纳州，由Sandeep Dave领导，将提供支持目标1和3的服务。递送 Aim 1服务的核心依赖于核心的共同领导人Jon Aster博士，他是一位实践学者具有>25年人类和鼠血液病理学经验的血液病理学家，补充项目1（John Chan）的额外专业知识，并获得血液肿瘤研究联盟，其中包括>25个机构，并已累计超过1800例T细胞淋巴瘤，大多数是成对的正常组织目标2服务将在波士顿提供，包括标准组织学和免疫组织学服务和创新的多参数免疫荧光染色平台，Perkin- Elmer Vectra平台和CyCIF是一种迭代成像平台，可用于评价>50种不同的单个FFPE载玻片上的标记。Aim 3服务在北卡罗来纳州的演出现场由对淋巴瘤中经常突变的410个基因进行靶向外显子组测序，最初使用从1833例PTCL和配对正常组织对照的队列中制备DNA。