Biological Soft X-ray Tomography at the Advanced Light Source

先进光源下的生物软 X 射线断层扫描

• 批准号: 10037804
• 负责人: Carolyn A Larabell
• 金额: $ 58.9万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2021-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10037804
• 关键词: 3-Dimensional; Algorithmic Software; Biological; Biomedical Research; Calibration; California; Cell Size; Cells; Cellular Structures; Characteristics; Clinical; Collection; Communities; Computer software; Contrast Media; Data; Development; Diabetes Mellitus; Disease; Disseminated Malignant Neoplasm; Educational workshop; Ensure; Feedback; Funding; Goals; Hydration status; Image; Imaging technology; Individual; Internet; Laboratories; Light; Lighting; Maintenance; Mammalian Cell; Methods; Microscope; Modeling; Neurodegenerative Disorders; Organism; Parasites; Peer Review; Preparation; Procedures; Productivity; Research; Research Personnel; Resources; Roentgen Rays; Shapes; Site; Source; Specialist; Specimen; Stains; Synchrotrons; Technology; Training; Training Programs; Work; X-Ray Computed Tomography; X-Ray Tomography; beamline; cellular imaging; computing resources; contrast imaging; cryogenics; data acquisition; design; end of life; experience; human disease; human imaging; improved; insight; instrument; novel therapeutics; operation; outreach; reconstruction; symposium; tomography; tool; user-friendly

Project Summary/Abstract The National Center for X-ray Tomography (NCXT) provides biomedical researchers with access to unique technologies for visualizing and quantifying intact, fully hydrated cells in 3D. The NCXT designed, built, and now operates a soft x-ray microscope (XM-2) at the Advanced Light Source, Berkeley. XM-2 is currently the only microscope of its kind in the Nation. The unique characteristics of soft x-rays make them an excellent illumination source in a microscope designed to images cells. Soft x-rays penetrate large cells easily, allowing researchers to image mammalian cells in great detail. Moreover, soft x-rays produce image contrast without the need to use stains or other contrast agents; procedures that could damage the delicate sub-cellular structures and organization. XM-2 is equipped with a cryogenic specimen stage that can rotate freely through 360 degrees, allowing the specimen to be imaged from different perspectives. The specimen stage allows researchers to image cells by tomographic methods. In tomography 2D images are taken from many different perspectives. The resultant `stack' of projection images is used to calculate a 3D reconstruction of the specimen. In effect, we following the same principles as used in clinical Computed Tomography (CT), but on individual cells rather than a whole body. This funding will support the day-to-day maintenance, upgrades, and user operation of XM-2, together with associated specimen preparation laboratories and ongoing upgrades to our software/computing resources. This support will ensure biomedical researchers have access to a unique cell imaging technology. During the funding cycle, we will achieve the following specific aims: Aim 1: Enable user access to biological soft x-ray tomography (SXT) at the ALS. The work of this aim will ensure users can bring cells to NCXT and leave with soft x-ray tomographic reconstructions of their specimens. Significant consideration will be striving to make the Resource as user-friendly as possible, and therefore accessible to the broadest possible research community as possible, not just to imaging specialists. Aim 2: Maintain and calibrate XM-2, the NCXT operated soft x-ray microscope at the ALS. As with all synchrotron beamlines, XM-2 is a sophisticated instrument that requires regular maintenance and calibration. We will take a programmatic approach to ensure XM-2 is robust, reliable, and operates with minimum unscheduled downtime. Aim 3: Implement upgrades and enhancements to ensure the NCXT remains `state-of-the-art.' Over the timescale of the funding cycles, new hardware, software, and algorithms will become available, and some existing components will reach their end-of-life. We will, therefore, carry out upgrades and enhancements to maintain the NCXT at the `state-of-the-art.'

项目摘要/摘要 国家X射线断层扫描中心（NCXT）为生物医学研究人员提供了独特的访问权限 在3D中可视化和量化完整的完全水合细胞的技术。 NCXT设计，建造和 现在，在伯克利高级光源处操作一个软X射线显微镜（XM-2）。 XM-2当前是 在全国只有同类的显微镜。 软X射线的独特特征使它们成为显微镜设计的极好的照明源 到图像单元格。软X射线容易穿透大细胞，使研究人员可以在大量 细节。此外，软X射线会产生图像对比度，而无需使用污渍或其他对比剂。 可能损害微妙的亚细胞结构和组织的程序。 XM-2配备了 低温标本阶段可以自由旋转360度，从而使样品从 不同的观点。标本阶段允许研究人员通过层析成像方法对细胞进行图像。在 层析成像2D图像是从许多不同的角度拍摄的。产生的投影图像的“堆栈” 用于计算样品的3D重建。实际上，我们遵循与 临床计算机断层扫描（CT），但在单个细胞而不是整个身体上。 这笔资金将支持XM-2的日常维护，升级和用户操作以及 相关的标本准备实验室，并持续升级到我们的软件/计算资源。 这种支持将确保生物医学研究人员可以使用独特的细胞成像技术。在 资金周期，我们将达到以下特定目标： AIM 1：使用户可以在ALS访问生物柔软的X射线断层扫描（SXT）。这个目标的工作将 确保用户可以将细胞带到NCXT，并带有其标本的柔软X射线断层扫描重建。 重要的考虑将努力使资源尽可能友好地使资源，因此 最广泛的研究社区可以访问，而不仅仅是想象专家。 AIM 2：维护和校准XM-2，NCXT在ALS处操作软X射线显微镜。和所有人一样 Synchrotron梁线，XM-2是一种复杂的仪器，需要定期维护和校准。 我们将采用一种程序化方法来确保XM-2坚固，可靠，并且以最少的方式运行 未定的停机时间。 目标3：实施升级和增强功能，以确保NCXT保持“最先进”。在 资金周期，新硬件，软件和算法的时间表将可用，有些 现有组件将达到其寿命。因此，我们将进行升级和增强 将NCXT保持在“最先进的”处。