Biological Soft X-ray Tomography at the Advanced Light Source

先进光源下的生物软 X 射线断层扫描

基本信息

批准号：
10710224
负责人：
Carolyn A Larabell
金额：
$ 88.82万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10710224
关键词：
3-Dimensional Acceleration Adherence Advisory Committees Algorithms Area Beer Biochemical Biological Biomedical Research Biophysics Calibration Cell Size Cells Cellular biology Chemicals Communication Communities Computer software Contrast Media Cryopreservation Data Data Analyses Data Collection Development Diabetes Mellitus Disease Disseminated Malignant Neoplasm Education and Outreach Electron Microscopy Ensure Eukaryotic Cell Feedback Fluorescence Fluorescence Microscopy Funding Future Home Hour Human Image Imaging Techniques Imaging technology Individual Infrastructure Institution Laboratories Laws Length Light Maintenance Microscope Modeling Molecular Machines Monitor Neurodegenerative Disorders Online Systems Organism Parasites Peer Review Persons Preparation Process Productivity Qualifying Questionnaires Reporting Research Research Personnel Resolution Resources Roentgen Rays Running Safety Schedule Services Shapes Site Source Specimen Stains Synchrotrons System Technology Thick Time Training Training Programs Visit Visualization Work X-Ray Tomography absorption aged attenuation base beamline biomedical facility biomedical imaging cell type cellular imaging computerized data processing computing resources contrast imaging cryogenics end of life experience human disease human imaging imaging modality improved insight instrument instrumentation novel therapeutics operation outreach programs reconstruction repaired tomography transmission process ultra high resolution user-friendly

项目摘要

Project Summary/Abstract The National Center for X-ray Tomography (NCXT) is a full-service, user facility for biomedical imaging and research. NCXT Users, whether working on site or remotely, have access to specialized microscopes and imaging techniques, in particular soft x-ray tomography (SXT) and super-resolution cryogenic fluorescence tomography (SR-CFT). In the US, these imaging modalities are only available at the NCXT. SXT microscopes visualize and quantify the mesoscale organization of biological cells, including large eukaryotic cells. No stains or contrast agents are required for SXT imaging. Before being imaged by the NCXT's transmission soft x-ray microscope at the Advanced Light Source, cells are rapidly cryo-preserved; the only pre-imaging step required. SXT is highly complementary to other imaging modalities, particularly fluorescence and electron microscopies. Mesoscale, 3D SXT reconstructions of an entire cell act as a framework for the interpretation of other types of imaging and biophysical data, allowing them to be viewed in the context of the whole cell. XM2 is currently the only microscope of its kind in the Nation. The requested P30 funding will support the day-to-day operation of XM-2, together with the associated user program and specimen preparation laboratories, and a program of upgrades to our software/computing resources. During the funding cycle, we will achieve the following specific aims: Aim 1: Give the biomedical research community access to soft x-ray tomography (SXT). The work of this aim ensures imaging cells by SXT remains an option open to US researchers. Significant consideration will be given to making the Resource as user-friendly as possible, particular towards the community of researchers that work remotely. Aim 2: Maintain and calibrate XM-2, the NCXT operated soft x-ray microscope at the ALS. As with all synchrotron beamlines, XM-2 is a sophisticated instrument that requires regular maintenance and calibration. We will take a programmatic approach to ensure XM-2 is robust, reliable, and operates with minimum unscheduled downtime. Aim 3: Implement upgrades and enhancements to ensure the NCXT remains `state-of-the-art.' Over the timescale of the funding cycle, new hardware, software, and algorithms will become available, and some existing components will reach their end-of-life. We will, therefore, carry out upgrades and enhancements to maintain the NCXT at the `state-of-the-art.'

项目摘要/摘要国家X射线断层扫描中心（NCXT）是一个全方位服务的用户设施生物医学成像和研究。 NCXT用户，无论是在网站上还是远程工作，有权访问专用显微镜和成像技术，特别是软X射线断层扫描（SXT）和超分辨率低温荧光断层扫描（SR-CFT）。在美国，这些成像方式仅在 NCXT。 SXT显微镜可视化和量化生物细胞的中尺度组织，包括大型真核细胞。 SXT不需要污渍或对比剂成像。在由NCXT的传输软X射线显微镜成像之前晚期光源，细胞得到迅速保存；唯一的预示步骤必需的。 SXT高度互补与其他成像方式，尤其是荧光和电子显微镜。中尺度，整个细胞的3D SXT重建作为一个解释其他类型的成像和生物物理数据的框架，允许它们在整个单元格的上下文中查看。 XM2目前是全国唯一的同类显微镜。请求的P30 资金将支持XM-2的日常操作，以及相关的用户程序和标本准备实验室，以及升级程序软件/计算资源。在资金周期中，我们将实现以下内容具体目的：目标1：让生物医学研究社区获得软X射线断层扫描（SXT）。该目标的工作确保SXT成像单元格仍然是对美国研究人员开放的选项。将有重大考虑尽可能用户友好的资源，尤其是针对研究人员社区这项工作遥不可及。 AIM 2：维护和校准XM-2，NCXT运行的软X射线显微镜在ALS。与所有同步束线一样，XM-2是一个复杂的需要定期维护和校准的仪器。我们将接受确保XM-2的程序化方法可靠，可靠，并且以最少的速度运行未定的停机时间。目标3：实施升级和增强功能以确保NCXT 仍然是“最先进的”。在资金周期的时间尺度上，新硬件，软件和算法将可用，一些现有组件将到达他们的终止。因此，我们将进行升级和增强将NCXT保持在“最先进的”处。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Heterochromatin-mediated gene silencing facilitates the diversification of olfactory neurons.

DOI：
10.1016/j.celrep.2014.10.001
发表时间：
2014-11-06
期刊：
Cell reports
影响因子：
8.8
作者：
Lyons DB;Magklara A;Goh T;Sampath SC;Schaefer A;Schotta G;Lomvardas S
通讯作者：
Lomvardas S

Nuclear architecture as an epigenetic regulator of neural development and function.

DOI：
10.1016/j.neuroscience.2014.01.044
发表时间：
2014-04-04
期刊：
NEUROSCIENCE
影响因子：
3.3
作者：
Alexander, J. M.;Lomvardas, S.
通讯作者：
Lomvardas, S.

Enhancer interaction networks as a means for singular olfactory receptor expression.

DOI：
10.1016/j.cell.2014.09.033
发表时间：
2014-10-23
期刊：
Cell
影响因子：
64.5
作者：
Markenscoff-Papadimitriou E;Allen WE;Colquitt BM;Goh T;Murphy KK;Monahan K;Mosley CP;Ahituv N;Lomvardas S
通讯作者：
Lomvardas S

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.

DOI：
10.1016/j.crmeth.2021.100117
发表时间：
2021-11-22
期刊：
Cell reports methods
影响因子：
0
作者：
Loconte V;Chen JH;Cortese M;Ekman A;Le Gros MA;Larabell C;Bartenschlager R;Weinhardt V
通讯作者：
Weinhardt V

Soft X-ray tomograms provide a structural basis for whole-cell modeling.