Biological Soft X-ray Tomography at the Advanced Light Source

先进光源下的生物软 X 射线断层扫描

• 批准号: 10710224
• 负责人: Carolyn A Larabell
• 金额: $ 88.82万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10710224
• 关键词: 3-Dimensional; Acceleration; Adherence; Advisory Committees; Algorithms; Area; Beer; Biochemical; Biological; Biomedical Research; Biophysics; Calibration; Cell Size; Cells; Cellular biology; Chemicals; Communication; Communities; Computer software; Contrast Media; Cryopreservation; Data; Data Analyses; Data Collection; Development; Diabetes Mellitus; Disease; Disseminated Malignant Neoplasm; Education and Outreach; Electron Microscopy; Ensure; Eukaryotic Cell; Feedback; Fluorescence; Fluorescence Microscopy; Funding; Future; Home; Hour; Human; Image; Imaging Techniques; Imaging technology; Individual; Infrastructure; Institution; Laboratories; Laws; Length; Light; Maintenance; Microscope; Modeling; Molecular Machines; Monitor; Neurodegenerative Disorders; Online Systems; Organism; Parasites; Peer Review; Persons; Preparation; Process; Productivity; Qualifying; Questionnaires; Reporting; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Running; Safety; Schedule; Services; Shapes; Site; Source; Specimen; Stains; Synchrotrons; System; Technology; Thick; Time; Training; Training Programs; Visit; Visualization; Work; X-Ray Tomography; absorption; aged; attenuation; base; beamline; biomedical facility; biomedical imaging; cell type; cellular imaging; computerized data processing; computing resources; contrast imaging; cryogenics; end of life; experience; human disease; human imaging; imaging modality; improved; insight; instrument; instrumentation; novel therapeutics; operation; outreach; programs; reconstruction; repaired; tomography; transmission process; ultra high resolution; user-friendly

Project Summary/Abstract The National Center for X-ray Tomography (NCXT) is a full-service, user facility for biomedical imaging and research. NCXT Users, whether working on site or remotely, have access to specialized microscopes and imaging techniques, in particular soft x-ray tomography (SXT) and super-resolution cryogenic fluorescence tomography (SR-CFT). In the US, these imaging modalities are only available at the NCXT. SXT microscopes visualize and quantify the mesoscale organization of biological cells, including large eukaryotic cells. No stains or contrast agents are required for SXT imaging. Before being imaged by the NCXT's transmission soft x-ray microscope at the Advanced Light Source, cells are rapidly cryo-preserved; the only pre-imaging step required. SXT is highly complementary to other imaging modalities, particularly fluorescence and electron microscopies. Mesoscale, 3D SXT reconstructions of an entire cell act as a framework for the interpretation of other types of imaging and biophysical data, allowing them to be viewed in the context of the whole cell. XM2 is currently the only microscope of its kind in the Nation. The requested P30 funding will support the day-to-day operation of XM-2, together with the associated user program and specimen preparation laboratories, and a program of upgrades to our software/computing resources. During the funding cycle, we will achieve the following specific aims: Aim 1: Give the biomedical research community access to soft x-ray tomography (SXT). The work of this aim ensures imaging cells by SXT remains an option open to US researchers. Significant consideration will be given to making the Resource as user-friendly as possible, particular towards the community of researchers that work remotely. Aim 2: Maintain and calibrate XM-2, the NCXT operated soft x-ray microscope at the ALS. As with all synchrotron beamlines, XM-2 is a sophisticated instrument that requires regular maintenance and calibration. We will take a programmatic approach to ensure XM-2 is robust, reliable, and operates with minimum unscheduled downtime. Aim 3: Implement upgrades and enhancements to ensure the NCXT remains `state-of-the-art.' Over the timescale of the funding cycle, new hardware, software, and algorithms will become available, and some existing components will reach their end-of-life. We will, therefore, carry out upgrades and enhancements to maintain the NCXT at the `state-of-the-art.'

项目总结/文摘

项目成果

Heterochromatin-mediated gene silencing facilitates the diversification of olfactory neurons.

• DOI: 10.1016/j.celrep.2014.10.001
• 发表时间: 2014-11-06
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Lyons DB;Magklara A;Goh T;Sampath SC;Schaefer A;Schotta G;Lomvardas S
• 通讯作者: Lomvardas S

Nuclear architecture as an epigenetic regulator of neural development and function.

• DOI: 10.1016/j.neuroscience.2014.01.044
• 发表时间: 2014-04-04
• 期刊: NEUROSCIENCE
• 影响因子: 3.3
• 作者: Alexander, J. M.;Lomvardas, S.
• 通讯作者: Lomvardas, S.

Enhancer interaction networks as a means for singular olfactory receptor expression.

• DOI: 10.1016/j.cell.2014.09.033
• 发表时间: 2014-10-23
• 期刊: Cell
• 影响因子: 64.5
• 作者: Markenscoff-Papadimitriou E;Allen WE;Colquitt BM;Goh T;Murphy KK;Monahan K;Mosley CP;Ahituv N;Lomvardas S
• 通讯作者: Lomvardas S

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.

• DOI: 10.1016/j.crmeth.2021.100117
• 发表时间: 2021-11-22
• 期刊: Cell reports methods
• 作者: Loconte V;Chen JH;Cortese M;Ekman A;Le Gros MA;Larabell C;Bartenschlager R;Weinhardt V
• 通讯作者: Weinhardt V

A protocol for full-rotation soft X-ray tomography of single cells.

• DOI: 10.1016/j.xpro.2022.101176
• 发表时间: 2022-03-18
• 期刊: STAR protocols
• 作者: Chen JH;Vanslembrouck B;Loconte V;Ekman A;Cortese M;Bartenschlager R;McDermott G;Larabell CA;Le Gros MA;Weinhardt V
• 通讯作者: Weinhardt V