Do E-Cigarette Users Airways Have an Altered Lipid Content?

电子烟使用者的呼吸道脂质含量是否发生改变?

基本信息

  • 批准号:
    10037769
  • 负责人:
  • 金额:
    $ 19.44万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-08-01 至 2022-06-30
  • 项目状态:
    已结题

项目摘要

Compelling new data suggests that e-cigarettes are toxic to the lungs. Indeed, there have now been over 700 hospitalization cases due to acute lung injury in vapers. The cause(s) of this injury are poorly understood and whilst many hospitalized vapers said that they have used THC diluted in Vitamin E oil, many did not. We previously collected serum, saliva and sputum from healthy never-smokers, tobacco smokers or vapers. We also performed research bronchoscopies and collected bronchoalveolar lavage fluid (BALF). From these samples, our group found unique changes to the bronchial and secreted proteomes. We also found that vaping increased levels of lung proteases that are known to cause emphysema and bronchiectasis. Our preliminary data indicate that vapers’ macrophages are phenotypically different to non-smokers’ and smokers’ macrophages, have a greater M1/pro-inflammatory phenotype and significant downregulation of gene expression. Moreover, our new data indicate that vapers’ macrophages have increased numbers of intracellular vacuoles, which may be caused by lipid accumulation. Since we see extensive and significant changes in the vaper’s lung samples, even though they are “healthy”, we propose that they are an ideal biobanked data set that can be used to assess the extent to which altered lung lipid content and lipid-laden/foamy macrophages exist. Importantly, since we obtained an NIH certificate of confidentiality, we also know about any potential drug use. We have recently developed assays to detect nicotine in vapers’ BALF and sputum and we also propose to develop our analytic techniques in order to detect THC and its metabolites in these samples, and we will correlate any changes in lung macrophages/lipids with lung nicotine and THC levels. To achieve these goals, we propose the following specific aims: Aim 1. To determine the concentrations of surfactant proteins in biobanked samples of BAL, sputum and saliva from non-smokers, smokers and vapers. Aim 2. To perform lipidomics and stain alveolar macrophages with Oil Red O to look for altered lipid content in vaper’s macrophages and airway secretions. Aim 3. To perform metabolomics on biobanked samples of BAL, sputum and saliva from non-smokers, smokers and vapers. These studies will use biobanked lung samples from a CTP-funded study (P50 HL120100). We believe that this proposal is responsive to the following FDA areas of interest: (i)Examine health outcomes from various tobacco product types, not to include cigarettes; (ii) Examine the long- and short-term health effects of transitioning from combustible to non-combustible tobacco, and from non-combustible to combustible tobacco, using biomarker and/or health outcomes; (iii) Compare biomarker expression profiles with self-reported use of tobacco products, and analyze measures associated with biomarkers of exposure that may inform risk associated with tobacco exposure for mortality/morbidity by age, sex, and race/ethnicity.
令人信服的新数据表明,电子烟对肺部有毒。事实上,现在已经有700多个了

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

ROBERT TARRAN其他文献

ROBERT TARRAN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('ROBERT TARRAN', 18)}}的其他基金

Novel Peptide Fusion Inhibitors for the Treatment of COVID-19
用于治疗 COVID-19 的新型肽融合抑制剂
  • 批准号:
    10379832
  • 财政年份:
    2022
  • 资助金额:
    $ 19.44万
  • 项目类别:
ELD607 Orai1 Antagonist Increases Bacterial Clearance from the Lung
ELD607 Orai1 拮抗剂可增加肺部细菌清除率
  • 批准号:
    10453601
  • 财政年份:
    2020
  • 资助金额:
    $ 19.44万
  • 项目类别:
ELD607 Orai1 Antagonist Increases Bacterial Clearance from the Lung
ELD607 Orai1 拮抗剂可增加肺部细菌清除率
  • 批准号:
    10404327
  • 财政年份:
    2020
  • 资助金额:
    $ 19.44万
  • 项目类别:
Do E-Cigarette Users Airways Have an Altered Lipid Content?
电子烟使用者的呼吸道脂质含量是否发生改变?
  • 批准号:
    10220134
  • 财政年份:
    2020
  • 资助金额:
    $ 19.44万
  • 项目类别:
ELD607 Orai1 Antagonist Increases Bacterial Clearance from the Lung
ELD607 Orai1 拮抗剂可增加肺部细菌清除率
  • 批准号:
    10080273
  • 财政年份:
    2020
  • 资助金额:
    $ 19.44万
  • 项目类别:
Project 1: The Effects of New and Emerging Tobacco Products on Lung Hyd
项目 1:新型和新兴烟草产品对肺水肿的影响
  • 批准号:
    8904703
  • 财政年份:
    2013
  • 资助金额:
    $ 19.44万
  • 项目类别:
The Impact of Tobacco Exposure on the Lungs Innate Defense System
烟草暴露对肺部先天防御系统的影响
  • 批准号:
    8582362
  • 财政年份:
    2013
  • 资助金额:
    $ 19.44万
  • 项目类别:
The Impact of Tobacco Exposure on the Lungs Innate Defense System
烟草暴露对肺部先天防御系统的影响
  • 批准号:
    9328114
  • 财政年份:
    2013
  • 资助金额:
    $ 19.44万
  • 项目类别:
The Impact of Tobacco Exposure on the Lungs Innate Defense System
烟草暴露对肺部先天防御系统的影响
  • 批准号:
    8737945
  • 财政年份:
    2013
  • 资助金额:
    $ 19.44万
  • 项目类别:
Core A: Administration and Bioanalysis Core
核心 A:管理和生物分析核心
  • 批准号:
    8904707
  • 财政年份:
    2013
  • 资助金额:
    $ 19.44万
  • 项目类别:

相似海外基金

The role of alveolar macrophages and regulatory pathways in post-transplant lung inflammation.
肺泡巨噬细胞和调节途径在移植后肺部炎症中的作用。
  • 批准号:
    23K08315
  • 财政年份:
    2023
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
MICA: Key mechanisms underlying inhaled GM-CSF's enhancement of phagocytosis and bacterial clearance by human alveolar macrophages.
MICA:吸入 GM-CSF 增强人肺泡巨噬细胞吞噬作用和细菌清除的关键机制。
  • 批准号:
    MR/X005046/1
  • 财政年份:
    2023
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Research Grant
Analysis of pathogenic alveolar macrophages which release IL-1alpha in response to fine particles.
分析响应细颗粒物释放 IL-1α 的致病性肺泡巨噬细胞。
  • 批准号:
    23H03154
  • 财政年份:
    2023
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Glutamine Metabolism in Alveolar Macrophages following Influenza A Infection
甲型流感感染后肺泡巨噬细胞的谷氨酰胺代谢
  • 批准号:
    10607319
  • 财政年份:
    2023
  • 资助金额:
    $ 19.44万
  • 项目类别:
The function and regulation of tissue resident alveolar macrophages turnover by host and environmental factors during homeostasis and in infections
稳态和感染期间宿主和环境因素对组织驻留肺泡巨噬细胞周转的功能和调节
  • 批准号:
    471247
  • 财政年份:
    2022
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Fellowship Programs
Using a Lung on Chip Device to Study Alveolar Macrophages as Intracellular Reservoirs for Staphylococcus aureus
使用肺芯片装置研究肺泡巨噬细胞作为金黄色葡萄球菌的细胞内储库
  • 批准号:
    485971
  • 财政年份:
    2022
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Studentship Programs
Analysis of innate immune response of alveolar macrophages and epithelial-mesenchymal transition of alveolar epithelial cells
肺泡巨噬细胞的先天免疫反应和肺泡上皮细胞的上皮间质转化分析
  • 批准号:
    22K06698
  • 财政年份:
    2022
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Cholesterol crystal-mediated inflammation in alveolar macrophages: an emerging role inidiopathic lung fibrosis?
胆固醇晶体介导的肺泡巨噬细胞炎症:在特发性肺纤维化中的新兴作用?
  • 批准号:
    462596862
  • 财政年份:
    2021
  • 资助金额:
    $ 19.44万
  • 项目类别:
    WBP Position
Elucidation of idiopathic pneumonia syndrome: Angiotensin 2 activates alveolar macrophages
特发性肺炎综合征的阐明:血管紧张素 2 激活肺泡巨噬细胞
  • 批准号:
    21K16251
  • 财政年份:
    2021
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Establishment of novel macrophage cell lines to study the pathogenesis of respiratory bacterial pathogens in lung alveolar macrophages
建立新型巨噬细胞系以研究肺泡巨噬细胞中呼吸道细菌病原体的发病机制
  • 批准号:
    NC/V001019/1
  • 财政年份:
    2021
  • 资助金额:
    $ 19.44万
  • 项目类别:
    Research Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了