Quantifying Effector Functions of Anti-HIV IgG1 Antibodies In Vivo.

项目概要/摘要 有大量证据表明Fc介导的效应子功能对整体免疫应答的重要性。 抗体在体内的活性。然而，病毒中和与效应功能的相对贡献 抗体的抗病毒作用仍然不确定。我们提出了一系列的实验， 量化Fc介导的效应子功能对抗体总体活性的贡献。定量 实验将在恒河猴中SHIV感染的抗体治疗的背景下进行。这些 免疫学中的基本问题尚未得到解答，由此产生的信息有望 提供了关于疫苗的两个主要特性（中和与效应功能）的重要见解- 诱导的IgG反应联合收割机力量来抵御HIV感染的建立。

PROJECT SUMMARY/ABSTRACT There is an abundance of evidence demonstrating the importance of Fc-mediated effector function to the overall activity of an antibody in vivo. However, the relative contribution of virus neutralization versus effector functions to the antiviral effect of an antibody remains undefined. We have proposed a series of experiments that will quantify the contributions of Fc-mediated effector functions to the overall activity of an antibody. The quantitative experiments will be performed in the setting of antibody treatment of SHIV infection in rhesus macaques. These fundamental questions in immunology have yet to be answered, and the resultant information promises to provide important insights on how the two major properties (neutralization versus effector functions) of a vaccine- induced IgG response combine forces to ward off the establishment of HIV infection.