Quantifying Effector Functions of Anti-HIV IgG1 Antibodies In Vivo.

项目摘要/摘要 有大量证据表明Fc介导的效应器功能对整体 抗体在体内的活性。然而，病毒中和作用相对于效应器功能的相对贡献 对一种抗体的抗病毒作用仍未确定。我们已经提出了一系列实验，将 量化Fc介导的效应器功能对抗体整体活性的贡献。量化的 实验将在恒河猴感染新城疫病毒的抗体治疗环境中进行。这些 免疫学中的基本问题尚未得到回答，由此产生的信息有望 提供关于疫苗的两个主要特性(中和和效应器功能)的重要见解- 诱导的免疫球蛋白应答合力抵御HIV感染的建立。

PROJECT SUMMARY/ABSTRACT There is an abundance of evidence demonstrating the importance of Fc-mediated effector function to the overall activity of an antibody in vivo. However, the relative contribution of virus neutralization versus effector functions to the antiviral effect of an antibody remains undefined. We have proposed a series of experiments that will quantify the contributions of Fc-mediated effector functions to the overall activity of an antibody. The quantitative experiments will be performed in the setting of antibody treatment of SHIV infection in rhesus macaques. These fundamental questions in immunology have yet to be answered, and the resultant information promises to provide important insights on how the two major properties (neutralization versus effector functions) of a vaccine- induced IgG response combine forces to ward off the establishment of HIV infection.