Quantifying Specific Nanoparticle Phenotypes in Complex Biological Fluids by Fluorescence Microfluidic Resistive Pulse Sensing.

通过荧光微流体电阻脉冲传感量化复杂生物流体中的特定纳米颗粒表型。

• 批准号: 10011623
• 负责人: Jean-Luc Fraikin
• 金额: $ 25.2万
• 依托单位: SPECTRADYNE, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011623
• 关键词: Academia; Adopted; Adoption; Benchmarking; Biological; Biological Markers; Biology; Caliber; Cell Culture Techniques; Complex; Computer software; Concentration measurement; Coupled; Custom; Detection; Development; Disease; Evaluation; Feedback; Flow Cytometry; Fluorescence; Gene Transduction Agent; Goals; Heterogeneity; Industrialization; Industry; Knowledge; Label; Liquid substance; Measurement; Measures; Mediating; Methods; Microfluidics; Microscope; Modernization; Optical Methods; Optics; Particle Size; Phase; Phenotype; Physiologic pulse; Plaque Assay; Process; Production; Research; Resolution; Sampling; Scheme; Sensitivity and Specificity; Site; Source; Specificity; Speed; System; Techniques; Technology; Testing; Therapeutic; Time; Titrations; Translating; Viral Vector; Virus; Virus Replication; Work; base; biological systems; complex biological systems; cost; design; extracellular vesicles; gene therapy; in vivo; instrument; instrumentation; interest; light scattering; nanoparticle; nanoscale; new technology; particle; patient safety; phase 1 designs; physical property; prevent; prototype; research and development; tool; vector

PROJECT SUMMARY Extracellular vesicle (EV)-based therapies and vector-mediated gene therapies hold enormous promise as disease therapeutics. Both comprise nanoscale particles 30nm to 300 nm in diameter that are produced in complex and heterogeneous biological systems. To translate these materials into effective and commercially viable products requires accurate measurements of the concentration and size of the particles of interest (e.g., virus or EVs), and of any impurities at all stages of research, development and production. However, accurate tools for quantifying these basic parameters are not currently available. Most available methods are incapable of accurate measurements in the relevant size range and in such complex media as required for these applications. Other methods can take days (e.g., biological titer) and provide little or no information about particle impurities. At best these techniques create a bottleneck by requiring cumbersome and costly pre-measurement purification; at worst their measurements are misinterpreted—with important implications for patient safety. A critical unmet need therefore exists for technology that delivers fast and accurate size and concentration measurements of specific particles in complex biological mixtures. Spectradyne has commercialized Microfluidic Resistive Pulse Sensing (MRPS), an electrical technique that is uniquely suited to analyzing complex heterogeneous samples. MRPS is seeing rapid adoption in industry and academia for quantification of EVs and virus. While MRPS accurately measures all particles in a complex sample, it cannot currently distinguish the particles of interest from other similarly-sized particles in the sample. Spectradyne will develop Fluor-MRPS, a powerful new technology that adds the specificity of single-particle fluorescence measurements to the MRPS platform. This new technology will measure the size, concentration, and phenotype of single particles in solution with unprecedented accuracy, and dramatically reduce the time and cost of producing biologically derived materials such as virus- and EV- based therapeutics. To accomplish these goals, five specific aims will be met in Phase I and II of the project. In the Phase I Aims, a prototype instrument will be produced that is capable of simultaneous MRPS and fluorescence analysis of single particles. Sensitivity and throughput will be benchmarked. In the Phase II Aims, the prototype will be optimized for small particle detection, thoroughly evaluated for specificity, sensitivity, and limit of detection, and deployed for beta testing with end users in the real world. Completion of this work will yield an easy-to-use bench top instrument capable of rapid and accurate size and concentration measurements of both specifically labeled nanoparticle phenotypes and impurities in complex biological media. Fluor-MRPS will deliver significant efficiencies and powerful new capabilities in the development and production of gene therapy vectors and EV-based therapeutics.

项目总结