Expanding xenosurveillance capabilities in central America

扩大中美洲的异种监视能力

• 批准号: 10041581
• 负责人: Gregory David Ebel
• 金额: $ 23.77万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-11 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10041581
• 关键词: Ache; Affect; Africa; Americas; Anger; Animals; Bite; Blood; Blood capillaries; Blood specimen; Central America; Child; Clinical Data; Clinical Medicine; Communicable Diseases; Communities; Culicidae; Data; Detection; Devices; Disease; Domestic Animals; Ebola virus; Emerging Communicable Diseases; Enrollment; Entomology; Environment; Epidemic; Exanthema; Fever; Frequencies; Guatemala; Hate; Health; Home environment; Hour; Household; Human; Laboratories; Laboratory Study; Latin America; Methodology; Methods; Middle East; Middle East Respiratory Syndrome Coronavirus; Midgut; Mind; Monitor; Needles; Pain; Paper; Pathogen detection; Performance; Pilot Projects; Population; Process; Public Health; Quantitative Reverse Transcriptase PCR; RNA; Reporting; Resources; Rural; Sampling; Site; Spottings; Symptoms; Syndrome; System; Temperature; Time; Validation; Venipunctures; Venous blood sampling; Veterinarians; Veterinary Medicine; Viral; Visiting Nurse; Work; Yellow fever virus; Zoonoses; base; burden of illness; cohort; design; field study; health economics; innovation; insight; member; minimally invasive; multidisciplinary; next generation sequencing; pandemic disease; pathogen; prospective; surveillance data; syndromic surveillance; tool; transmission process; virology

This proposal is for an R21, Expanding xenosurveillance capabilities in Central America. The project is a highly multidisciplinary effort that includes collaborators with expertise in virology, public health entomology, surveillance, and human clinical and veterinary medicine. Our study is based on the idea that mosquitoes can serve as surveillance tools because they are essentially autonomous blood sampling devices. Freshly fed mosquitoes are collected from within dwellings and blood is used for surveillance purposes. If successful, our work would have the impact of simplifying certain types of surveillance that require human blood because although most people hate it when they and their children are stuck with needles, they don’t seem to mind mosquitoes being removed from within their homes. In addition, our approach has the potential to make sampling from animals safer by vastly reducing the need for direct contact with fearful and angry animals that bite, kick, etc.. Aim 1 focuses on validating xenosurveillance through a direct comparison to human-based syndromic surveillance. Aim 2 is designed to maintain the scientific rigor of our work by obtaining quantitative estimates of the sensitivity of Cx. quinquefasciatus and Ae. aegypti for xenosurveillance. This proposal leverages significant prior work on xenosurveillance that has focused on An. gambiae in W. Africa, and an ongoing pilot study of xenosurveillance in rural Guatemala.

该提案针对 R21，扩大中美洲的异种监视能力。该项目是一个高度 多学科的努力，包括具有病毒学、公共卫生昆虫学专业知识的合作者， 监测以及人类临床和兽医学。我们的研究基于以下观点：蚊子可以 作为监视工具，因为它们本质上是自主血液采样设备。新鲜喂养 从住宅内收集蚊子，并使用血液进行监测。如果成功的话，我们的 这项工作将会简化某些需要人类血液的监测类型，因为 尽管大多数人讨厌自己和孩子被针扎住，但他们似乎并不介意 蚊子被从他们的家中清除。此外，我们的方法有可能使 通过大大减少与恐惧和愤怒的动物直接接触的需要，更安全地从动物中取样 咬、踢等 目标 1 侧重于通过与人类综合征的直接比较来验证异种监视 监视。目标 2 旨在通过获得以下方面的定量估计来保持我们工作的科学严谨性： Cx 的敏感性。 quinquefasciatus 和 Ae.埃及伊蚊用于异种监视。 该提案利用了先前针对 An 的异种监视方面的重要工作。冈比亚 W. 非洲，以及危地马拉农村地区正在进行的异种监视试点研究。