Expanding xenosurveillance capabilities in central America

扩大中美洲的异种监视能力

• 批准号: 10189517
• 负责人: Gregory David Ebel
• 金额: $ 18.55万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-11 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10189517
• 关键词: Ache; Affect; Africa; Americas; Anger; Animals; Bite; Blood; Blood capillaries; Blood specimen; Central America; Child; Clinical Data; Clinical Medicine; Communicable Diseases; Communities; Culicidae; Data; Detection; Devices; Disease; Domestic Animals; Ebola virus; Emerging Communicable Diseases; Enrollment; Entomology; Environment; Epidemic; Exanthema; Fever; Frequencies; Guatemala; Hate; Health; Home; Hour; Household; Human; Laboratories; Laboratory Study; Latin America; Methodology; Methods; Middle East; Middle East Respiratory Syndrome Coronavirus; Midgut; Mind; Monitor; Needles; Pain; Paper; Pathogen detection; Performance; Pilot Projects; Population; Process; Public Health; Quantitative Reverse Transcriptase PCR; RNA; Reporting; Resources; Rural; Sampling; Site; Spottings; Symptoms; Syndrome; System; Temperature; Time; Validation; Venipunctures; Venous blood sampling; Veterinarians; Veterinary Medicine; Viral; Visiting Nurse; Work; Yellow fever virus; Zoonoses; base; burden of illness; cohort; design; detection limit; emerging pathogen; field study; health economics; innovation; insight; member; minimally invasive; multidisciplinary; next generation sequencing; pandemic disease; pathogen; poor communities; prospective; surveillance data; syndromic surveillance; tool; transmission process; virology

This proposal is for an R21, Expanding xenosurveillance capabilities in Central America. The project is a highly multidisciplinary effort that includes collaborators with expertise in virology, public health entomology, surveillance, and human clinical and veterinary medicine. Our study is based on the idea that mosquitoes can serve as surveillance tools because they are essentially autonomous blood sampling devices. Freshly fed mosquitoes are collected from within dwellings and blood is used for surveillance purposes. If successful, our work would have the impact of simplifying certain types of surveillance that require human blood because although most people hate it when they and their children are stuck with needles, they don’t seem to mind mosquitoes being removed from within their homes. In addition, our approach has the potential to make sampling from animals safer by vastly reducing the need for direct contact with fearful and angry animals that bite, kick, etc.. Aim 1 focuses on validating xenosurveillance through a direct comparison to human-based syndromic surveillance. Aim 2 is designed to maintain the scientific rigor of our work by obtaining quantitative estimates of the sensitivity of Cx. quinquefasciatus and Ae. aegypti for xenosurveillance. This proposal leverages significant prior work on xenosurveillance that has focused on An. gambiae in W. Africa, and an ongoing pilot study of xenosurveillance in rural Guatemala.

这项提案是关于R21，扩大中美洲的异种监视能力。该项目是一个高度 多学科的努力，包括在病毒学，公共卫生昆虫学， 监测以及人类临床和兽医学。我们的研究是基于蚊子可以 因为它们基本上是自动血液取样装置。新鲜喂养的 蚊子是从住所内收集的，血液用于监测目的。如果成功，我们 这项工作将简化某些需要人血的监测类型， 尽管大多数人讨厌自己和孩子被针扎，但他们似乎并不介意 将蚊子从家中清除。此外，我们的方法有可能使 通过大大减少与恐惧和愤怒的动物直接接触的需要，从动物身上取样更安全， 咬、踢等。 目标1的重点是通过与基于人类的综合征的直接比较来验证异种监视 监视目标2的目的是通过获得以下方面的定量估计来保持我们工作的科学严谨性 Cx的敏感性。致倦五叠球菌和致倦五叠球菌。埃及用于异种监视。 该提案利用了之前关于异种监视的重要工作，这些工作集中在An上。gambiae在W. 非洲，以及正在危地马拉农村进行的异种监测试点研究。