Genotyping/Virus Resource Core

基因分型/病毒资源核心

• 批准号: 10013207
• 负责人: DAVID Paul ZENISEK
• 金额: $ 20.26万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10013207
• 关键词: Biological; Cell Differentiation process; Communities; Consultations; Core Facility; Core Grant; Custom; Dependovirus; Equipment; Gene Delivery; Genetic Techniques; Genetically Modified Animals; Genotype; Goals; Human Resources; Infrastructure; Laboratories; Laboratory Personnel; Maintenance; Mediating; Modernization; Molecular; Molecular Biology; Molecular Biology Techniques; Molecular Genetics; Mus; Neurons; Pathogenicity; Persons; Plasmids; Postdoctoral Fellow; Preparation; Procedures; Production; Research; Research Personnel; Resources; Savings; Services; Students; Techniques; Time; Training; Training Support; Training and Infrastructure; Transgenic Animals; Universities; Viral; Viral Vector; Virus; Vision; Vision research; cost; design; experimental study; genetic manipulation; immunogenicity; innovation; laboratory experience; molecular array; novel virus; programs; tool; transgene delivery; transgene expression; vector; virus resource

Genotyping and Virus Production Core Module Abstract The Genotyping and Virus Production Core Module will provide the means to incorporate modern molecular and genetic techniques into the research programs of the entire vision research community at Yale. First, the Module will supply genotyping and virus preparation assistance and services for laboratories working with transgenic animals and/or viral techniques. Second, it provides and maintains a range of molecular biology equipment to be shared among vision researchers and aids investigators by providing a service for those without the facilities for basic or advanced molecular biology techniques. Third, it trains new investigators and students in basic molecular biology techniques, including the use of viral vectors. Viral vectors are powerful tools for gene delivery in terminally differentiated cells. For example, adeno-associated viruses (AAVs) have enjoyed increasingly wide application in vision research for their many favorable features that include the lack of pathogenicity, low immunogenicity, and the ability to maintain long-term transgene expression in neurons. The Genotyping and Virus Production core module will be sufficient and well equipped to provide both virus vector design and virus production to meet a variety of research needs among vision researchers at Yale. Although many of the common viruses can be purchased from viral core facilities outside of Yale, having an in- house capability for virus design and production will significantly enhance vision research by saving time and cost and facilitating more efficient and innovative virus design with the convenience of in-person consultation for specific research needs.

基因分型和病毒生产核心模块摘要 基因分型和病毒生产核心模块将提供整合现代分子生物学的方法。 和遗传技术的研究项目，在耶鲁大学的整个视觉研究社区。一是 该模块将提供基因分型和病毒制备援助和服务的实验室工作， 转基因动物和/或病毒技术。第二，它提供并维护一系列分子生物学 视觉研究人员和艾滋病调查人员之间共享设备， 没有基础或先进的分子生物学技术的设施。第三，培训新的调查人员， 学生在基本的分子生物学技术，包括使用病毒载体。病毒载体是强大的 用于在终末分化细胞中进行基因递送的工具。例如，腺相关病毒（AAV）具有 由于其许多有利的特性，包括缺乏 具有致病性、低免疫原性和在神经元中维持长期转基因表达的能力。 基因分型和病毒生产核心模块将足够并装备精良，以提供病毒 载体设计和病毒生产，以满足耶鲁大学视觉研究人员的各种研究需求。 虽然许多常见的病毒可以从耶鲁大学以外的病毒核心设施购买， 用于病毒设计和生产容纳能力将通过节省时间 成本和促进更有效和创新的病毒设计与方便的人咨询 具体的研究需求。