MOLECULAR BIOLOGY MODULE

分子生物学核心的目的是：i)提供和维护可共享的各种设备 在视觉研究人员中；2)通过为那些没有基本或 先进的分子生物学技术；3)帮助新的研究人员和学生掌握基础分子 生物技术；4)为转基因动物实验室提供基因分型服务。 本模块提供的资源将用于共享现有设备的维护 在整个大学的视觉研究人员中使用，雇用两名技术人员并购买 定量RT-PCR系统，以缓解现有机器因高使用量而产生的压力。有一位技术人员，她说。 Adrienne LaRue Marabelle将在布雷迪纪念实验室(BML)224拥有长凳空间，并将处理 转基因动物的基因分型，并将监督机器人工作站和微阵列核心的使用 设施。Marabelle女士在分子生物学技术方面拥有超过15年的经验，并一直在运行 在过去的10年里，眼科的基因芯片设备。另一名技术人员是斯图尔特。 Steven Viviano将在SHM-B103拥有工作台空间，并将与博士后研究员一起工作并进行培训 研究生学习各种分子技术，包括蛋白质印迹，定量聚合酶链式反应， 免疫组织化学。他还将设计和准备用于电穿孔或病毒转染的质粒 在自己的实验室里不具备这样做能力的视力研究人员。维维亚诺先生的作品 将使用泽尼塞克博士实验室的设备和该部内的核心设施进行 并将由泽尼塞克博士密切监督。 几个视觉实验室在电生理学、解剖学和计算机方面拥有主要的专业知识 技术和核心支持使这些视觉研究人员能够越来越多地使用 分子生物学技术在他们的研究中。这个模块在过去有相当大的用处，并且 今后还会继续这样做。预计克雷尔、麦考密克、田和泽尼塞克博士将制作 基因分型服务的使用，Hoh博士、Rizzolo博士和Zeiss博士将利用微阵列设施，Coca博士- Prados、Crair、Hoh、Rizzolo、Tian和Zenisek将利用布雷迪纪念实验室的核心设施 科卡-普拉多斯、克雷尔、赫赫、里佐洛、田、蔡司和泽尼塞克博士将使用维维亚诺先生的 服务。

The purpose of the molecular biology core is i) to provide and maintain a wide range of equipment to be shared among vision researchers; 2) aid investigators by providing a service for those without the facilities for basic or advanced molecular biology techniques; 3) help new investigators and students acquire basic molecular biological techniques; 4) provide genotyping services for laboratories working with transgenic animals. The resources provided by this module will be applied toward maintenance of existing equipment for shared usage among vision researchers throughout the university, to employ two technical staff and to purchase a quantitative RT-PCR system to relieve pressure on existing machines due to heavy usage. One technician, Ms. Adrienne LaRue Marabelle, will have bench space in Brady Memorial Laboratory (BML) 224 and will handle the genotyping of transgenic animals and will oversee the use of the robotic workstation and microarray core facility. Ms. Marabelle has over 15 years of experience in molecular biology techniques and has been running the microarray facility in the Department of Ophthalmology for the past 10 years. A second technician, Mr. Steven Viviano, will have bench space in SHM-B1O3 and will work with and train post-doctoral fellows and graduate students in various molecular techniques including Western blotting, quantitative PCR, immunohistochemistry. He will also design and prepare plasmids for electroporation or viral transfection for vision research investigators that are not equipped to do so in their own laboratories. The work of Mr. Viviano will be carried out using equipment in the laboratory of Dr. Zenisek and core facilities within the Department of Physiology and will be closely supervised by Dr. Zenisek. Several vision laboratories have primary expertise in electrophysiological, anatomical and computational techniques and core support has been instrumental in enabling these vision researchers to increasingly employ molecular biology techniques in their research. This module has enjoyed considerable usage in the past and will continue to do so in the future. It is anticipated that Drs. Crair, McCormick, Tian and Zenisek will make use of the genotyping service, Drs. Hoh, Rizzolo and Zeiss will make use of the microarray facility, Drs. Coca- Prados, Crair, Hoh, Rizzolo, Tian and Zenisek will make use of core facilities in the Brady Memorial Laboratory building and Drs. Coca-Prados, Crair, Hoh, Rizzolo, Tian, Zeiss and Zenisek will make use of Mr. Viviano's services.