MOLECULAR BIOLOGY MODULE

分子生物学模块

• 批准号: 7286514
• 负责人: DAVID Paul ZENISEK
• 金额: $ 1.31万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286514
• 关键词: Biological; Coca; Computational Technique; Core Facility; Electroporation; Equipment; Future; Genotype; Immunohistochemistry; Laboratories; Maintenance; Molecular; Molecular Biology; Molecular Biology Techniques; Ophthalmology; Physiology; Plasmids; Polymerase Chain Reaction; Purpose; Range; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robotics; Running; Services; Students; System; Techniques; Transfection; Transgenic Animals; Universities; Viral; Vision; Vision research; Western Blotting; Work; design; experience; post-doctoral training; pressure

The purpose of the molecular biology core is i) to provide and maintain a wide range of equipment to be shared among vision researchers; 2) aid investigators by providing a service for those without the facilities for basic or advanced molecular biology techniques; 3) help new investigators and students acquire basic molecular biological techniques; 4) provide genotyping services for laboratories working with transgenic animals. The resources provided by this module will be applied toward maintenance of existing equipment for shared usage among vision researchers throughout the university, to employ two technical staff and to purchase a quantitative RT-PCR system to relieve pressure on existing machines due to heavy usage. One technician, Ms. Adrienne LaRue Marabelle, will have bench space in Brady Memorial Laboratory (BML) 224 and will handle the genotyping of transgenic animals and will oversee the use of the robotic workstation and microarray core facility. Ms. Marabelle has over 15 years of experience in molecular biology techniques and has been running the microarray facility in the Department of Ophthalmology for the past 10 years. A second technician, Mr. Steven Viviano, will have bench space in SHM-B1O3 and will work with and train post-doctoral fellows and graduate students in various molecular techniques including Western blotting, quantitative PCR, immunohistochemistry. He will also design and prepare plasmids for electroporation or viral transfection for vision research investigators that are not equipped to do so in their own laboratories. The work of Mr. Viviano will be carried out using equipment in the laboratory of Dr. Zenisek and core facilities within the Department of Physiology and will be closely supervised by Dr. Zenisek. Several vision laboratories have primary expertise in electrophysiological, anatomical and computational techniques and core support has been instrumental in enabling these vision researchers to increasingly employ molecular biology techniques in their research. This module has enjoyed considerable usage in the past and will continue to do so in the future. It is anticipated that Drs. Crair, McCormick, Tian and Zenisek will make use of the genotyping service, Drs. Hoh, Rizzolo and Zeiss will make use of the microarray facility, Drs. Coca- Prados, Crair, Hoh, Rizzolo, Tian and Zenisek will make use of core facilities in the Brady Memorial Laboratory building and Drs. Coca-Prados, Crair, Hoh, Rizzolo, Tian, Zeiss and Zenisek will make use of Mr. Viviano's services.

分子生物学核心的目的是i）提供和维护广泛的设备供共享 在视觉研究人员中; 2）通过为那些没有基本或基本设施的人提供服务来帮助研究人员 帮助新的研究人员和学生掌握基本的分子生物学技术， 生物技术; 4）为从事转基因动物工作的实验室提供基因分型服务。 本模块提供的资源将用于维护现有设备， 在整个大学的视觉研究人员中使用，雇用两名技术人员，并购买 定量RT-PCR系统，以减轻现有机器的压力，由于大量使用。技术员，女士。 Adrienne LaRue Marabelle将在布雷迪纪念实验室（BML）224拥有工作台空间，并将处理 转基因动物的基因分型，并将监督机器人工作站和微阵列核心的使用 设施。Marabelle女士在分子生物学技术方面拥有超过15年的经验， 在过去的10年里，眼科的微阵列设备。第二个技术员，先生。 Steven Viviano将在SHM-B1 O3拥有工作空间，并将与博士后研究员合作并对其进行培训， 研究生在各种分子技术，包括蛋白质印迹，定量PCR， 免疫组化他还将设计和制备用于电穿孔或病毒转染的质粒， 视觉研究调查人员没有配备在自己的实验室这样做。维维亚诺先生的作品 将使用Zenisek博士实验室的设备和部门内的核心设施进行 并将由Zenisek博士密切监督。 几个视觉实验室在电生理学、解剖学和计算方面拥有主要的专业知识 技术和核心支持有助于使这些视觉研究人员越来越多地采用 分子生物学技术在他们的研究中。这个模块在过去有相当多的使用， 今后将继续这样做。预计Crair、McCormick、Tian和Zenisek博士将使 使用基因分型服务，何博士，里佐洛和蔡司将利用微阵列设施，可口可乐博士， Prados、Crair、Hoh、Rizzolo、Tian和Zenisek将利用布雷迪纪念实验室的核心设施 Coca-Prados、Crair、Hoh、Rizzolo、Tian、Zeiss和Zenisek博士将利用Viviano先生的 服务