MOLECULAR BIOLOGY MODULE

分子生物学核心的目的是i）提供和维护广泛的共享设备 视觉研究人员； 2) 通过为那些没有基本或基本设施的人提供服务来帮助调查人员 先进的分子生物学技术； 3）帮助新研究者和学生获得基本的分子 生物技术； 4）为转基因动物实验室提供基因分型服务。 该模块提供的资源将用于维护现有设备以共享 在整个大学的视觉研究人员中使用，雇用两名技术人员并购买一台 定量 RT-PCR 系统可缓解现有机器因大量使用而产生的压力。技术员一名，女士 Adrienne LaRue Marabelle 将在布雷迪纪念实验室 (BML) 224 拥有长凳空间，并将处理 转基因动物的基因分型，并将监督机器人工作站和微阵列核心的使用 设施。 Marabelle女士在分子生物学技术方面拥有超过15年的经验，并一直在运行 过去 10 年一直在眼科使用微阵列设备。第二位技术员，先生。 Steven Viviano 将在 SHM-B1O3 中拥有工作台空间，并将与博士后研究员一起工作并对其进行培训 各种分子技术的研究生，包括蛋白质印迹、定量 PCR、 免疫组织化学。他还将设计和制备用于电穿孔或病毒转染的质粒 没有能力在自己的实验室进行此类研究的视觉研究人员。维维亚诺先生的作品 将使用 Zenisek 博士实验室的设备和部门内的核心设施进行 生理学博士，并将受到泽尼塞克博士的密切监督。 多个视觉实验室拥有电生理学、解剖学和计算方面的主要专业知识 技术和核心支持有助于使这些视觉研究人员越来越多地使用 他们研究中的分子生物学技术。该模块在过去得到了广泛的使用，并且 今后也会继续这样做。预计博士。克雷尔、麦考密克、田和泽尼塞克将制作 使用基因分型服务，博士。 Hoh、Rizzolo 和 Zeiss 将利用微阵列设备。可口可乐- Prados、Crair、Hoh、Rizzolo、Tian 和 Zenisek 将使用布雷迪纪念实验室的核心设施 大楼和博士。 Coca-Prados、Crair、Hoh、Rizzolo、Tian、Zeiss 和 Zenisek 将利用 Viviano 先生的技术 服务。

The purpose of the molecular biology core is i) to provide and maintain a wide range of equipment to be shared among vision researchers; 2) aid investigators by providing a service for those without the facilities for basic or advanced molecular biology techniques; 3) help new investigators and students acquire basic molecular biological techniques; 4) provide genotyping services for laboratories working with transgenic animals. The resources provided by this module will be applied toward maintenance of existing equipment for shared usage among vision researchers throughout the university, to employ two technical staff and to purchase a quantitative RT-PCR system to relieve pressure on existing machines due to heavy usage. One technician, Ms. Adrienne LaRue Marabelle, will have bench space in Brady Memorial Laboratory (BML) 224 and will handle the genotyping of transgenic animals and will oversee the use of the robotic workstation and microarray core facility. Ms. Marabelle has over 15 years of experience in molecular biology techniques and has been running the microarray facility in the Department of Ophthalmology for the past 10 years. A second technician, Mr. Steven Viviano, will have bench space in SHM-B1O3 and will work with and train post-doctoral fellows and graduate students in various molecular techniques including Western blotting, quantitative PCR, immunohistochemistry. He will also design and prepare plasmids for electroporation or viral transfection for vision research investigators that are not equipped to do so in their own laboratories. The work of Mr. Viviano will be carried out using equipment in the laboratory of Dr. Zenisek and core facilities within the Department of Physiology and will be closely supervised by Dr. Zenisek. Several vision laboratories have primary expertise in electrophysiological, anatomical and computational techniques and core support has been instrumental in enabling these vision researchers to increasingly employ molecular biology techniques in their research. This module has enjoyed considerable usage in the past and will continue to do so in the future. It is anticipated that Drs. Crair, McCormick, Tian and Zenisek will make use of the genotyping service, Drs. Hoh, Rizzolo and Zeiss will make use of the microarray facility, Drs. Coca- Prados, Crair, Hoh, Rizzolo, Tian and Zenisek will make use of core facilities in the Brady Memorial Laboratory building and Drs. Coca-Prados, Crair, Hoh, Rizzolo, Tian, Zeiss and Zenisek will make use of Mr. Viviano's services.