Animal Model and Cell Isolation Core

动物模型和细胞分离核心

• 批准号: 10056027
• 负责人: COLLEEN MARIE CRONIGER
• 金额: $ 18.08万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10056027
• 关键词: Acute; Acute Hepatitis; Alcohols; Animal Model; Animals; Back; Biological Assay; Body Weight; Cell Separation; Cell model; Cells; Chronic; Complex; Consult; Consultations; Cost Savings; Data; Data Analyses; Databases; Development; Eating; Endothelial Cells; Ensure; Equipment and supply inventories; Ethanol; Experimental Designs; Exposure to; Fibrosis; Funding; Genetic; Genetic Models; Goals; Harvest; Hepatic; Hepatic Stellate Cell; Hepatocyte; Human Resources; Immune; In Vitro; Injury; International; Knock-out; Laboratories; Lead; Liver; Maintenance; Metabolic; Modeling; Molecular; Molecular Target; Mus; National Institute on Alcohol Abuse and Alcoholism; Ohio; Pathogenesis; Pilot Projects; Population; Primary Cell Cultures; Procedures; Process; Protocols documentation; Quality Control; Rattus; Research; Research Personnel; Research Project Grants; Research Support; Resources; Rodent; Sampling; Sorting - Cell Movement; Source; Standardization; System; Techniques; Testing; Therapeutic Intervention; Tissue Sample; Tissues; alcohol exposure; alcohol research; alcohol response; animal breeding; biobank; body system; clinically relevant; design; diet-induced obesity; efficacious treatment; experience; experimental study; in vivo Model; innovation; macrophage; member; mouse model; novel; novel therapeutic intervention; response; sound; targeted treatment; tissue injury; treatment strategy

ABSTRACT The overall goal of the Northern Ohio Alcohol Center (NOAC) is to identify specific molecular targets of ethanol- induced damage, as well as understand the complex adaptive and maladaptive responses of cells and systems to that injury. This information will enable us to 1) target therapeutic interventions that will either slow and/or reverse the progression of alcohol-induced tissue injury and 2) develop specific assays that can assess the efficacy of novel therapeutic strategies in relevant clinical populations. NOAC brings together an outstanding team of interdisciplinary investigators. Progress by these investigators into the mechanisms of ethanol-induced tissue injury is supported by the Animal Models and Cell Isolation Core (Animal/Cell Core). The use of standardized protocols for in vivo models of acute and chronic ethanol exposure to rodents, as well as the use of in vitro primary cell cultures isolated from ethanol-exposed animals, is critical to understanding the molecular mechanisms for the pathogenesis of alcohol-induced tissue injury. The purpose of the Animal/Cell Core is to provide expertise in the design and implementation of experiments investigating alcohol-induced tissue injury, as well as standardized protocols and centralized facilities for the exposure of rodents to ethanol, as well as isolation of hepatocytes and non-parenchymal cells from the liver. The Core provides support for Research Components and Pilot Projects supported by NOAC, as well as additional projects funded by NIAAA and other sources to local investigators, as well as investigators nationally and internationally. Personnel experienced in working with rat and murine models of acute and chronic ethanol exposure, as well as isolating parenchymal and non-parenchymal cells from rodents, staff the Animal/Cell Core. The proposed Research Components and Pilot Projects will make use of the Animal/Cell Core. The major goal of the Animal/Cell Core will be to make tissue and cellular samples from control and ethanol-exposed animals available to members of NOAC. The procedures involved are complex and expensive; the availability of centralized facilities will allow rapid access of investigators in NOAC, as well as investigators new to alcohol research, to the tissues and cells needed to test novel and innovative hypotheses without the delay of each PI developing these techniques in each of their own laboratories. The Animal/Cell Core also maintains an extensive biorepository of tissues and cells from ethanol-exposed mice and rats. This biorepository allows for considerable cost savings that result from the shared use of samples between the different members of NOAC. Importantly, the shared use of samples is also leveraged to integrate data from multiple investigators to better understand interactions between cells, tissues and systems in response to ethanol. The combination of our outstanding investigative team and excellent Core resources will lead to key discoveries on mechanisms of alcohol-induced tissue injury and lead to the development of efficacious treatment strategies.

摘要 北方俄亥俄州酒精中心（NOAC）的总体目标是确定乙醇的特定分子靶点， 诱导损伤，以及了解细胞和系统的复杂适应性和适应不良反应 对于这种伤害。这些信息将使我们能够1）针对治疗干预， 逆转酒精诱导的组织损伤的进展，以及2）开发可以评估 新的治疗策略在相关临床人群中的疗效。NOAC汇集了一个杰出的 跨学科的研究团队。这些研究人员对乙醇诱导的细胞凋亡机制的研究进展 组织损伤由动物模型和细胞分离核心（Animal/Cell Core）支持。使用 啮齿动物急性和慢性乙醇暴露体内模型的标准化方案，以及使用 从乙醇暴露的动物中分离的体外原代细胞培养物，对于了解 酒精诱导的组织损伤的发病机制。动物/细胞核心的目的是 提供设计和实施研究酒精引起的组织损伤的实验的专业知识， 以及啮齿动物暴露于乙醇的标准化协议和集中设施，以及 从肝脏分离肝细胞和非实质细胞。核心为研究提供支持 NOAC支持的组成部分和试点项目，以及NIAAA和其他机构资助的其他项目 向当地调查人员以及国内和国际调查人员提供信息来源。有经验的人员 用急性和慢性乙醇暴露的大鼠和小鼠模型，以及分离的实质 和啮齿类动物的非实质细胞组成了动物/细胞核心。拟议的研究组成部分和 试点项目将利用动物/细胞核心。动物/细胞核心的主要目标是使 向NOAC成员提供对照动物和接触乙醇动物的组织和细胞样本。的 所涉及的程序复杂且昂贵;集中设施的可用性将允许快速访问 NOAC的研究人员，以及酒精研究的新研究人员，需要组织和细胞， 测试新的和创新的假设，而不延迟每个PI开发这些技术，在他们的每一个 自己的实验室。动物/细胞核心还保持了广泛的组织和细胞的生物储存库， 乙醇暴露的小鼠和大鼠。这种生物储存库允许相当大的成本节省，这是由于 NOAC的不同成员之间共享样本。重要的是，样本的共享使用也是 利用来自多个研究者的数据，更好地了解细胞、组织和细胞之间的相互作用。 和系统对乙醇的反应。我们优秀的调查团队和优秀的核心团队 资源将导致酒精诱导的组织损伤机制的关键发现，并导致 制定有效的治疗策略。