Core B: Animal Models and Cell Isolation Core

核心 B：动物模型和细胞分离核心

• 批准号: 8977735
• 负责人: COLLEEN MARIE CRONIGER
• 金额: $ 17.08万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-15 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8977735
• 关键词: Acute; Acute Hepatitis; Alcohols; Animal Model; Animals; Biological Assay; Biological Markers; Body Weight; Cell Separation; Cell model; Cells; Chronic; Clinical; Complex; Consult; Cost Savings; Data; Databases; Development; Diet; Eating; Endothelial Cells; Ensure; Equipment and supply inventories; Ethanol; Experimental Designs; Fibrosis; Funding; Goals; Harvest; Hepatic Stellate Cell; Hepatocyte; Human Resources; In Vitro; Injury; International; Kupffer Cells; Laboratories; Lead; Metabolic; Modeling; Molecular; Molecular Target; Mus; National Institute on Alcohol Abuse and Alcoholism; Obesity; Ohio; Pathogenesis; Pilot Projects; Population; Primary Cell Cultures; Procedures; Process; Protocols documentation; Rattus; Research; Research Personnel; Research Project Grants; Research Support; Resources; Rodent; Sampling; Source; System; Techniques; Testing; Therapeutic Intervention; Tissue Sample; Tissues; Work; alcohol exposure; alcohol research; biobank; experience; in vivo Model; innovation; member; mouse model; novel; novel therapeutics; oxidation; response; targeted treatment; treatment strategy

The overall goal of the Northern Ohio Alcohol Center (NOAC) is to identify specific molecular targets of ethanol-induced damage, as well as understand the complex adaptive and maladaptive responses of cells and systems to that injury. This information will enable us to 1) target therapeutic interventions that will either slow and/or reverse the progression of alcohol-induced tissue injury and 2) develop specific assays that can assess the efficacy of novel therapeutic strategies in relevant clinical populations. NOAC brings together an outstanding team of interdisciplinary investigators including geneticists, cell biologists, oxidation biochemists, biomarker experts, synthetic chemists and clinical translational investigators. Progress by these investigators into the mechanisms of ethanol-induced tissue injury is supported by the Animal Models and Cell Isolation Core. The use of standardized protocols for in vivo models of acute and chronic ethanol exposure to rodents, as well as the use of in vitro primary cell cultures isolated from ethanol-exposed animals, is critical to understanding the molecular mechanisms for the pathogenesis of alcohol-induced tissue injury. The purpose of the Animal Model and Cell Isolation Core is to provide centralized facilities for the exposure of rats and mice to ethanol in support of Research Components and Pilot Projects supported by NOAC, as well as additional projects funded by NIAAA and other sources. We also provide access to our animal/cell models and biorepository as a national and international resource. This Core also uses standardized protocols for the isolation of hepatocytes and non-parenchymal cells from the liver, providing these cells in support of both NOAC and other alcohol and non-alcohol related research projects. Personnel experienced in working with rat and murine models of acute and chronic ethanol exposure, as well as isolating parenchymal and non- parenchymal cells from rodents, staff the Animal Models and Cell Isolation Core. The proposed Research Components and Pilot Projects will make use of the Animal Models and Cell Isolation Core. The major goal of the Animal Models and Cell Isolation Core will be to make available to members of NOAC tissue and cellular samples from control and ethanol-exposed animals. The procedures involved are complex and expensive; the availability of centralized facilities will allow rapid access of investigators in NOAC, as well as investigators new to alcohol research, to the tissues and cells needed to test novel and innovative hypotheses without the delay of each PI developing these techniques in each of their own laboratories. The Animal Core also maintains an extensive biorepository of tissues and cells from ethanol-exposed mice and rats; this biorepository will allow for considerable cost savings that result from the shared use of samples between the different members of NOAC. The combination of our outstanding investigative team and excellent Core resources will lead to key discoveries on mechanisms of alcohol-induced tissue injury and lead to the development of efficacious treatment strategies

北方俄亥俄州酒精中心（NOAC）的总体目标是确定特定的分子靶点， 乙醇引起的损伤，以及了解细胞的复杂适应性和适应不良反应， 系统对这种伤害。这些信息将使我们能够1）针对治疗干预， 和/或逆转酒精诱导的组织损伤的进展，以及2）开发可以评估 新的治疗策略在相关临床人群中的疗效。NOAC汇集了 杰出的跨学科研究团队，包括遗传学家，细胞生物学家，氧化生物化学家， 生物标志物专家、合成化学家和临床转化研究者。这些调查人员的进展 乙醇诱导的组织损伤的机制得到动物模型和细胞分离的支持 核心啮齿动物急性和慢性乙醇暴露体内模型的标准化方案的使用， 以及使用从乙醇暴露动物中分离的体外原代细胞培养物， 了解酒精诱导的组织损伤发病机制的分子机制。目的 动物模型和细胞分离中心的核心是为大鼠和小鼠的暴露提供集中设施 乙醇，以支持NOAC支持的研究组成部分和试点项目，以及其他 由NIAAA和其他来源资助的项目。我们还提供我们的动物/细胞模型， 生物资源作为国家和国际资源。该核心还使用标准化协议， 从肝脏中分离肝细胞和非实质细胞，提供这些细胞支持肝细胞和非实质细胞。 NOAC和其他酒精和非酒精相关的研究项目。有工作经验的人员 急性和慢性乙醇暴露的大鼠和小鼠模型，以及分离的实质和非实质性模型， 从啮齿类动物的实质细胞，工作人员的动物模型和细胞分离的核心。拟议研究 组件和试点项目将利用动物模型和细胞分离核心。的主要目标 动物模型和细胞分离核心将提供给NOAC组织和细胞的成员， 对照组和乙醇暴露组动物的样本。所涉程序复杂而昂贵; 集中设施的可用性将使NOAC的调查人员以及新的调查人员能够快速访问 到酒精研究，到测试新颖和创新假设所需的组织和细胞， 每个PI在他们自己的实验室开发这些技术。动物中心还保持着 来自乙醇暴露小鼠和大鼠的组织和细胞的广泛生物储存库;该生物储存库将允许 NOAC的不同成员之间共享样品，节省了大量成本。 我们优秀的调查团队和优秀的核心资源相结合，将导致关键的 酒精诱导的组织损伤机制的发现，并导致开发有效的 治疗策略