Characterization of virulence factors in shigellosis

摘要 肠道病原体福氏志贺氏菌是细菌性痢疾(血性腹泻)的病原体， 每年造成2.5亿以上的痢疾病例，造成20多万人死亡。一个 抗击细菌性痢疾的主要挑战是缺乏一种可概括的小动物模型。 在感染者身上观察到的症状。我们团队最近开发了一种独特的细菌模型 幼兔的痢疾。在这个模型中，感染福氏志贺氏菌的动物经历了大量的上皮细胞 开窗、血管损伤、免疫细胞渗入和血性腹泻。而细菌因素 支持福氏志贺氏菌的细胞内感染主要是通过组织培养系统确定的，我们 对导致感染者致病的毒力决定因素知之甚少。在这 应用：我们建议将幼兔模型与转座子测序方法结合使用。 鉴定和鉴定支持细菌性痢疾的细菌因素。

Abstract The intestinal pathogen Shigella flexneri is the causative agent of bacillary dysentery (bloody diarrhea) and is responsible for more than 250 million cases of dysentery annually, resulting in more than 200,000 deaths. A major challenge in combating bacillary dysentery has been the lack of a small-animal model that recapitulates the symptoms observed in infected individuals. Our group has recently developed a unique model of bacillary dysentery in infant rabbits. In this model, animals infected with S. flexneri experience massive epithelial cell fenestration, vascular lesions, immune cell infiltration, and bloody diarrhea. While the bacterial factors supporting S. flexneri intracellular infection have been chiefly determined using tissue culture systems, we know very little about the virulence determinants leading to pathogenesis in infected individuals. In this application, we propose to use the infant rabbit model in conjunction with Transposon-sequencing approaches to identify and characterize the bacterial factors supporting bacillary dysentery.