Genome Analysis Shared Resource

基因组分析共享资源项目摘要 基因组分析共享资源（GEN）为马约诊所癌症中心（MCCC）成员提供 高效、高质量、具有成本效益地获得最先进的基因组学技术， 结构和功能。自2003年以来，GEN各组成部分一直以协调的方式工作， 为核酸测序、基因测序、 表达谱和基因分型。支持NextGen测序和基因表达调控， 2007年增加了RNAi分析。该设施提供的服务包括以下方面的深度测序研究： Illumina NovaSeq、HiSeq和MiSeq仪器以及Pacific Biosciences Single Molecule上的RNA和DNA 新Sequel平台上的真实的时间排序。此外，我们还提供全方位的基因分型和基因 使用Affyphase Genechip™技术和Illumina BeadChips进行转录物分析。甲基化分析是 使用Illumina甲基化阵列和Illumina HiSeq上的减少的代表性亚硫酸氢盐测序提供 仪器. RNA干扰共享资源提供了一个重要的附加功能， 为慢病毒生产提供包装试剂的特殊合同定价， 向所有马约诊所研究者开放Biosystems pGIPZ敲除文库。四个重点领域 （测序、基因表达、基因分型和RNA干扰）由一名高级助理指导 在重点领域具有专门知识的主任。在机构的大力支持下，我们继续增加新的 支持MCCC活动的仪器、人员和服务。有一个发达的合作社 一个管理结构，监督我们的活动，并确保及时获得MCCC和负担得起的价格 成员我们所有的活动都是数据密集型的，我们与IT和生物信息学密切互动 从项目开始到最终交付数据，我们将提供充足的资源来满足用户的需求。以来 上一次竞争性资助更新，来自10个项目和所有3个MCCC站点的158名MCCC成员 使用GEN和MCCC项目提供的服务的人约占该地区所有活动的一半。 将军这一资源对MCCC的价值突出表现在大量高质量、经同行评审的 这些出版物是基于我们的设施产生的数据。

GENOME ANALYSIS SHARED RESOURCE PROJECT SUMMARY The Genome Analysis Shared Resource (GEN) provides Mayo Clinic Cancer Center (MCCC) members efficient, high-quality, cost-effective access to state-of-the-art genomics technologies for studying gene structure and function. Since 2003, the GEN components have been working in a coordinated fashion to provide technical expertise, instrumentation, and comprehensive support for nucleic acid sequencing, gene expression profiling, and genotyping. Support for NextGen sequencing and gene expression regulation through RNAi analysis were added in 2007. The services provided by the facility include deep sequencing studies of RNA and DNA on Illumina NovaSeq, HiSeq and MiSeq instruments, and Pacific Biosciences Single Molecule Real Time Sequencing on the new Sequel platform. In addition, we offer full-service genotyping and gene transcript analysis using Affymetrix Genechip™ technology and Illumina BeadChips. Methylation analyses are offered using Illumina methylation arrays and reduced representational bisulfite sequencing on Illumina HiSeq instruments. An important additional capability is offered by the RNA Interference shared resource, which provides special contract pricing on packaging reagents for lentiviral production and access to the complete Open Biosystems pGIPZ knockdown library to all Mayo Clinic investigators. Each of the 4 areas of focus (Sequencing, Gene Expression, Genotyping and RNA Interference) is directed by a senior staff-level Associate Director with expertise in the area of focus. With significant institutional support, we have continued to add new instruments, personnel and services in support of MCCC activities. There is a well-developed cooperative administrative structure that oversees our activities and ensures timely access and affordable pricing to MCCC members. All of our activities are data intensive, and we interact closely with the IT and bioinformatics resources to meet the needs of our users, from project inception through the final delivery of data. Since the last competitive grant renewal, more than 158 MCCC members from the 10 Programs and all 3 MCCC sites have used the services offered by the GEN and MCCC projects account for about half of all the activity in the GEN. The value of this resource to the MCCC is highlighted by the large number of high-quality, peer-reviewed publications that were based upon data produced in our facilities.