Genome Analysis Shared Resource

基因组分析共享资源项目摘要 基因组分析共享资源(GEN)为梅奥临床癌症中心(MCCC)成员提供 高效、高质量、经济高效地获得用于研究基因的最先进的基因组学技术 结构和功能。自2003年以来，Gen组件一直在以协调的方式工作，以 为核酸测序、基因测序提供技术专长、仪器设备和全面支持 表达谱分析和基因分型。通过以下方式支持下一代测序和基因表达调控 RNAi分析是在2007年添加的。该设施提供的服务包括深度测序研究 Illumina NovaSeq、HiSeq和MiSeq仪器上的RNA和DNA以及太平洋生物科学单分子 在新的Sequel平台上进行实时排序。此外，我们还提供全方位的基因分型和基因检测服务 用Affymetrix基因芯片™技术和Illumina BeadChips进行转录分析甲基化分析是 在Illumina HiSeq上使用Illumina甲基化阵列和减少代表性的亚硫酸氢盐测序 乐器。RNA干扰共享资源提供了一个重要的附加能力， 为慢病毒生产的包装试剂提供特殊合同定价并获得完整的 向所有梅奥诊所的研究人员开放生物系统pGIPZ基因敲除文库。四个重点领域中的每一个 (测序、基因表达、基因分型和RNA干扰)由一名高级参谋级助理指导 在重点领域具有专业知识的董事。在重要的机构支持下，我们继续增加新的 支持MCCC活动的工具、人员和服务。有一个发展很好的合作社 监督我们的活动并确保及时访问MCCC并支付得起价格的行政结构 会员。我们的所有活动都是数据密集型的，我们与IT和生物信息学密切互动 资源，以满足我们用户的需求，从项目开始到数据的最终交付。自.以来 最近一次竞争性资助金续签，来自10个计划和所有3个MCCC站点的158多名MCCC成员 使用了Gen和MCCC项目提供的服务约占 将军。这一资源对MCCC的价值通过大量高质量的、同行审查的 基于我们设施中产生的数据的出版物。

GENOME ANALYSIS SHARED RESOURCE PROJECT SUMMARY The Genome Analysis Shared Resource (GEN) provides Mayo Clinic Cancer Center (MCCC) members efficient, high-quality, cost-effective access to state-of-the-art genomics technologies for studying gene structure and function. Since 2003, the GEN components have been working in a coordinated fashion to provide technical expertise, instrumentation, and comprehensive support for nucleic acid sequencing, gene expression profiling, and genotyping. Support for NextGen sequencing and gene expression regulation through RNAi analysis were added in 2007. The services provided by the facility include deep sequencing studies of RNA and DNA on Illumina NovaSeq, HiSeq and MiSeq instruments, and Pacific Biosciences Single Molecule Real Time Sequencing on the new Sequel platform. In addition, we offer full-service genotyping and gene transcript analysis using Affymetrix Genechip™ technology and Illumina BeadChips. Methylation analyses are offered using Illumina methylation arrays and reduced representational bisulfite sequencing on Illumina HiSeq instruments. An important additional capability is offered by the RNA Interference shared resource, which provides special contract pricing on packaging reagents for lentiviral production and access to the complete Open Biosystems pGIPZ knockdown library to all Mayo Clinic investigators. Each of the 4 areas of focus (Sequencing, Gene Expression, Genotyping and RNA Interference) is directed by a senior staff-level Associate Director with expertise in the area of focus. With significant institutional support, we have continued to add new instruments, personnel and services in support of MCCC activities. There is a well-developed cooperative administrative structure that oversees our activities and ensures timely access and affordable pricing to MCCC members. All of our activities are data intensive, and we interact closely with the IT and bioinformatics resources to meet the needs of our users, from project inception through the final delivery of data. Since the last competitive grant renewal, more than 158 MCCC members from the 10 Programs and all 3 MCCC sites have used the services offered by the GEN and MCCC projects account for about half of all the activity in the GEN. The value of this resource to the MCCC is highlighted by the large number of high-quality, peer-reviewed publications that were based upon data produced in our facilities.