Gene Analysis Shared Resource

基因分析共享资源（GASR）为马约诊所癌症研究人员提供了高效，高质量， 具有成本效益地获得最先进的基因组学技术，用于研究基因结构， 功能自2003年以来，全球安全战略各组成部分一直以协调一致的方式开展工作， 核酸测序、基因表达、基因测序、基因 分析和基因分型。通过RNAi支持NextGen测序和基因表达调控 2007年增加了分析。该设施提供的服务包括RNA的深度测序研究 和DNA，以及Pacific Biosciences单分子真实的时间 测序仪此外，我们还提供使用ABI 3730x1测序仪的全方位测序服务， 基因分型和基因转录物分析使用Affyphase Genechip™技术和Illumina BeadChips。 使用Illumina甲基化阵列和减少的代表性亚硫酸氢盐提供甲基化分析。 在Illumina HiSeq仪器上测序。RNA提供了一个重要的附加功能， 干扰共享资源，提供慢病毒包装试剂的特殊合同定价 所有马约的研究者都可以获得完整的开放生物系统pGIPZ敲低文库。 四项活动中的每一项都由一名在重点领域具有专门知识的高级工作人员级别的共同主任指导。 在重要的机构支持下，我们继续增加新的工具、人员和服务， 支持癌症中心的活动。有一个完善的合作社行政结构， 监督我们的活动，并确保癌症中心成员及时获得和负担得起的价格。所有 我们的活动是数据密集型的，我们与IT和生物信息学资源密切互动，以满足 从项目开始到最终数据交付，我们始终满足用户的需求。自从上一次竞争性拨款以来 更新后，来自所有10个项目的130多名癌症中心成员使用了 GASR和癌症中心项目占GASR所有活动的三分之二左右。 对《全球战略研究报告》的机构支持是例外的，包括为新的文书和 人员来管理他们。这一资源对癌症中心的价值突出表现在大量的 高质量的、同行评审的出版物，这些出版物基于我们设施中产生的数据。

The Gene Analysis Shared Resource (GASR) provides Mayo Clinic cancer researchers efficient, high-quality, cost-effective access to state-of-the-art genomics technologies for studying gene structure and function. Since 2003, the GASR components have been working in a coordinated fashion to provide technical expertise, instrumentation, and coordinated support for nucleic acid sequencing, gene expression profiling, and genotyping. Support for NextGen sequencing and gene expression regulation through RNAi analysis were added in 2007. The services provided by the facility include deep sequencing studies of RNA and DNA on Illumina HiSeq and MiSeq instruments, and a Pacific Biosciences Single Molecule Real Time Sequencer. In addition, we offer full-service sequencing using ABI 3730x1 sequencers, plus comprehensive genotyping and gene transcript analysis using Affymetrix Genechip™ technology and Illumina BeadChips. Methylation analyses are offered using Illumina methylation arrays and reduced representational bisulfite sequencing on Illumina HiSeq instruments. An important additional capability is offered by the RNA Interference Shared Resource, which offers special contract pricing on packaging reagents for lentiviral production and access to the complete Open Biosystems pGIPZ knockdown library to all Mayo investigators. Each of the four activities is directed by a senior, staff-level Co-Director with expertise in the area of focus. With significant institutional support, we have continued to add new instruments, personnel and services in support of Cancer Center activities. There is a well-developed cooperative administrative structure that oversees our activities and ensures timely access and affordable pricing to cancer center members. All of our activities are data intensive, and we interact closely with the IT and bioinformatics resources to meet the needs of our users, from project inception through the final delivery of data. Since the last competitive grant renewal, more than 130 Cancer Center members from all 10 programs have used the services offered by the GASR and Cancer Center projects account for about two-thirds of all the activity in the GASR. Institutional support for GASR has been exceptional, including funding for new instruments and the personnel to run them. The value of this resource to the Cancer Center is highlighted by the large number of high-quality, peer-reviewed publications that were based upon data produced in our facilities.