Small Animal Imaging Lab Core

项目摘要 小动物成像实验室（SAIL）核心设施的目标是提供最先进的成像资源 感谢莫菲特癌症中心（MCC）成员对啮齿动物癌症的基础和转化临床前研究 模型SAIL已扩大其服务范围，提供广泛的多模态成像，包括MRI， 超极化MRI、CT、PET、SPECT、β粒子、超声、生物发光和荧光成像。 这些系统允许成员跟踪肿瘤的发展、进展、转移和对化疗的反应。 在动物模型中使用定量成像进行治疗。SAIL提供高空间分辨率的探测， 许多功能、代谢和解剖学变化，包括缺氧、pH、对细胞的时间敏感性 密度、血流量以及葡萄糖摄取和代谢。这些参数可以使用SAIL的 在图像特征提取和分析方面的专业知识，以生成原位肿瘤生物学的综合分析。 动物肿瘤模型对于理解癌症的生物学和不同肿瘤细胞的复杂反应至关重要。 肿瘤类型进行治疗。对这些动物受试者进行成像是可以精确定义癌症的核心技术 行为。此外，由于SAIL提供的大多数这些模式在诊所中可用， 动物肿瘤模型可以容易地转化为临床试验和临床实践。多模态成像是 该设施的主要重点，因为它提供了广泛的技术，帮助成员的基本 和临床前研究项目。在下一个资助期内，SAIL核心的具体目标是： 目标1。协助成员进行实验设计、结果解释、论文撰写和资助 准备. 目标二。提供和扩展体内和体外成像和分析技术，用于研究 小动物癌症模型 目标3。提供有关小动物成像技术的培训和教育机会， 成员的方法。 在上一个奖项期间，SAIL为三个项目的成员提供服务，并为45个项目做出了贡献。 出版物。在最近的一个财政年度，SAIL为21个成员提供服务，同行评议占总使用量的87%- 资助成员。SAIL使用实验室信息管理系统（LIMS）来整合使用 跟踪、调度和计费功能。LIMS还为项目和数据提供了一个安全的存储库 管理，成员及其实验室工作人员可以访问。

PROJECT SUMMARY The goal of the Small Animal Imaging Lab (SAIL) Core Facility is to provide state-of-the art imaging resources to Moffitt Cancer Center (MCC) members for their basic and translational preclinical studies of rodent cancer models. The SAIL has expanded its services to offer a wide array of multimodality imaging, including MRI, hyperpolarized MRI, CT, PET, SPECT, beta particle, ultrasound, bioluminescence, and fluorescence imaging. These systems allow members to follow tumor development, progression, metastasis and the response to therapy in animal models using quantitative imaging. SAIL provides detection at high spatial resolution of a number of functional, metabolic and anatomical changes, including hypoxia, pH, temporal sensitivity to cellular density, blood flow, and glucose uptake and metabolism. These parameters can be quantified using SAIL's expertise in image feature extraction and analysis to generate an integrated analysis of tumor biology in situ. Animal tumor models are critical for understanding the biology of cancer and the complex responses of distinct tumor types to therapy. Imaging of these animal subjects is a core technology that can precisely define cancer behaviors. Further, as most of these modalities provided by the SAIL are available in the clinic, results with animal tumor models can be readily translated into clinical trials and clinical practice. Multimodality imaging is the key focus of the facility as this provides a broad range of technologies that assist members with their basic and pre-clinical research programs. Over the next funding period, the Specific Aims of the SAIL Core are to: Aim 1. Assist members in experimental design, interpretation of results, and manuscript and grant preparation. Aim 2. Provide and expand in vivo and ex vivo imaging and analytical technologies for research involving small animal cancer models. Aim 3. Provide training and educational opportunities regarding small animal imaging technologies and approaches for members. During the previous award period, SAIL served members from three programs and contributed to 45 publications. In the most recent fiscal year, SAIL served 21 members, with 87% of total usage by peer-review- funded members. The SAIL uses a Laboratory Information Management System (LIMS) to consolidate usage tracking, scheduling, and billing functions. The LIMS also provides a secure repository for project and data management, which is accessible by members and their laboratory staff.