Molecular Biology

项目总结 分子生物学(MB)核心是为了满足对高级分子生物学支持的需求而创建的 被PJI。特别是，利用先进分子生物学的机会和经验对大多数人来说是有限的 PJIS和NIH资助的现有调查人员。具体地说，MB Core将利用和扩展资源 基因实验室核心设施由三个现有的核心设施组成，由 路易斯安那州立大学兽医学院生物技术与分子医学系 (支持向量机)：基因表达和测序实验室(GeneLAB)，蛋白质表征和纯化 实验室(PCPL)，它也负责产生针对感兴趣蛋白质的单特异性抗体， 和分子免疫病理学实验室(MIPL)，提供专业知识和访问生物成像和 先进的免疫荧光和电子显微镜。这三个实验室目前是共同支持的 由NIGMS资助的CEIDR-COBRE(第三阶段)以及路易斯安那州生物医学研究网络 (LBRN/INBRE)。肺眼镜蛇的甲基溴核心设施的目标是提供高质量的仪器， 广泛的基础和高级分子生物学领域的样品制备和培训 所有Cobre PJIS和其他由NIH资助的研究人员都可以使用这些技术来实现他们的研究目标。MB CORE确立了两个具体目标。目标1是为科布雷和其他由NIH资助的调查人员提供 分子和细胞生物学方面的资源和专业知识，包括仪器、技术支持、 咨询和培训。目标2是组织、协调和促进Cobre之间的互动 研究人员、导师和学生，促进新技术和化验的开发和使用，以及 对PJI教职员工和学生进行先进分子生物学技术培训。

PROJECT SUMMARY The Molecular Biology (MB) Core has been created to meet the need for advanced molecular biology support by PJIs. In particular, access to and experience in utilizing advanced molecular biology that are limited for most PJIs and established NIH-funded investigators. Specifically, MB Core will leverage and expand the resources and expertise of the Genelab Core facility which is comprised of three existing Core Facilities operated by the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM): Gene expression and sequencing Laboratory (GeneLab), Protein characterization and purification laboratory (PCPL), which is also responsible for generation of monospecific antibodies to proteins of interest, and the Molecular Immunopathology laboratory (MIPL) that provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These three laboratories are currently co-supported by the NIGMS-funded CEIDR-COBRE (Phase III) as well as the Louisiana Biomedical Research Network (LBRN/INBRE). The goal of the MB Core Facility of the Lung COBRE is to provide high quality instrumentation, sample preparation and training in a broad spectrum of fundamental and advanced molecular biology techniques to all COBRE PJIs and other NIH-funded investigators to achieve their research objectives. The MB Core has established 2 specific aims. Aim 1 is to provide the COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology, including instrumentation, technical support, consultation, and training. Aim 2 is to organize, coordinate, and facilitate interactions among COBRE investigators, mentors, and students, promote the development and use of new technologies and assays, and train PJI faculty, staff, and students in advanced molecular biology technologies.