Molecular Biology

项目摘要 分子生物学（MB）核心已经创建，以满足先进的分子生物学支持的需要 在PJI。特别是，获得和利用先进的分子生物学的经验是有限的， PJIs和NIH资助的研究人员。具体而言，MB Core将利用和扩展资源 Genelab核心设施由三个现有的核心设施组成， 生物技术和分子医学部（BioMMED），路易斯安那州立大学兽医学院 (SVM)：基因表达和测序实验室（GeneLab），蛋白质表征和纯化 实验室（PCPL），其也负责产生针对感兴趣蛋白的单特异性抗体， 和分子免疫病理学实验室（MIPL），提供专业知识和生物成像， 先进的免疫荧光和电子显微镜。这三个实验室目前共同支持 由NIGMS资助的CEIDR-COBRE（第三阶段）以及路易斯安那州生物医学研究网络 (LBRN/INBRE）。肺COBRE MB核心设施的目标是提供高质量的仪器， 在基础和先进的分子生物学的广泛的样品制备和培训 所有COBRE PJIs和其他NIH资助的研究人员都可以使用这些技术来实现他们的研究目标。的MB 核心设定了两个具体目标。目标1是为COBRE和其他NIH资助的研究人员提供 分子和细胞生物学方面的资源和专业知识，包括仪器，技术支持， 咨询和培训。目标2是组织、协调和促进COBRE之间的互动 研究人员、导师和学生，促进新技术和分析的开发和使用， 培训PJI的教职员工和学生先进的分子生物学技术。

PROJECT SUMMARY The Molecular Biology (MB) Core has been created to meet the need for advanced molecular biology support by PJIs. In particular, access to and experience in utilizing advanced molecular biology that are limited for most PJIs and established NIH-funded investigators. Specifically, MB Core will leverage and expand the resources and expertise of the Genelab Core facility which is comprised of three existing Core Facilities operated by the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM): Gene expression and sequencing Laboratory (GeneLab), Protein characterization and purification laboratory (PCPL), which is also responsible for generation of monospecific antibodies to proteins of interest, and the Molecular Immunopathology laboratory (MIPL) that provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These three laboratories are currently co-supported by the NIGMS-funded CEIDR-COBRE (Phase III) as well as the Louisiana Biomedical Research Network (LBRN/INBRE). The goal of the MB Core Facility of the Lung COBRE is to provide high quality instrumentation, sample preparation and training in a broad spectrum of fundamental and advanced molecular biology techniques to all COBRE PJIs and other NIH-funded investigators to achieve their research objectives. The MB Core has established 2 specific aims. Aim 1 is to provide the COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology, including instrumentation, technical support, consultation, and training. Aim 2 is to organize, coordinate, and facilitate interactions among COBRE investigators, mentors, and students, promote the development and use of new technologies and assays, and train PJI faculty, staff, and students in advanced molecular biology technologies.