Molecular Biology and Immunopathology Core

分子生物学和免疫病理学核心

• 批准号: 10360594
• 负责人: Konstantin G Kousoulas
• 金额: $ 30.67万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10360594
• 关键词: Adopted; Bioinformatics; Biological; Biological Assay; Biomedical Research; Biotechnology; CRISPR/Cas technology; Cell Culture Techniques; Cells; Cellular biology; Centers of Research Excellence; Collaborations; Computer software; Computers and Advanced Instrumentation; Consult; Consultations; Contract Services; Core Facility; Data; Disease; Disease model; Electron Microscopy; Ensure; Equipment; Experimental Designs; Faculty; Faculty Recruitment; Funding; Gene Expression; Genomics; Genus Hippocampus; Goals; Human; Human Resources; Immunofluorescence Microscopy; Immunologics; Immunology; Investigation; Laboratories; Leadership; Life; Louisiana; Maintenance; Malignant Neoplasms; Measurement; Measures; Methodology; Methods; Molecular; Molecular Biology; Molecular Medicine; Monoclonal Antibodies; Pathology; Patients; Productivity; Proteins; Proteomics; Quantitative Reverse Transcriptase PCR; Reagent; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Science; Services; Students; Technical Expertise; Technology; Time; Tissue Sample; Training; Training Activity; Training and Education; Treatment outcome; United States National Institutes of Health; Universities; Update; Veterinary Medicine; Veterinary Schools; Viral Vector; Work; animal imaging; animal tissue; anticancer research; base; bioimaging; cost; course module; experimental study; immunopathology; in vivo; instrumentation; interest; nano-string; new technology; novel therapeutic intervention; pre-clinical; preclinical evaluation; predictive modeling; repaired; single cell sequencing; transcriptome sequencing; transcriptomics

Summary Molecular Biology Immunopathology Core. The proposed Center for Pre-Clinical Cancer Research (Cancer COBRE) at Louisiana State University (LSU) focuses on elucidating molecular and cellular mechanisms of human cancer using specialized disease models that are predictive of disease in humans. The Cancer COBRE will focus on devastating human cancers that have poor treatment outcome in patients in need of novel therapeutic strategies. The proposed projects led by highly Promising Junior Investigators (PJIs) require the application of advanced molecular biological and immunopathological principles, methods and particularly advances in genomics, transcriptomics and proteomics, immunology and pathology to delineate key cellular mechanisms for each specific cancer necessitating access to state-of-the-art equipment and technical expertise. The Molecular Biology and Immunopathology Core (MBIPC) has been created to leverage and expand the resources and expertise of the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM), which administers six Core Facilities: Gene Expression and Sequencing Laboratory (GeneLab), Viral Vectors Laboratory, Protein Characterization and Purification laboratory (PCPL), which is also generates monospecific and monoclonal antibodies to proteins of interest, the Small Animal Imaging Laboratory (SAIL) and the Molecular Immunopathology laboratory (MIPL) that provides expertise in immunopathology and access to the SVM Centralized Facilities (FACS and Pathology Cost Centers), and also provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These laboratories are currently co-supported by the Louisiana Biomedical Research Network (LBRN/INBRE), which also operates the LBRN Bioinformatics Core in close collaboration with the LSU Center for Computation & Technology (CCT). The goal of MBIPC is to provide and coordinate access to state of the advanced instrumentation and services and provide training in a broad spectrum of fundamental and advanced molecular biology and immunopathology to all PJIs and other NIH- funded investigators to achieve their research objectives. MBIPC has identified two specific aims: Aim 1. To provide the Cancer COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology and immunopathology including instrumentation, technical support, consultation and training, and assistance in developing new methodologies. Aim 2: To organize, coordinate, and facilitate interactions among COBRE investigators and work collaboratively to develop and use new technologies and assays, and train PJIs, faculty, staff, and students in advanced molecular biology and immunopathology technologies.

分子生物学免疫病理学核心概要。 路易斯安那州立大学（LSU）的临床前癌症研究中心（Cancer COBRE） 重点是使用专门的疾病模型阐明人类癌症的分子和细胞机制 可以预测人类的疾病癌症COBRE将专注于破坏性的人类癌症， 需要新治疗策略的患者的治疗结果较差。由高度领导的项目 有前途的初级研究者（PJIs）需要应用先进的分子生物学和 免疫病理学原理、方法，特别是基因组学、转录组学和蛋白质组学的进展， 免疫学和病理学来描述每一种需要进入的特定癌症的关键细胞机制 最先进的设备和专业技术分子生物学和免疫病理学核心 （MBIPC）的建立是为了利用和扩大生物技术司的资源和专业知识， 和路易斯安那州立大学兽医学院（SVM）的分子医学（BioMMED），该学院管理六个 核心设施：基因表达和测序实验室（GeneLab），病毒载体实验室，蛋白质 表征和纯化实验室（PCPL），该实验室还生成单特异性和单克隆 小动物成像实验室（SAIL）和分子生物学实验室（Molecular Biological Laboratory） 免疫病理学实验室（MIPL），提供免疫病理学方面的专业知识和SVM访问 集中设施（流式细胞仪和病理成本中心），还提供专业知识和生物成像 先进的免疫荧光和电子显微镜。这些实验室目前得到以下机构的共同支持： 路易斯安那州生物医学研究网络（LBRN/INBRE），该网络还运营着LBRN生物信息学核心 与LSU计算与技术中心（CCT）密切合作。MBIPC的目标是提供 并协调先进仪器和服务的状态访问，并提供广泛的培训， 基础和先进的分子生物学和免疫病理学的频谱，所有PJIs和其他NIH- 资助研究人员实现他们的研究目标。MBIPC确定了两个具体目标： 目标1.为癌症COBRE和其他NIH资助的研究人员提供以下资源和专业知识： 分子和细胞生物学和免疫病理学，包括仪器，技术支持，咨询和 培训和协助制定新的方法。 目标2：组织、协调和促进COBRE调查人员之间的互动，并协同工作 开发和使用新技术和分析，并培训PJIs，教师，员工和学生在先进的 分子生物学和免疫病理学技术。